| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
L-Isoleucine-1-13C has no independent pharmacological target. The unlabeled L-isoleucine is an essential amino acid that acts as a potent activator of the mTOR signaling pathway, which is a master regulator of cell growth and protein synthesis. It also plays a role in glucose uptake, insulin sensitivity, and immune function. As a branched-chain amino acid (BCAA), it is a key substrate for energy production in muscle tissue.
|
|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
L-Isoleucine-1-13C is used as a metabolic tracer, not to assess activity. The unlabeled L-isoleucine is a key nutrient that directly stimulates muscle protein synthesis and promotes the absorption of glucose in skeletal muscle. It has been shown to have anti-inflammatory effects in some models, helping to regulate the balance of immune cells. These functions are critical for muscle repair and metabolic health. |
| ln Vivo |
In vivo, L-isoleucine is an essential amino acid that is crucial for muscle repair, recovery, and metabolic regulation. The 13C-labeled version allows researchers to study these whole-body processes. Unlabeled L-isoleucine has been shown to improve insulin sensitivity, reduce blood glucose levels, and promote wound healing in animal models. It is also being studied for its potential to improve athletic performance and prevent muscle wasting.
|
| Enzyme Assay |
L-Isoleucine-1-13C serves as an internal standard for in vitro assays. A stock solution is typically prepared in a solvent like 0.1M HCl or water. This standard is then spiked into biological samples (e.g., plasma, tissue homogenates) before any processing. After protein precipitation and centrifugation, the sample is analyzed by LC-MS. The ratio of the unlabeled isoleucine to the L-Isoleucine-1-13C standard is used for precise quantification.
|
| Cell Assay |
For in vitro cellular experiments, L-Isoleucine-1-13C can be added to cell culture media as a metabolic tracer. For example, muscle cell lines (e.g., C2C12 myotubes) can be cultured in media containing the labeled isoleucine. After a period of incubation, the cells are harvested and the proteins are extracted. The protein is then hydrolyzed, and the resulting amino acids are analyzed by LC-MS. The amount of 13C-isoleucine incorporated into the new protein provides a direct measure of protein synthesis rates.
|
| Animal Protocol |
For in vivo animal experiments, the labeled isoleucine is typically administered to rodents via a single intravenous (IV) bolus injection or a primed, continuous infusion. Blood samples are collected from the tail vein at predetermined time points. The plasma is then analyzed by mass spectrometry to determine the enrichment of the 13C label. This data is used in kinetic models to calculate whole-body rates of protein synthesis and breakdown.
|
| ADME/Pharmacokinetics |
As a stable isotope tracer, L-Isoleucine-1-13C has the same pharmacokinetics as natural L-isoleucine. L-isoleucine is a large neutral amino acid that is rapidly absorbed from the gut. It crosses the blood-brain barrier via specific transporters. Its plasma half-life is relatively short, often under 2 hours, as it is rapidly taken up by tissues, primarily muscle, for protein synthesis or metabolism. It is an essential amino acid, so it is not synthesized endogenously.
|
| Toxicity/Toxicokinetics |
L-Isoleucine is a natural, essential amino acid with very low toxicity. It is a normal component of dietary protein and is generally recognized as safe (GRAS). The 13C-labeled version is chemically identical and poses no additional toxic risk. Even at significantly elevated levels, the body has effective mechanisms to metabolize excess BCAAs. However, chronic high levels of BCAAs are being studied for their potential link to insulin resistance.
|
| References | |
| Additional Infomation |
L-Isoleucine-1-13C is not a drug but a research-use stable isotope-labeled compound. It is used extensively in metabolic research, particularly in studies of protein turnover, energy metabolism, and diabetes. It serves as a precise internal standard for the accurate quantification of isoleucine in clinical and biological samples. It is a critical tool for understanding the role of branched-chain amino acids in health and disease, particularly in muscle wasting and insulin resistance.
|
| Molecular Formula |
C6H13NO2
|
|---|---|
| Molecular Weight |
131.17
|
| Exact Mass |
132.097
|
| CAS # |
81202-01-9
|
| Related CAS # |
L-Isoleucine;73-32-5
|
| PubChem CID |
13000968
|
| Appearance |
White to off-white solid powder
|
| LogP |
-1.7
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
9
|
| Complexity |
103
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
C(O)(=O)[C@H]([C@@H](C)CC)N
|
| InChi Key |
AGPKZVBTJJNPAG-XDXFPRKMSA-N
|
| InChi Code |
InChI=1S/C6H13NO2/c1-3-4(2)5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t4-,5-/m0/s1/i6+1
|
| Chemical Name |
(2S,3S)-2-amino-3-methyl(113C)pentanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 12.5 mg/mL (94.58 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (189.15 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.6237 mL | 38.1185 mL | 76.2369 mL | |
| 5 mM | 1.5247 mL | 7.6237 mL | 15.2474 mL | |
| 10 mM | 0.7624 mL | 3.8118 mL | 7.6237 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
