| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Histone H3 (substrate). Histone H3 (1-35) TFA is a synthetic peptide that serves as a model substrate for enzymes that modify histone tails, such as methyltransferases (e.g., SET1/MLL, EZH2, G9a), demethylases (e.g., KDM1A, KDM4, KDM5), acetyltransferases (HATs), deacetylases (HDACs), and kinases. It also binds to reader proteins that recognize specific modifications (e.g., methylated lysine). The peptide includes the N-terminal tail up to residue 35, which contains all the important modification sites. It does not have a specific pharmacological target; it is a biochemical tool.
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| ln Vitro |
Histone H3 is a crucial protein in the developing science of epigenetics, where it is believed that the dynamic and long-term regulation of genes is influenced by its sequence variants and variable modification states[1].
In vitro, Histone H3 (1-35) TFA is used as a substrate in enzyme assays. For example, it can be methylated by recombinant PRC2 (EZH2) at Lys27 in the presence of SAM, and the methylation can be detected by mass spectrometry or by using modification-specific antibodies. It can also be demethylated by KDM5A at Lys4. The peptide is soluble in water or buffer. It is not used for cell-based assays because it is not cell-permeable; it is used in cell-free systems. The TFA salt does not affect enzyme activity. The peptide can be biotinylated for pull-down assays. It is a standard reagent in epigenetics. |
| ln Vivo |
Not applicable. The peptide is not administered in vivo; it is a biochemical tool. It can be used to generate antibodies against histone H3 modifications, but the peptide itself is not a drug.
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| Enzyme Assay |
For a methyltransferase assay, incubate Histone H3 (1-35) TFA (1-10 uM) with purified EZH2 (PRC2) complex (0.1-1 uM) and S-adenosylmethionine (SAM, 1-10 uM) in reaction buffer (50 mM Tris-HCl pH 8.0, 10 mM MgCl2, 2 mM DTT) for 1-2 h at 37degC. Terminate by adding 0.5% TFA. Analyze by MALDI-TOF or LC-MS to detect mass increase due to methylation. For ELISA-based detection, coat the peptide on a plate and use anti-H3K27me3 antibody. For a binding assay, immobilize the peptide on a streptavidin plate (if biotinylated) and add a reader protein (e.g., CBX7), detect with specific antibody. The TFA salt is soluble; dissolve in water.
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| Cell Assay |
Not applicable (cell-free). However, the peptide can be electroporated into cells or used in nuclear extracts. For a cellular assay, prepare cell nuclear extracts, incubate with the peptide (10 uM) and SAM, then analyze methylation by Western blot with modification-specific antibodies. This is not standard. Typically, the peptide is used in vitro only.
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| Toxicity/Toxicokinetics |
No toxicity. The peptide is safe to handle with standard precautions.
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| References | |
| Additional Infomation |
Histone H3 (1-35) is a widely used substrate for studying histone modifications. It includes residues 1-35, covering the N-terminal tail that contains most of the regulatory sites (K4, K9, K14, K17, K18, K23, K27, K36). The TFA salt improves solubility. It is for research use only.
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| Molecular Formula |
C151H270F3N55O47
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|---|---|
| Molecular Weight |
3665.15
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| Related CAS # |
Histone H3 (1-35);135990-31-7
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| Appearance |
Solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~50 mg/mL (~13.64 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (27.28 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.2728 mL | 1.3642 mL | 2.7284 mL | |
| 5 mM | 0.0546 mL | 0.2728 mL | 0.5457 mL | |
| 10 mM | 0.0273 mL | 0.1364 mL | 0.2728 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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