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| Other Sizes |
| Targets |
L-Histidine hydrochloride hydrate targets histamine receptors and metabolic pathways. Histamine, derived from L-histidine, is involved in local immune responses, regulating physiological function in the gut and as a neurotransmitter. L-Histidine is also a precursor for other bioactive molecules. Its "target" in research is histamine synthesis and histaminergic signaling.
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| ln Vitro |
In vitro, L-Histidine hydrochloride hydrate is used in cell culture as a nutrient and to study histamine production. It is a precursor for histamine via histidine decarboxylase. Its activity is studied in immune cells, neuronal cells, and other cell types to investigate histamine synthesis and its effects on cellular function.
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| ln Vivo |
In vivo, L-Histidine hydrochloride hydrate is a source of L-histidine, which is converted to histamine. Histamine is involved in local immune responses, regulating physiological function in the gut and as a neurotransmitter. L-Histidine is also incorporated into proteins and is essential for growth and tissue repair.
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| Enzyme Assay |
In vitro enzyme assays with L-Histidine hydrochloride hydrate typically involve studying histidine decarboxylase, the enzyme that converts histidine to histamine. The compound is used as a substrate to measure enzyme activity. Standard assays involve incubating L-histidine with enzyme preparations and measuring histamine production by HPLC, ELISA, or radiometric methods.
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| Cell Assay |
In vitro cell culture experiments with L-Histidine hydrochloride hydrate involve supplementing cell culture media with the amino acid. Cells are cultured with L-histidine, and endpoints include assessment of histamine production, cell proliferation, and immune cell function. It evokes disparate responses from non-tumorigenic and tumorigenic cells in culture.
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| Animal Protocol |
In vivo animal experiments with L-Histidine hydrochloride hydrate typically involve administering the compound to animals to study histamine metabolism, immune function, or nutritional requirements. It is also used in studies of histidine deficiency and its effects on growth and development. Key endpoints include histamine levels, immune responses, and tissue histopathology.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of L-Histidine hydrochloride hydrate reflect the behavior of L-histidine. L-histidine is absorbed from the gastrointestinal tract via amino acid transporters, distributed throughout the body, and utilized in protein synthesis or converted to histamine. The hydrochloride hydrate form demonstrates approximately 3.7-fold higher aqueous solubility compared to L-histidine free base.
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| Toxicity/Toxicokinetics |
L-Histidine hydrochloride hydrate has a low toxicity profile as a naturally occurring amino acid. For research use, standard laboratory safety practices are sufficient. It is not considered a hazardous substance. It is soluble in water at 169.9 g/L at 20°C.
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| Additional Infomation |
See also: Ferrous Sulfate (related).
L-Histidine hydrochloride hydrate is the hydrochloride salt of the amino acid L-histidine, a histamine precursor. Histamine is involved in local immune responses and neurotransmission. The compound is used in cell culture and biochemical research. It is not a drug and has no therapeutic indications. It is available for laboratory research use only. |
| Molecular Formula |
C6H12CLN3O3
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|---|---|
| Molecular Weight |
209.63
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| Exact Mass |
209.056
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| CAS # |
5934-29-2
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| Related CAS # |
L-Histidine-d5 hydrochloride hydrate;2483831-75-8;L-Histidine-15N hydrochloride hydrate;L-Histidine-13C hydrochloride hydrate;2483735-43-7;L-Histidine-13C6,15N3,d5 hydrochloride hydrate;2483829-32-7;L-Histidine-13C6 hydrochloride hydrate;201740-88-7;L-Histidine-13C6,15N3 hydrochloride hydrate;202468-43-7;L-Histidine-15N3 hydrochloride hydrate;L-Histidine-d3 hydrochloride hydrate;L-Histidine-15N3,d5 hydrochloride hydrate
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| PubChem CID |
165377
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| Appearance |
White to off-white solid powder
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| Density |
1.485 g/cm3
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| Boiling Point |
458.9ºC at 760 mmHg
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| Melting Point |
254 °C (dec.)(lit.)
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| Flash Point |
231.3ºC
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| LogP |
0.802
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
13
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| Complexity |
151
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C1=C(NC=N1)C[C@@H](C(=O)O)N.O.Cl
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| InChi Key |
CMXXUDSWGMGYLZ-XRIGFGBMSA-N
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| InChi Code |
InChI=1S/C6H9N3O2.ClH.H2O/c7-5(6(10)11)1-4-2-8-3-9-4;;/h2-3,5H,1,7H2,(H,8,9)(H,10,11);1H;1H2/t5-;;/m0../s1
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| Chemical Name |
(2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid;hydrate;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 100 mg/mL (477.03 mM)
DMSO: 1 mg/mL (4.77 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (238.52 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7703 mL | 23.8515 mL | 47.7031 mL | |
| 5 mM | 0.9541 mL | 4.7703 mL | 9.5406 mL | |
| 10 mM | 0.4770 mL | 2.3852 mL | 4.7703 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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