| Size | Price | Stock | Qty |
|---|---|---|---|
| 50g |
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| Other Sizes |
| Targets |
HD-cis-Hyp-OH does not have a specific pharmacological target as it is a research chemical rather than a therapeutic drug. Hydroxyproline itself is a component of collagen and is involved in collagen stability and structure. The compound may interact with enzymes involved in collagen metabolism, such as prolyl hydroxylase, but specific targets are not well defined.
|
|---|---|
| ln Vitro |
In vitro activity of HD-cis-Hyp-OH is not extensively characterized. As a hydroxyproline stereoisomer, it may be used to study collagen structure, protein folding, and the specificity of enzymes involved in proline hydroxylation. Its "activity" is reflected in its role as a research tool for studying protein structure and collagen metabolism.
|
| ln Vivo |
In vivo, HD-cis-Hyp-OH is not administered as a therapeutic agent. Hydroxyproline is a component of collagen and is found in connective tissues throughout the body. The compound may be used in research to study collagen metabolism, bone turnover, and connective tissue disorders. Its levels in urine and blood are used as markers of collagen turnover.
|
| Enzyme Assay |
In vitro enzyme assays with HD-cis-Hyp-OH typically involve studying enzymes involved in proline and hydroxyproline metabolism, such as prolyl hydroxylase or hydroxyproline dehydrogenase. The compound can be used as a substrate or inhibitor in assays to characterize the activity of these enzymes. Standard assays involve incubating with enzyme preparations and analyzing products by chromatographic methods.
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| Cell Assay |
HD-cis-Hyp-OH is not typically used in cell culture experiments as a bioactive compound. However, it may be employed in studies investigating collagen synthesis, protein folding, or amino acid metabolism. Cells such as fibroblasts or osteoblasts could be treated with the compound to study its effects on collagen production or cell function. Its primary use is as a research tool.
|
| Animal Protocol |
In vivo animal experiments with HD-cis-Hyp-OH are not commonly performed as the compound is a research chemical rather than a therapeutic agent. If used, it would be to study collagen metabolism or to validate analytical methods for measuring hydroxyproline isomers in biological samples. Its primary significance is in research as a reference standard.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of HD-cis-Hyp-OH are not characterized as the compound is a research chemical. As a small, polar amino acid derivative, it is expected to be absorbed and distributed throughout the body. It is likely metabolized through amino acid metabolic pathways and excreted in urine. Specific PK data are not available.
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| Toxicity/Toxicokinetics |
HD-cis-Hyp-OH has a low toxicity profile as it is an amino acid derivative. It is not considered a hazardous substance. For research use, standard laboratory safety practices are sufficient. Comprehensive toxicology studies have not been published for this compound. It is handled as a standard laboratory chemical.
|
| References | |
| Additional Infomation |
cis-4-hydroxy-D-proline is a 4-hydroxy-D-proline with an R configuration at the 4-position. It is a metabolite, specifically a mouse metabolite. It is a zwitterionic tautomer of cis-4-hydroxy-D-proline. Cis-4-hydroxy-D-proline has been reported in Daphnia pulex and Aeromonas veronii, with supporting data.
HD-cis-Hyp-OH (cis-4-hydroxy-D-proline) is a stereoisomer of hydroxyproline used as a research tool for studying collagen structure, protein folding, and amino acid metabolism. Hydroxyproline is a major component of collagen and is involved in collagen stability. The compound is not a drug and has no therapeutic indications. It is available for laboratory research use only. |
| Molecular Formula |
C5H9NO3
|
|---|---|
| Molecular Weight |
131.13
|
| Exact Mass |
131.058
|
| CAS # |
2584-71-6
|
| PubChem CID |
440014
|
| Appearance |
White to gray solid powder
|
| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
355.2±42.0 °C at 760 mmHg
|
| Melting Point |
243 °C (dec.)(lit.)
|
| Flash Point |
168.6±27.9 °C
|
| Vapour Pressure |
0.0±1.8 mmHg at 25°C
|
| Index of Refraction |
1.540
|
| LogP |
-1.84
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
9
|
| Complexity |
125
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
C1[C@H](CN[C@H]1C(=O)O)O
|
| InChi Key |
PMMYEEVYMWASQN-QWWZWVQMSA-N
|
| InChi Code |
InChI=1S/C5H9NO3/c7-3-1-4(5(8)9)6-2-3/h3-4,6-7H,1-2H2,(H,8,9)/t3-,4-/m1/s1
|
| Chemical Name |
(2R,4R)-4-hydroxypyrrolidine-2-carboxylic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 100 mg/mL (762.60 mM)
DMSO: 1 mg/mL (7.63 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (762.60 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.6260 mL | 38.1301 mL | 76.2602 mL | |
| 5 mM | 1.5252 mL | 7.6260 mL | 15.2520 mL | |
| 10 mM | 0.7626 mL | 3.8130 mL | 7.6260 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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