| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
CY7-N3 does not bind to a biological target; it is a chemical labeling reagent. The azide group (-N3) enables bioorthogonal conjugation via click chemistry reactions with alkyne, DBCO, or BCN functional groups. The CY7 fluorophore provides NIR fluorescence for detection and imaging. This click chemistry approach allows labeling of biomolecules without interfering with biological functions.
|
|---|---|
| ln Vitro |
In vitro, CY7-N3 is used as a fluorescent labeling agent via click chemistry. For CuAAc, the alkyne-bearing molecule is incubated with CY7-N3 (1-5 equivalents) in the presence of CuSO4 (1 mM) and sodium ascorbate (2-5 mM) in PBS buffer (pH 7.4) containing 10-20% DMSO for 1-12 hours at 37degC. For SPAAC, CY7-N3 is mixed with DBCO- or BCN-conjugated molecules (1:1 ratio) in PBS (pH 7.4) for 2-4 hours at 37degC or overnight at 4degC. The labeled products are purified by dialysis or size-exclusion chromatography and used for downstream applications such as fluorescence microscopy or flow cytometry.
|
| ln Vivo |
CY7-N3 is not administered directly for in vivo activity. It is used to label targeting ligands (e.g., antibodies, peptides, nanoparticles) through click chemistry. The CY7-labeled conjugates are then administered intravenously to animal models for NIR fluorescence imaging, allowing non-invasive tracking of biodistribution, tumor targeting, and cellular processes. The water solubility of CY7-N3 facilitates formulation for conjugation reactions and subsequent in vivo administration of labeled conjugates.
|
| Enzyme Assay |
For in vitro click chemistry conjugation, CY7-N3 is not used in enzyme-binding assays. Standard conjugation protocol: For CuAAc, dissolve 1 micromol of alkyne-functionalized biomolecule in 100 microL of degassed PBS (pH 7.4). Add 1-1.5 micromol CY7-N3 (1-1.5 equivalents), 100 microL of 2 mM CuSO4 (final 1 mM), and 100 microL of 10 mM sodium ascorbate (final 5 mM). Incubate at 37degC for 4-12 hours with gentle shaking. For SPAAC, add 1 micromol DBCO-conjugated molecule to 100 microL PBS (pH 7.4), then add 1-1.2 micromol CY7-N3 in 100 microL PBS (10-20% DMSO). Incubate at 37degC for 2-4 hours. Monitor conjugation by TLC (silica gel, 10% methanol in dichloromethane) or by LC-MS. Purify by size-exclusion chromatography (e.g., Sephadex G-25 column) or dialysis (3.5-10 kDa cutoff). Determine labeling efficiency by measuring absorbance at 750 nm (CY7) and 280 nm (protein).
|
| Cell Assay |
For cell-based assays, CY7-labeled biomolecules are used in cellular uptake studies. Cells (e.g., 1-2×10⁵ cells/well) are seeded in 8-well chamber slides and cultured overnight. CY7-labeled probe is added to culture medium at 1-10 microg/mL (based on protein concentration) and incubated for 1-4 hours at 37degC. Cells are washed with PBS (3×), fixed with 4% paraformaldehyde (15 min, room temperature), counterstained with Hoechst 33342 for nuclei, and mounted. Fluorescence imaging is performed with excitation/emission at 750/775 nm. For viability assessment, cells treated with the labeled probe are analyzed using standard MTT or CellTiter-Glo assays to ensure no non-specific cytotoxicity.
|
| Animal Protocol |
For in vivo animal studies, CY7-labeled conjugates are typically administered via intravenous injection (tail vein) to mice bearing subcutaneous tumor xenografts. Dose: 50-100 microg of labeled protein (e.g., antibody) per mouse in 100-200 microL PBS (pH 7.4). In vivo NIR fluorescence imaging is performed at 1, 6, 12, 24, 48, and 72 hours post-injection using an imaging system with excitation at 745-755 nm and emission at 800 nm bandpass filter. At the terminal time point (e.g., 72 hours), mice are euthanized, and organs (tumor, liver, spleen, kidneys, lungs, heart) are collected for ex vivo imaging to quantify biodistribution. Tumor-to-background ratio is calculated by dividing fluorescence signal in the tumor by signal in adjacent normal tissue (e.g., muscle). Pharmacokinetic parameters (AUC, half-life) can be determined by blood sampling at multiple time points (e.g., 5 min, 1, 4, 8, 24, 48 h) and ex vivo fluorescence measurement in plasma.
|
| ADME/Pharmacokinetics |
CY7-N3 has a molecular weight of 763.95 Da. The compound is supplied as a solid (typically yellow to dark blue powder) and should be stored at -20degC, protected from light. Solubility: DMSO (80 mg/mL, 104.72 mM); water (soluble due to sulfonate groups). For in vivo formulations, a common vehicle is 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline, which yields a clear solution at 3.3 mg/mL (4.32 mM). Working solutions should be prepared fresh and used immediately. Store stock solutions at -80degC for up to 6 months and avoid repeated freeze-thaw cycles.
|
| Toxicity/Toxicokinetics |
CY7-N3 is a water-soluble NIR dye azide for click chemistry. It is considered non-toxic at typical research concentrations (microM to low mM range for conjugation reactions). However, the compound should be handled with care (use of gloves, lab coat, and eye protection). This product is for research use only and not for human diagnostic or therapeutic applications. No significant acute toxicity has been reported. For in vivo studies, standard safety monitoring (body weight, behavior) should be performed.
|
| Additional Infomation |
CY7-N3 (Sulfo-Cyanine7-N3) is classified as a NIR fluorescent dye and click chemistry reagent. Key features: (1) CY7 fluorophore with excitation/emission maximum at approximately 750/775 nm, enabling deep-tissue imaging; (2) Sulfonate groups provide excellent water solubility, eliminating the need for organic solvents in conjugation reactions; (3) Azide group enables bioorthogonal conjugation via CuAAc or SPAAC click reactions; (4) Water-soluble NIR dye azide is suitable for cell imaging through click reactions. Purity is typically ≥97% by HPLC. Common applications: fluorescent labeling of proteins, antibodies, nucleic acids, and nanoparticles for in vitro and in vivo imaging. This product is not approved for clinical use.
|
| Molecular Formula |
C38H47N6O7S2
|
|---|---|
| Molecular Weight |
763.95
|
| Appearance |
Purple to purplish red solid powder
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO :~100 mg/mL (~130.90 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.27 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.27 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3090 mL | 6.5449 mL | 13.0899 mL | |
| 5 mM | 0.2618 mL | 1.3090 mL | 2.6180 mL | |
| 10 mM | 0.1309 mL | 0.6545 mL | 1.3090 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
