| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
N4-Acetylcytidine triphosphate sodium (0.1-2 mM; 24-48 h) suppresses BV2 microglia development [1]. N4-Acetylcytidine triphosphate sodium (0.3–1 mM; 3–6 h) increases the expression of NLRP3 and initiates NFκB signaling [1]. HMGB1 expression is elevated by N4-acetylcytidine triphosphate sodium (0.3–1 mM; 3 h) [1]. (br>
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|---|---|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: BV2 microglia Tested Concentrations: 0.1-2 mM Incubation Duration: 24-48 hrs (hours) Experimental Results: Dramatically inhibited BV2 microglia cell growth util cultured for 48 hrs (hours) with a concentration of 0.3 mM. Cell Viability Assay [1] Cell Types: BV2 microglia Tested Concentrations: 0.3 and 1 mM Incubation Duration: 3 and 6 hrs (hours) Experimental Results: Increased NLRP3 and NFκB expression at both mRNA and protein levels, activated the NFκB signaling. Western Blot Analysis[1] Cell Types: BV2 microglia Tested Concentrations: 0.3 and 1 mM Incubation Duration: 3 hrs (hours) Experimental Results: Induced HMGB1 expression and it is necessary for NFkB signaling, NLRP3 expression and persistent HMGB1 expression. |
| References |
| Molecular Formula |
C11H14N3NA4O15P3
|
|---|---|
| Molecular Weight |
613.120329380035
|
| Exact Mass |
612.922
|
| CAS # |
2803886-33-9
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| Related CAS # |
N4-Acetylcytidine triphosphate;1428903-57-4
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| PubChem CID |
146673014
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| Appearance |
White to yellow solid powder
|
| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
9
|
| Heavy Atom Count |
36
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| Complexity |
980
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
P(=O)([O-])(OP(=O)([O-])OP(=O)([O-])[O-])OC[C@@H]1[C@H]([C@H]([C@H](N2C(N=C(C=C2)NC(C)=O)=O)O1)O)O.[Na+].[Na+].[Na+].[Na+]
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| InChi Key |
VSBLTNBKSQTQJC-XGZYCNACSA-J
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| InChi Code |
InChI=1S/C11H18N3O15P3.4Na/c1-5(15)12-7-2-3-14(11(18)13-7)10-9(17)8(16)6(27-10)4-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,6,8-10,16-17H,4H2,1H3,(H,22,23)(H,24,25)(H2,19,20,21)(H,12,13,15,18);;;;/q;4*+1/p-4/t6-,8-,9-,10-;;;;/m1..../s1
|
| Chemical Name |
tetrasodium;[[[(2R,3S,4R,5R)-5-(4-acetamido-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] phosphate
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. (3). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O :~100 mg/mL (~163.10 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (40.78 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with heating and sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6310 mL | 8.1550 mL | 16.3100 mL | |
| 5 mM | 0.3262 mL | 1.6310 mL | 3.2620 mL | |
| 10 mM | 0.1631 mL | 0.8155 mL | 1.6310 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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