| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg | |||
| Other Sizes |
| Targets |
Reacts with cysteine thiols (via iodoacetamide group) and binds to streptavidin/avidin (via desthiobiotin).
|
|---|---|
| ln Vitro |
Used as a probe to investigate cysteine reactivity; labels reduced cysteine residues in proteins via a stable thioether linkage; desthiobiotin tag enables affinity capture with mild elution using free biotin.
|
| ln Vivo |
No in vivo activity data are available; the compound is a chemical probe for in vitro cysteine modification and proteomics applications.
|
| Enzyme Assay |
Labeling efficiency is assessed by incubating reduced protein samples with desthiobiotin-iodoacetamide; desthiobiotinylated proteins are captured on streptavidin beads, eluted with excess free biotin, and analyzed by SDS-PAGE or mass spectrometry.
|
| Cell Assay |
Cell lysates are treated with desthiobiotin-iodoacetamide to label reactive cysteine residues; labeled peptides are enriched by streptavidin beads and analyzed by LC-MS/MS for cysteine reactivity mapping in complex proteomes.
|
| Animal Protocol |
In vivo protocols are not applicable; for ex vivo studies, tissue lysates from treated animals can be labeled and analyzed to assess cysteine oxidation or drug-adduct formation.
|
| ADME/Pharmacokinetics |
PK studies are not performed for this reagent; the iodoacetamide group is reactive and unstable in solution, requiring freshly prepared formulations for labeling experiments.
|
| Toxicity/Toxicokinetics |
The iodoacetamide group is an alkylating agent and should be handled with care; the compound is for research use only and is not intended for in vivo administration.
|
| References | |
| Additional Infomation |
Desthiobiotin-Iodoacetamide is a research tool for chemoproteomics, cysteine profiling, and ADC linker development; it has no clinical trial or regulatory approval.
|
| Molecular Formula |
C14H25IN4O3
|
|---|---|
| Molecular Weight |
424.28
|
| Exact Mass |
424.097
|
| CAS # |
2924824-04-2
|
| PubChem CID |
166642567
|
| Appearance |
White to off-white solid powder
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
10
|
| Heavy Atom Count |
22
|
| Complexity |
392
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
C[C@H]1[C@H](NC(=O)N1)CCCCCC(=O)NCCNC(=O)CI
|
| InChi Key |
ASKKBTGJVCEXOA-WDEREUQCSA-N
|
| InChi Code |
InChI=1S/C14H25IN4O3/c1-10-11(19-14(22)18-10)5-3-2-4-6-12(20)16-7-8-17-13(21)9-15/h10-11H,2-9H2,1H3,(H,16,20)(H,17,21)(H2,18,19,22)/t10-,11+/m0/s1
|
| Chemical Name |
N-[2-[(2-iodoacetyl)amino]ethyl]-6-[(4R,5S)-5-methyl-2-oxoimidazolidin-4-yl]hexanamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO :~50 mg/mL (~117.85 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3569 mL | 11.7847 mL | 23.5693 mL | |
| 5 mM | 0.4714 mL | 2.3569 mL | 4.7139 mL | |
| 10 mM | 0.2357 mL | 1.1785 mL | 2.3569 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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