| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg | |||
| Other Sizes |
| Targets |
Histone H2A (substrate). Histone H2A (1-20) is a synthetic peptide that models the N-terminal tail of histone H2A. It serves as a substrate for enzymes that modify lysine residues (acetylation, methylation, ubiquitination? Ubiquitination typically occurs on Lys119, which is not within 1-20). It is used to study acetylation by HATs (e.g., p300, CBP) and deacetylation by HDACs. It can also bind to bromodomains (acetylated forms). It is not a drug; it is a biochemical reagent.
|
|---|---|
| ln Vitro |
In vitro, the peptide (1-20) can be acetylated by p300 in the presence of acetyl-CoA. Acetylation can be detected by mass spectrometry or by anti-acetyl-lysine antibody. It can also be used in binding assays with bromodomain-containing proteins. The peptide is soluble. No specific activity data; it is a generic substrate.
|
| Enzyme Assay |
For acetylation assay, incubate Histone H2A (1-20) (10 uM) with p300 (0.1 uM) and acetyl-CoA (10 uM) in HAT buffer (50 mM Tris-HCl pH 8.0, 0.1 mM EDTA, 1 mM DTT) for 30-60 min at 30degC. Stop by adding 0.5% TFA. Analyze by LC-MS. For binding, immobilize acetylated peptide on plate, add bromodomain protein, detect with specific antibody.
|
| References |
[1]. Burgos ES, et, al. Histone H2A and H4 N-terminal tails are positioned by the MEP50 WD repeat protein for efficient methylation by the PRMT5 arginine methyltransferase. J Biol Chem. 2015 Apr 10; 290(15): 9674-89.
|
| Additional Infomation |
Histone H2A (1-20) is used for studies of the N-terminal tail of H2A, which is involved in transcriptional regulation and chromatin dynamics. For research only.
|
| Molecular Formula |
C83H155N37O26
|
|---|---|
| Molecular Weight |
2087.35
|
| Appearance |
Typically exists as solid at room temperature
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O :~100 mg/mL (~47.91 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (47.91 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.4791 mL | 2.3954 mL | 4.7908 mL | |
| 5 mM | 0.0958 mL | 0.4791 mL | 0.9582 mL | |
| 10 mM | 0.0479 mL | 0.2395 mL | 0.4791 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
