| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
|
| ln Vivo |
Human TFA produces EAU in IRBP (1–20)[2]. 21 days following vaccination, lymph node and spleen cells are harvested, and the appropriate antigen is used to activate them in culture in order to measure proliferative and cytokine responses. The peptide caused human TFA and cells from C57BL/6 and 129/J animals vaccinated with IRBP (1–20) to proliferate[2].
|
|---|---|
| Animal Protocol |
Animal/Disease Models: C57BL/6 (H-2b) mice, H-2b (C57BL/6, 129/J ) mice[2]
Doses: 200, 300 μg Route of Administration: Ih; After 21 days Experimental Results: C57BL/6 (H-2b) mice immunized with the human peptide (200-300 μg= 80-120 nmoles) developed EAU. 129/ J mice (H-2b) also developed disease, albeit with a lower average score than C57BL/6. |
| References |
|
| Molecular Formula |
C103H165F3N24O30S
|
|---|---|
| Molecular Weight |
2308.62
|
| Related CAS # |
IRBP (1-20), human;298202-25-2
|
| Appearance |
Solid powder
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO :~100 mg/mL (~43.32 mM)
H2O :~8.33 mg/mL (~3.61 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (1.08 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.4332 mL | 2.1658 mL | 4.3316 mL | |
| 5 mM | 0.0866 mL | 0.4332 mL | 0.8663 mL | |
| 10 mM | 0.0433 mL | 0.2166 mL | 0.4332 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
