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| Targets |
BIOTIN-PEG11-SH does not target a specific biological receptor; rather, it provides two functional groups for conjugation. The biotin group targets streptavidin or avidin with high affinity and specificity, while the thiol group targets maleimide-activated molecules or gold surfaces for conjugation. The specific 11-unit PEG spacer provides a water-soluble, flexible, and non-immunogenic bridge, reducing steric hindrance between the labeled components.
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| ln Vitro |
In vitro, BIOTIN-PEG11-SH is used for the biotinylation and functionalization of proteins and nanoparticles. It is not typically used in cell viability or signaling assays, as it is not a modulator of biological activity. Instead, it serves as a tool for labeling. The PEG11 spacer ensures that the biotin tag is accessible for streptavidin binding even when the conjugated protein is folded or complexed.
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| ln Vivo |
In vivo activity data for BIOTIN-PEG11-SH have not been reported. As an activated linker for bioconjugation, the compound is designed for in vitro use in molecular biology and diagnostics. It is not intended for in vivo administration as a therapeutic agent, and no animal efficacy or pharmacokinetic studies are standard for this type of reagent.
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| Enzyme Assay |
Non-cellular (in vitro) binding assays using BIOTIN-PEG11-SH involve standard maleimide-thiol coupling. For protein labeling, the compound is dissolved in a degassed PBS buffer (pH 7.2-7.5). It is then incubated with a protein that has been modified to contain maleimide groups. The reaction proceeds for 2-4 hours at room temperature. The resulting biotinylated protein can be visualized via a streptavidin-HRP conjugate on a Western blot.
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| Cell Assay |
For cellular assays, BIOTIN-PEG11-SH is first conjugated to a cell-impermeable maleimide dye or a targeting ligand. The resulting biotin-labeled probe is incubated with live cells for 30-60 minutes. After washing, cells are incubated with a streptavidin-fluorophore conjugate. The surface labeling efficiency is then assessed using flow cytometry or fluorescence microscopy. Due to its membrane impermeability, it is primarily used for extracellular labeling.
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| Animal Protocol |
Detailed in vivo animal experimental protocols for BIOTIN-PEG11-SH are not described in the literature. The compound's application is strictly as a research tool for in vitro bioconjugation. In vivo administration studies are not standard for this type of biotinylation reagent, and no specific dosing or animal model data are available from standard product documentation.
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| ADME/Pharmacokinetics |
Dedicated pharmacokinetic (PK) studies for BIOTIN-PEG11-SH are not applicable. As a highly polar and reactive linker, it is not expected to cross cell membranes. It is primarily used for biotinylating biomolecules in vitro. The PEG11 spacer confers high water solubility, and the thiol group is reactive and not stable in circulation. It would likely be rapidly cleared if administered systemically.
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| Toxicity/Toxicokinetics |
Comprehensive toxicological data for BIOTIN-PEG11-SH are not reported in standard product literature. As a research-use linker, standard safety assessments for acute toxicity, genotoxicity, and organ-specific toxicity are not typically described. For laboratory use, standard chemical safety precautions for handling PEG linkers should be followed. It should be stored protected from moisture and under an inert atmosphere.
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| Additional Infomation |
BIOTIN-PEG11-SH (CAS: 1650579-23-9) has a molecular formula of C34H65N3O13S2 and a molecular weight of 788.02 g/mol. The IUPAC name is N-(35-mercapto-3,6,9,12,15,18,21,24,27,30,33-undecaoxapentatriacontyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide. It appears as an off-white to light yellow solid powder with a purity of ≥98%. The compound is typically stored at -20degC, sealed, and protected from moisture.
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| Molecular Formula |
C34H65N3O13S2
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| Molecular Weight |
788.02
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| CAS # |
1650579-23-9
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.151±0.06 g/cm3(Predicted)
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| Boiling Point |
879.8±65.0 °C(Predicted)
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| LogP |
0
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :≥ 100 mg/mL (~126.90 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.17 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.17 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (3.17 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2690 mL | 6.3450 mL | 12.6900 mL | |
| 5 mM | 0.2538 mL | 1.2690 mL | 2.5380 mL | |
| 10 mM | 0.1269 mL | 0.6345 mL | 1.2690 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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