| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 25mg |
|
||
| Other Sizes |
| Targets |
Antennapedia Peptide TFA targets cell membranes, facilitating the intracellular delivery of cargo molecules. As a cell-penetrating peptide, it does not have a specific pharmacological receptor but rather mediates the translocation of conjugated molecules across cellular membranes.
|
|---|---|
| ln Vitro |
RQIKIWFQNRRMKWKK is the 16 amino acid peptide that makes up Antennapedia Peptide TFA. A 60 amino acid protein called the DNA binding domain of the Drosophila transcription factor, or Antennapedia, is quickly absorbed by cells and has been fused to certain antigens to increase their immunogenicity. Antennapedia Peptide TFA, a 16-amino acid peptide, has the ability to promote the cytoplasmic absorption of cytotoxic T cell epitope 9-mer peptides[1].
In vitro, Antennapedia Peptide TFA promotes the cytoplasmic absorption of cytotoxic T cell epitope 9-mer peptides. It is used to deliver antigens and other cargo molecules into cells for immunological and therapeutic applications. |
| ln Vivo |
In vivo, Antennapedia Peptide TFA can be used to deliver conjugated cargo molecules to cells and tissues. Its cell-penetrating properties make it a valuable tool for studying intracellular delivery of therapeutics and antigens.
|
| Enzyme Assay |
Non-cellular assays for Antennapedia Peptide TFA are not typically performed as it is a cell-penetrating peptide that functions by interacting with cellular membranes. Its activity is assessed in cell-based systems by measuring the uptake of fluorescently labeled peptide or cargo.
|
| Cell Assay |
In vitro cellular assays for Antennapedia Peptide TFA involve treating cells with fluorescently labeled peptide or peptide-cargo conjugates. Cellular uptake is quantified by flow cytometry or fluorescence microscopy. The peptide's ability to deliver cargo to the cytoplasm is confirmed by assessing the biological activity of the delivered molecule.
|
| Animal Protocol |
In vivo animal experiments for Antennapedia Peptide TFA would involve administering the peptide, typically conjugated to a cargo, to animal models. Its ability to deliver the cargo to specific tissues and elicit a biological response would be evaluated.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties for Antennapedia Peptide TFA are not detailed in available sources. As a cell-penetrating peptide, its PK would be influenced by factors such as stability, proteolytic degradation, and clearance. Its ability to cross biological membranes is a key feature.
|
| Toxicity/Toxicokinetics |
Toxicological data for Antennapedia Peptide TFA are not provided in available sources. As a research peptide, its safety profile has not been extensively characterized. It is intended for research use only and not for human consumption.
|
| References |
[1]. Pietersz GA, et al. A 16-mer peptide (RQIKIWFQNRRMKWKK) from antennapedia preferentially targets the Class I pathway. Vaccine. 2001 Jan 8;19(11-12):1397-405.
|
| Additional Infomation |
Antennapedia Peptide TFA (Penetratin peptide TFA) is a 16-amino acid cell-penetrating peptide derived from the Drosophila Antennapedia homeodomain. It promotes the cytoplasmic absorption of cargo molecules. It is supplied for research purposes only.
|
| Molecular Formula |
C104H168N34O20S.C2HF3O2
|
|---|---|
| Molecular Weight |
2360.82
|
| Related CAS # |
Antennapedia Peptide;188842-14-0
|
| Appearance |
Typically exists as solid at room temperature
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O :~100 mg/mL (~42.36 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (21.18 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.4236 mL | 2.1179 mL | 4.2358 mL | |
| 5 mM | 0.0847 mL | 0.4236 mL | 0.8472 mL | |
| 10 mM | 0.0424 mL | 0.2118 mL | 0.4236 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
