| Size | Price | Stock | Qty |
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| 1g |
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| 5g |
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| Other Sizes |
| Targets |
12-Hydroxydodecanoic acid targets lipid metabolism pathways as a metabolite of lauric acid. It is involved in fatty acid oxidation and may interact with enzymes of the cytochrome P450 family that hydroxylate fatty acids. It also serves as a precursor in the synthesis of polyesters and other polymeric materials.
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| ln Vitro |
In vitro, 12-hydroxydodecanoic acid is used in studies of lipid metabolism and fatty acid oxidation. It serves as a substrate for enzymes involved in the beta-oxidation pathway and as a building block for the synthesis of biodegradable polymers. Researchers also use this compound to investigate the structural and functional characterization of enzymes such as S-nitrosoglutathione reductase in tomato plants.
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| ln Vivo |
In vivo, 12-hydroxydodecanoic acid is a metabolite of lauric acid, a medium-chain fatty acid found in coconut oil and other dietary sources. It is metabolized through beta-oxidation and may be further oxidized to dicarboxylic acids. Its role in lipid metabolism makes it a subject of study in metabolic disorders and nutritional research.
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| Enzyme Assay |
In vitro enzyme assays for 12-hydroxydodecanoic acid typically involve studying fatty acid hydroxylases and oxidases. The compound is used as a substrate to measure the activity of cytochrome P450 enzymes that hydroxylate fatty acids at the terminal position. Assays are performed using isolated enzymes or microsomal preparations, and the product formation is monitored by chromatographic methods such as HPLC or GC-MS.
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| Cell Assay |
In vitro cell experiments with 12-hydroxydodecanoic acid involve treating cell lines, such as hepatocytes or adipocytes, to study fatty acid metabolism. Cells are cultured with the compound, and its effects on lipid accumulation, gene expression of metabolic enzymes, and fatty acid oxidation are assessed. The compound may also be used to study the cellular uptake and metabolism of hydroxylated fatty acids.
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| Animal Protocol |
In vivo animal experiments using 12-hydroxydodecanoic acid are not extensively documented. Related studies on lauric acid metabolism may involve administering the compound or its precursors to animal models and measuring the levels of 12-hydroxydodecanoic acid and other metabolites in plasma, urine, or tissues using chromatographic and mass spectrometric techniques.
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
12-Hydroxylauric acid is a known metabolite of lauric acid in the human body. Pharmacokinetic data for 12-hydroxydodecanoic acid are limited. As a fatty acid derivative, it is expected to be absorbed through dietary intake and metabolized via beta-oxidation. It may undergo further oxidation to dicarboxylic acids or conjugation reactions. The compound has a melting point of 85.00 to 88.00 °C and is a solid at room temperature. |
| Toxicity/Toxicokinetics |
12-Hydroxylauric acid is a known human metabolite and is generally considered to have low toxicity. However, comprehensive toxicological data are not available. As a fatty acid derivative, it is expected to be well-tolerated at physiological concentrations. High doses may potentially affect lipid metabolism, but specific toxicity studies have not been reported.
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| Additional Infomation |
12-Hydroxylauric acid (12-hydroxylauric acid) is a medium-chain fatty acid and a 12-hydroxy derivative of lauric acid. It is a human metabolite functionally related to dodecanoic acid and is the conjugate acid of 12-hydroxylaurate. 12-Hydroxylauric acid has been reported to be detected in Trypanosoma brevicornu and Pinus radiata, and relevant data are available for reference.
12-Hydroxydodecanoic acid is a research chemical with applications in polymer chemistry, lipid metabolism studies, and material science. It is used as a PROTAC linker in the synthesis of proteolysis-targeting chimeras. No clinical trials or approved therapeutic indications exist for this compound. Its mechanism of action in biological systems is related to its role as a fatty acid metabolite and a building block for biodegradable polymers. |
| Molecular Formula |
C12H24O3
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|---|---|
| Molecular Weight |
216.32
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| Exact Mass |
216.173
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| CAS # |
505-95-3
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| Related CAS # |
27925-01-5
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| PubChem CID |
79034
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| Appearance |
White to off-white solid powder
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| Density |
0.988g/cm3
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| Boiling Point |
359.2ºC at 760 mmHg
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| Melting Point |
85.00 to 88.00 °C. @ 760.00 mm Hg
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| Flash Point |
185.2ºC
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| Index of Refraction |
1.467
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| LogP |
2.964
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
11
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| Heavy Atom Count |
15
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| Complexity |
146
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(CCCCCC(=O)O)CCCCCO
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| InChi Key |
ZDHCZVWCTKTBRY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H24O3/c13-11-9-7-5-3-1-2-4-6-8-10-12(14)15/h13H,1-11H2,(H,14,15)
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| Chemical Name |
12-hydroxydodecanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (462.28 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.56 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.56 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6228 mL | 23.1139 mL | 46.2278 mL | |
| 5 mM | 0.9246 mL | 4.6228 mL | 9.2456 mL | |
| 10 mM | 0.4623 mL | 2.3114 mL | 4.6228 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Link: https://clinicaltrials.gov/ct2/show/NCT02408185
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