| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
D-Biotinol targets biotin-dependent enzymes, including carboxylases such as pyruvate carboxylase, acetyl-CoA carboxylase, propionyl-CoA carboxylase, and β-methylcrotonyl-CoA carboxylase. As a structural analog of biotin, it can participate in carboxylase-related reactions and serve as a coenzyme structural analog.
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| ln Vitro |
In vitro, D-biotinol is used to study the reaction mechanisms of biotin-dependent enzymes. It serves as a tool molecule to investigate the role of biotin in carboxylation reactions and to elucidate the catalytic mechanisms of carboxylases. The compound's structural similarity to biotin allows it to mimic biotin's interactions with enzymes while providing distinct chemical properties for research purposes.
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| ln Vivo |
In vivo, D-biotinol has been shown to restore biotin deficiency induced by egg whites in rats. It is orally bioactive and can be converted into biotin by rats, making it a useful compound for studying biotin metabolism and deficiency disorders. Its ability to replace biotin in vivo highlights its potential as a biotin supplement or prodrug.
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| Enzyme Assay |
In vitro enzyme assays for D-biotinol typically measure the activity of biotin-dependent carboxylases in the presence of the compound. D-Biotinol is used as a substrate analog to study the binding and catalytic mechanisms of these enzymes. The assays involve incubating the enzyme with D-biotinol and other substrates, and the reaction products are quantified by spectrophotometric or chromatographic methods to assess enzyme activity.
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| Cell Assay |
In vitro cell experiments with D-biotinol involve culturing cells in biotin-deficient media supplemented with the compound to study its ability to support biotin-dependent cellular processes. Cells are assessed for growth, proliferation, and metabolic activity. The compound is also used to study biotin transport and metabolism in cell culture models of biotin deficiency.
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| Animal Protocol |
In vivo animal experiments with D-biotinol involve inducing biotin deficiency in rats by feeding them raw egg whites, which contain avidin that binds biotin. D-Biotinol is then administered orally to the deficient animals, and the restoration of biotin-dependent functions is assessed. This model is used to study the bioavailability and efficacy of D-biotinol as a biotin replacement.
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| ADME/Pharmacokinetics |
D-Biotinol is orally bioactive and can be converted into biotin by rats. It is a water-soluble small molecule containing a sulfur-containing heterocycle. The compound's structural similarity to biotin allows it to be recognized by biotin transporters and metabolized to biotin. It is typically stored as a solid at room temperature.
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| Toxicity/Toxicokinetics |
Toxicological data for D-biotinol are limited. As a reduced derivative of biotin, it is expected to have low toxicity similar to biotin, which is a water-soluble vitamin with a high safety margin. The compound is intended for research use only and not for human consumption.
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| References | |
| Additional Infomation |
D-Biotinol is a research compound with applications in enzymology, biotin metabolism, and chemical biology. It is commonly used to study the reaction mechanisms of biotin-dependent enzymes or as a tool molecule in research on chemical modification and labeling. No clinical trials or approved therapeutic indications exist for this compound. Its mechanism of action involves participation in carboxylase-related reactions as a coenzyme structural analog.
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| Molecular Formula |
C10H18N2O2S
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|---|---|
| Molecular Weight |
230.33
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| Exact Mass |
230.109
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| CAS # |
53906-36-8
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| PubChem CID |
10082587
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| Appearance |
White to off-white solid powder
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| LogP |
1.362
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
15
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| Complexity |
238
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1[C@H]2[C@@H]([C@@H](S1)CCCCCO)NC(=O)N2
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| InChi Key |
RGIKRHKHRAAZIO-CIUDSAMLSA-N
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| InChi Code |
InChI=1S/C10H18N2O2S/c13-5-3-1-2-4-8-9-7(6-15-8)11-10(14)12-9/h7-9,13H,1-6H2,(H2,11,12,14)/t7-,8-,9-/m0/s1
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| Chemical Name |
(3aS,4S,6aR)-4-(5-hydroxypentyl)-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (434.16 mM)
Methanol: 25 mg/mL (108.54 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.85 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3416 mL | 21.7080 mL | 43.4160 mL | |
| 5 mM | 0.8683 mL | 4.3416 mL | 8.6832 mL | |
| 10 mM | 0.4342 mL | 2.1708 mL | 4.3416 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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