Size | Price | Stock | Qty |
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1mg |
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5mg |
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Other Sizes |
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ln Vitro |
Compound 11c, PD-L1-IN-2, inhibits RKO cells with an IC50 value of 31.7μM[1]. In RKO cells, PD-L1 expression is inhibited by PD-L1-IN-2 (0-10 μM; 0-24 hours) in a way that is dependent on both dose and time [1]. PD-L1-IN-2 (0–10 μM; 0–24 hours) increases the turnover of the PD-L1 protein. The PD-L1 turnover rate in PD-L1-IN-2-treated cells was higher in CHX pulse chase compared to untreated cells[1].
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ln Vivo |
GPR84 antagonist 3 (compound 42) (ip; 25/50 mg/kg; once daily; 16 days) had a 45% inhibition rate in the 50 mg/kg group, suggesting that it can diminish tumor size. Compared to the PBS group, the group's average tumor weight was noticeably reduced[1].
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Cell Assay |
Western Blot Analysis[1]
Cell Types: RKO cells Tested Concentrations: 5 μM Incubation Duration: 0h, 1h, 3h, 6h, 9h, 12h Experimental Results: Promoted the turnover of PD-L1 protein. |
Animal Protocol |
Animal/Disease Models: C57BL/6 mice with subcutaneous (sc) MC38 tumors[1]
Doses: 25/50 mg/kg Route of Administration: ip; 25/50 mg/kg; one time/day; 16 days Experimental Results: Suppressed MC38 tumor growth in vivo. |
References |
[1]. Pan-Pan Fu, et al. Bioactivity-Driven Synthesis of the Marine Natural Product Naamidine J and Its Derivatives as Potential Tumor Immunological Agents by Inhibiting Programmed Death-Ligand 1. J Med Chem. 2023 Apr 27;66(8):5427-5438.
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Molecular Formula |
C33H38N4O6
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Molecular Weight |
586.68
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CAS # |
2894733-91-4
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 50 mg/mL (85.23 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.26 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.7045 mL | 8.5225 mL | 17.0451 mL | |
5 mM | 0.3409 mL | 1.7045 mL | 3.4090 mL | |
10 mM | 0.1705 mL | 0.8523 mL | 1.7045 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.