| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
DNA/RNA polymerases and transcription factors. N4-Acetylcytidine triphosphate is a substrate for RNA polymerases and can be incorporated into RNA transcripts. Once incorporated, the N4-acetyl modification (ac4C) influences RNA structure, stability, and translation efficiency.
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| ln Vitro |
N4-Acetylcytidine triphosphate sodium (0.1-2 mM; 24-48 h) suppresses BV2 microglia development [1]. N4-Acetylcytidine triphosphate sodium (0.3–1 mM; 3–6 h) increases the expression of NLRP3 and initiates NFκB signaling [1]. HMGB1 expression is elevated by N4-acetylcytidine triphosphate sodium (0.3–1 mM; 3 h) [1]. (br>
The compound itself has no pharmacological activity; it is a biochemical reagent. In in vitro transcription reactions, it replaces CTP to produce ac4C-modified RNA. The modified RNA has been shown to enhance translation efficiency and RNA stability in cellular assays. |
| ln Vivo |
No in vivo activity is reported for the compound directly. However, ac4C-modified mRNA (produced using ac4CTP) transfected into cells or animals can lead to increased protein expression compared to unmodified mRNA, making it useful for mRNA-based therapies.
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| Enzyme Assay |
A non-cell transcription assay is performed using T7 RNA polymerase, a DNA template, and NTP mix where CTP is partially or fully replaced by ac4CTP (0.5-2 mM). The reaction is incubated at 37degC for 2-4 hours. RNA products are analyzed by denaturing PAGE or by LC-MS to confirm ac4C incorporation.
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: BV2 microglia Tested Concentrations: 0.1-2 mM Incubation Duration: 24-48 hrs (hours) Experimental Results: Dramatically inhibited BV2 microglia cell growth util cultured for 48 hrs (hours) with a concentration of 0.3 mM. Cell Viability Assay [1] Cell Types: BV2 microglia Tested Concentrations: 0.3 and 1 mM Incubation Duration: 3 and 6 hrs (hours) Experimental Results: Increased NLRP3 and NFκB expression at both mRNA and protein levels, activated the NFκB signaling. Western Blot Analysis[1] Cell Types: BV2 microglia Tested Concentrations: 0.3 and 1 mM Incubation Duration: 3 hrs (hours) Experimental Results: Induced HMGB1 expression and it is necessary for NFkB signaling, NLRP3 expression and persistent HMGB1 expression. For cell studies, RNA transcripts synthesized with ac4CTP are transfected into mammalian cells (e.g., HEK293) using lipofection. After 6-24 hours, protein expression from the modified mRNA is measured by Western blot or luciferase assay. ac4C-modified mRNA typically shows higher translation efficiency. |
| Animal Protocol |
No animal studies are intended for the free nucleotide. However, ac4C-modified mRNA therapeutics (prepared using ac4CTP) have been tested in animal models. For example, modified mRNA encoding a therapeutic protein is injected intravenously or intramuscularly, and protein expression and efficacy are assessed.
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| ADME/Pharmacokinetics |
The compound is a highly polar nucleotide with three phosphate groups, stabilized as a sodium salt to improve water solubility. It is typically dissolved in nuclease-free water or TE buffer (pH 7.5) at 10-100 mM. It is stable for months at -20degC.
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| Toxicity/Toxicokinetics |
As a chemical reagent, ac4CTP sodium has low toxicity at the concentrations used for in vitro transcription (1-2 mM). It is not intended for direct administration to animals. Standard laboratory safety precautions for handling nucleotides should be followed.
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| References | |
| Additional Infomation |
N4-Acetylcytidine triphosphate sodium is used in research to synthesize RNA transcripts containing the epitranscriptomic mark N4-acetylcytidine (ac4C). It allows researchers to study how ac4C affects RNA stability, translation efficiency, and protein-protein interactions. It is a tool for mRNA therapeutics development. For research use only.
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| Molecular Formula |
C11H14N3NA4O15P3
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|---|---|
| Molecular Weight |
613.120329380035
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| Exact Mass |
612.922
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| CAS # |
2803886-33-9
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| Related CAS # |
N4-Acetylcytidine triphosphate;1428903-57-4
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| PubChem CID |
146673014
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| Appearance |
White to yellow solid powder
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
36
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| Complexity |
980
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| Defined Atom Stereocenter Count |
4
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| SMILES |
P(=O)([O-])(OP(=O)([O-])OP(=O)([O-])[O-])OC[C@@H]1[C@H]([C@H]([C@H](N2C(N=C(C=C2)NC(C)=O)=O)O1)O)O.[Na+].[Na+].[Na+].[Na+]
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| InChi Key |
VSBLTNBKSQTQJC-XGZYCNACSA-J
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| InChi Code |
InChI=1S/C11H18N3O15P3.4Na/c1-5(15)12-7-2-3-14(11(18)13-7)10-9(17)8(16)6(27-10)4-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,6,8-10,16-17H,4H2,1H3,(H,22,23)(H,24,25)(H2,19,20,21)(H,12,13,15,18);;;;/q;4*+1/p-4/t6-,8-,9-,10-;;;;/m1..../s1
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| Chemical Name |
tetrasodium;[[[(2R,3S,4R,5R)-5-(4-acetamido-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. (3). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~100 mg/mL (~163.10 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (40.78 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with heating and sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6310 mL | 8.1550 mL | 16.3100 mL | |
| 5 mM | 0.3262 mL | 1.6310 mL | 3.2620 mL | |
| 10 mM | 0.1631 mL | 0.8155 mL | 1.6310 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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