| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
D-Methionine sulfoxide targets methionine sulfoxide reductases (Msrs), enzymes that catalyze the reduction of methionine sulfoxide back to methionine. It is a substrate for these enzymes and is used to study cellular redox homeostasis. Its "target" is the methionine sulfoxide reductase system and oxidative stress pathways.
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|---|---|
| ln Vitro |
One of the most restrictive amino acids in poultry diets is methionine[1].
In vitro, D-Methionine sulfoxide is used to study oxidative stress and methionine reductase activity. It serves as a stereochemical standard for protein oxidation studies. Its activity is measured by its reduction by methionine sulfoxide reductases, which can be monitored by chromatographic or enzymatic assays. |
| ln Vivo |
In vivo, D-Methionine sulfoxide is studied in the context of oxidative stress and antioxidant defense. Methionine oxidation and reduction are important for cellular redox homeostasis. It is used to investigate the role of methionine sulfoxide reductases in protecting cells from oxidative damage.
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| Enzyme Assay |
In vitro enzyme assays with D-Methionine sulfoxide typically involve studying methionine sulfoxide reductase (Msr) activity. The compound is used as a substrate, and the reduction to methionine is measured. Standard assays involve incubating D-methionine sulfoxide with enzyme preparations in the presence of reducing equivalents (e.g., dithiothreitol or thioredoxin), and measuring methionine production by HPLC or mass spectrometry.
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| Cell Assay |
In vitro cell culture experiments with D-Methionine sulfoxide involve treating cells with the compound to induce oxidative stress or to study methionine sulfoxide reductase activity. Cells are treated with various concentrations, and endpoints include assessment of cell viability, oxidative stress markers, and methionine sulfoxide reductase activity. These experiments characterize the cellular response to oxidative stress.
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| Animal Protocol |
In vivo animal experiments with D-Methionine sulfoxide are not commonly performed as the compound is a research chemical. If used, it would be to study the effects of oxidative stress on methionine metabolism or to investigate the role of methionine sulfoxide reductases in disease models. Its primary significance is in research as a tool for studying oxidative stress and protein oxidation.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of D-Methionine sulfoxide are not well characterized. As a small, polar amino acid derivative, it is expected to be absorbed and distributed throughout the body. It may be reduced by methionine sulfoxide reductases or metabolized through other pathways. Specific PK data are not available.
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| Toxicity/Toxicokinetics |
D-Methionine sulfoxide has a low toxicity profile as an amino acid derivative. For research use, standard laboratory safety practices are sufficient. It is not considered a hazardous substance. It is a standard for studying protein oxidation and oxidative stress.
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| References | |
| Additional Infomation |
D-methionine S-oxide is a type of methionine S-oxide.
D-Methionine sulfoxide is an oxidized amino acid derivative used in research on oxidative stress and methionine reductase activity. It serves as a stereochemical standard for protein oxidation studies. The compound is not a drug and has no therapeutic indications. It is available for laboratory research use only. |
| Molecular Formula |
C5H11NO3S
|
|---|---|
| Molecular Weight |
165.21
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| Exact Mass |
165.045
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| CAS # |
21056-56-4
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| Related CAS # |
D-Methionine sulfoxide hydrochloride
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| PubChem CID |
148508
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
434.3±40.0 °C at 760 mmHg
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| Flash Point |
216.4±27.3 °C
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| Vapour Pressure |
0.0±2.2 mmHg at 25°C
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| Index of Refraction |
1.570
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| LogP |
-1.62
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
10
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| Complexity |
148
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CS(=O)CC[C@H](C(=O)O)N
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| InChi Key |
QEFRNWWLZKMPFJ-CQIZIWTCSA-N
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| InChi Code |
InChI=1S/C5H11NO3S/c1-10(9)3-2-4(6)5(7)8/h4H,2-3,6H2,1H3,(H,7,8)/t4-,10?/m1/s1
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| Chemical Name |
(2R)-2-amino-4-methylsulfinylbutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 125 mg/mL (756.61 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (302.65 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.0529 mL | 30.2645 mL | 60.5290 mL | |
| 5 mM | 1.2106 mL | 6.0529 mL | 12.1058 mL | |
| 10 mM | 0.6053 mL | 3.0265 mL | 6.0529 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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