| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
|
| Targets |
15-LOX-1 0.19 μM (IC50)
|
|---|---|
| ln Vitro |
Compound 9c, also known as 15-LOX-1 inhibitor 1 (0-5 μM; 24 hours) has dose-dependent effects that are more pronounced and result in a 20% increase in survivability for RAW 264.7 macrophages when exposed to LPS[1].
|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: RAW 264.7 macrophages Tested Concentrations: 0, 0.2, 1, 5 μM 15-LOX-1 inhibitor 1 and lipopolysaccharides (LPS) (100 μg/mL) Incubation Duration: 24 hrs (hours) Experimental Results: demonstrated stronger , dose-dependent effects with a 20% viability increase at 5 μM. |
| References |
[1]. Guo H, et al. Novel 15-Lipoxygenase-1 Inhibitor Protects Macrophages from Lipopolysaccharide-Induced Cytotoxicity. J Med Chem. 2019;62(9):4624-4637.
|
| Molecular Formula |
C22H21CLN2O4
|
|---|---|
| Molecular Weight |
412.87
|
| CAS # |
2349374-37-2
|
| SMILES |
ClC1C=CC2=C(C=1)NC(C(=O)OCC)=C2/C=C/C(NCC1C=CC=CC=1OC)=O
|
| Solubility (In Vitro) |
DMSO: 10 mg/mL (24.22 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1 mg/mL (2.42 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4221 mL | 12.1103 mL | 24.2207 mL | |
| 5 mM | 0.4844 mL | 2.4221 mL | 4.8441 mL | |
| 10 mM | 0.2422 mL | 1.2110 mL | 2.4221 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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