Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
The 6-mer synthetic peptide AT-1002 is part of a newly discovered class of drugs that can reversibly enhance the paracellular transport of molecules over the epithelial barrier. Cys-Cys dimerization is possible for AT-1002[1]. Cell viability is assessed by measuring the cellular ATP concentration, and undifferentiated Caco-2 cells are assessed using AT-1002 (0 to 5 mg/mL, 3 or 24 hours). Cell viability at any concentration is unaffected by treatment as measured by AT-1002 for up to three hours. Specifically, 5 mg/mL of AT-1002 had no effect on the viability of Caco-2 cells. At doses of 2.5 mg/mL and above, AT-1002 decreases cell viability after 24 hours. However, washing the cells after they have been exposed to AT-1002 for three hours does not cause irreversible damage to the cells, as the cells still function after twenty-four hours[2].
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Cell Assay |
Cell Viability Assay[2]
Cell Types: Caco-2 cells Tested Concentrations: 0 to 5 mg/mL Incubation Duration: 3 or 24 hrs (hours) Experimental Results: Treatment for up to 3 h did not affect cell viability at any concentration. decreased cell viability after 24 h at concentrations of 2.5 mg/mL and higher. |
References |
[1]. Li M, et al. Structure-activity relationship studies of permeability modulating peptide AT-1002. Bioorg Med Chem Lett. 2008 Aug 15;18(16):4584-6.
[2]. Gopalakrishnan S, et al. Mechanism of action of ZOT-derived peptide AT-1002, a tight junction regulator and absorption enhancer. Int J Pharm. 2009 Jan 5;365(1-2):121-30. |
Molecular Formula |
C34H54F3N9O9S
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Molecular Weight |
821.91
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Related CAS # |
AT-1002;835872-35-0
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Solubility (In Vitro) |
DMSO :~33.33 mg/mL (~40.55 mM)
H2O :~1 mg/mL (~1.22 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.04 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.04 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (3.04 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 8.33 mg/mL (10.13 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.2167 mL | 6.0834 mL | 12.1668 mL | |
5 mM | 0.2433 mL | 1.2167 mL | 2.4334 mL | |
10 mM | 0.1217 mL | 0.6083 mL | 1.2167 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.