Size | Price | Stock | Qty |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
In neutral pH, tagged proteins are frequently isolated from cell culture supernatants and cell lysates using HA Peptide, a highly immunoreactive label. As such, Western blot provides a straightforward method of detection, making them useful instruments for coimmunoprecipitation. The fusion partner protein's biological activity and function are unlikely to be affected by the tiny size of HA Peptide. The HA Peptide is a useful tool for isolating, purifying, detecting, and tracking proteins of interest. It is derived from human influenza hemagglutinin (HA), which corresponds to amino acids 98–106. It is a powerfully immunoreactive epitope. Anti-HA monoclonal antibodies that are highly selective and covalently bound on resin can be used to isolate recombinant HA-tagged proteins. Using TBS containing 1 mg/mL of the HA epitope, HA-tagged proteins can be gently eluted. Three alternatives exist for chemical elution as well: 50 mM NaOH, 3 M NaSCN, and 0.1 M glycine (pH 2-2.8) [1]. For T7 promoter-driven expression in E. coli, even in the absence of transcriptionally active T7 RNAP, the nucleotide sequence encoding the N-terminal HA Peptide in mammalian expression vectors is crucial [2]. According to research findings, caspase 3/7 cleaves the HA peptide, which leaves the immune response completely destroyed. Studies on apoptotic pathways linked to cell death have shown that using HA-tagged proteins and constructs may result in significant artifacts [3].
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References |
[1]. Zhao X, et, al. Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem. 2013;2013:581093.
[2]. Moon JM, et, al. A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA tag in the T7 promoter-driven expression in E. coli. Biotechnol Lett. 2012 Oct;34(10):1841-6. [3]. Schembri L, et, al. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods. 2007 Feb;4(2):107-8. |
Molecular Formula |
C55H68F3N9O19
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Molecular Weight |
1216.17
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Related CAS # |
HA Peptide;92000-76-5
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO :≥ 100 mg/mL (~82.23 mM)
H2O :~50 mg/mL (~41.11 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: 1.25 mg/mL (1.03 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (1.03 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (1.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 0.8223 mL | 4.1113 mL | 8.2225 mL | |
5 mM | 0.1645 mL | 0.8223 mL | 1.6445 mL | |
10 mM | 0.0822 mL | 0.4111 mL | 0.8223 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.