| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
DNA-dependent RNA polymerases I and II. By binding to the active site of the RNA polymerase, 3'-dUTP acts as a competitive inhibitor against the natural substrate UTP. It inhibits the incorporation of UTP into the elongating RNA chain, effectively blocking transcription. This makes it a valuable tool for studying the mechanics of RNA synthesis.
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| ln Vitro |
3'-Deoxyuridine-5'-triphosphate(3'-dUTP) is effectively synthesized, beginning with cordycepin. UTP incorporation into RNA by RNA polymerases is significantly and competitively inhibited by 3'-deoxyuridine-5'-triphosphate. Because it has no influence on poly(rA) synthesis, 3'-Deoxyuridine-5'-triphosphate will be helpful in molecular investigations on the connection of template and substrate in RNA synthesis with chromatin, isolated nuclei, or permeable cells[1].
In vitro, 3'-Deoxyuridine-5'-triphosphate is a strong and competitive inhibitor of RNA polymerases I and II. It inhibits the incorporation of UTP into RNA with a Ki value of 2.0 microM. The analogue is synthesized from cordycepin and has been used to study transcription mechanisms in purified enzyme systems. |
| ln Vivo |
The compound is not used for therapeutic in vivo applications. As a nucleotide analogue, it is used to study transcription in cell-free systems or permeabilized cells. Its ability to inhibit transcription is strictly an experimental tool, and it is not intended for use in whole animals due to its broad mechanism of toxicity.
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| Enzyme Assay |
A non-cell enzyme inhibition assay is performed using purified RNA polymerase I or II from Dictyostelium discoideum cells. The enzyme is incubated with DNA template, NTPs (including [3H]-UTP), and varying concentrations of 3'-dUTP. After incubation, the incorporation of radioactivity into RNA is measured. The Ki (2.0 microM) is determined using Dixon plot analysis.
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| Cell Assay |
3'-Deoxyuridine-5'-triphosphate is not used in standard cell viability assays. Its mechanism is too broad for specific cellular targeting. It is used in lysed or permeabilized cell systems to study the transcription machinery. For example, it can be added to isolated nuclei to block active transcription, serving as a control for studying RNA processing.
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| Animal Protocol |
3'-dUTP is not typically used for in vivo animal experiments due to its high toxicity and lack of selectivity. It is a tool for in vitro biochemistry. The compound is used to characterize the activity of purified RNA polymerases and to validate that an observed RNA synthesis is mediated by polymerases I and II.
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| ADME/Pharmacokinetics |
The compound has a molecular weight of 492.10 (as free acid) and is supplied as a trisodium salt. It is highly soluble in water (PBS). For long-term storage, it should be stored at -80degC to prevent degradation. It is stable in aqueous solution at neutral pH for short-term use. Formulation is not required for in vitro use.
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| Toxicity/Toxicokinetics |
3'-dUTP is considered a hazardous chemical due to its ability to inhibit transcription. It is non-toxic at the low concentrations used in cell-free assays (uM range), but it is not intended for live animal use. It is for research use only and is classified as a controlled substance for research purposes. Comprehensive human toxicology data is not applicable.
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| References | |
| Additional Infomation |
3'-Deoxyuridine-5'-triphosphate trisodium is a research-grade biochemical tool for studying transcription. It is not a clinical drug, has no FDA approval, and is not intended for human therapeutic use. It is a valuable reagent for investigating the kinetics and mechanisms of RNA polymerases I and II. It is strictly for research use only (RUO).
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| Molecular Formula |
C9H12N2NA3O14P3
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|---|---|
| Molecular Weight |
534.09
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| Related CAS # |
3'-Deoxyuridine-5'-triphosphate;69199-40-2
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| Appearance |
Solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~20 mg/mL (~37.45 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (93.62 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8723 mL | 9.3617 mL | 18.7234 mL | |
| 5 mM | 0.3745 mL | 1.8723 mL | 3.7447 mL | |
| 10 mM | 0.1872 mL | 0.9362 mL | 1.8723 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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