| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
L-Aspartic acid-13C has no pharmacological target, as it is a stable isotope tracer. The unlabeled L-aspartic acid is a naturally occurring amino acid that acts as an excitatory neurotransmitter in the central nervous system (CNS) by binding to and activating NMDA, AMPA, and kainate receptors. It also plays a fundamental role in the urea cycle, gluconeogenesis, and the synthesis of other amino acids and nucleotides.
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| ln Vitro |
It has been demonstrated that L-aspartic acid is an appropriate prodrug for colon-specific drug delivery[1]. Preadministration of 100 mM L-Aspartic acid and 100 mM L-Glu increases the amount of L-[3H]Asp that is still in the brain at 5 minutes by 206 and 178%, respectively; L-[3H]Asp efflux transport is unaffected by 100 mM D-Asp. The coadministration of 100 mM L-Aspartic acid and 100 mM L-Glu, respectively, increases that value for L-[3H]Glu at 20 minutes by 145 and 156%, but not by D-Asp. It's noteworthy to note that L-Aspartic acid appears to traverse the BBB seven times faster than L-Glu does[2].
As an isotope tracer, L-Aspartic acid-13C is not tested for pharmacological activity. The unlabeled L-aspartic acid has several in vitro activities: it is a key substrate in the malate-aspartate shuttle, crucial for transferring reducing equivalents across the mitochondrial membrane. It is also a precursor to asparagine, and it plays a role in the synthesis of purines and pyrimidines, the building blocks of DNA and RNA. |
| ln Vivo |
In vivo, L-aspartic acid is a critical component of many metabolic pathways. The 13C-labeled version is used to trace these pathways. The unlabeled L-aspartic acid is known to stimulate the NMDA receptor, which is involved in learning, memory, and synaptic plasticity. It is also being researched for its role in athletic performance, anti-fatigue effects, and as a potential dietary supplement.
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| Enzyme Assay |
L-Aspartic acid-13C is used as an internal standard in LC-MS or GC-MS. For in vitro assays, it is dissolved in a suitable solvent (e.g., 0.1% formic acid in water) to prepare a stock solution. A known amount of this solution is spiked into biological samples (e.g., plasma, tissue homogenates) before sample preparation. The samples are then processed and analyzed by mass spectrometry, and the 13C-labeled standard allows for precise quantification of the endogenous L-aspartic acid.
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| Cell Assay |
For in vitro cell culture experiments, L-Aspartic acid-13C is used as a metabolic tracer. Cells are cultured in a medium containing the 13C-labeled aspartic acid. At specific time points, the cells are harvested and the intracellular metabolites are extracted. The extracted samples are analyzed by LC-MS or NMR to determine how the 13C label has been incorporated into downstream metabolites like arginine, asparagine, and intermediates of the TCA cycle, providing insights into cellular metabolic flux.
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| Animal Protocol |
For in vivo animal experiments, L-Aspartic acid-13C is administered to rodents, typically via an intravenous injection or oral gavage. Blood and tissue samples are collected at predetermined time points. The samples are then processed and analyzed by LC-MS to track the distribution and metabolism of the labeled aspartic acid. This allows researchers to study whole-body nitrogen and carbon metabolism, particularly in organs like the liver and kidneys where this amino acid is central to key metabolic pathways.
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| ADME/Pharmacokinetics |
L-Aspartic acid-13C is a tracer and does not have separate pharmacokinetic (PK) parameters of its own. Its properties are identical to those of natural L-aspartic acid. L-aspartic acid is rapidly absorbed from the gut and distributed throughout the body. It is a non-essential amino acid, meaning the body can synthesize it, so its half-life can be influenced by de novo synthesis. It is primarily metabolized in the liver and kidneys and is not significantly stored in the body.
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| Toxicity/Toxicokinetics |
L-aspartic acid is a naturally occurring, low-toxicity amino acid. It is generally recognized as safe (GRAS) as a nutrient. The 13C-labeled version is chemically identical and poses no additional toxicity risk. In research settings, it is used in very small, non-toxic amounts to trace metabolic pathways, well below any levels that would cause adverse effects from the amino acid itself.
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| References |
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| Additional Infomation |
L-Aspartic acid-13C is not a drug but a stable isotope-labeled research chemical. It is used for metabolic flux analysis to study the tricarboxylic acid (TCA) cycle, gluconeogenesis, and the urea cycle. It also serves as a crucial internal standard in metabolomics and clinical diagnostic research for the precise quantification of L-aspartic acid levels in complex biological fluids like blood, urine, and cerebrospinal fluid.
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| Molecular Formula |
C313CH7NO4
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|---|---|
| Molecular Weight |
134.10
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| Exact Mass |
134.04
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| CAS # |
81201-97-0
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| Related CAS # |
L-Aspartic acid;56-84-8
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| PubChem CID |
16213451
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Melting Point |
>300ºC (dec.)(lit.)
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| Index of Refraction |
1.531
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| LogP |
-2.8
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
9
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| Complexity |
133
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[C@@H](N)([13C](=O)O)CC(=O)O
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| InChi Key |
CKLJMWTZIZZHCS-GZPBOPPUSA-N
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| InChi Code |
InChI=1S/C4H7NO4/c5-2(4(8)9)1-3(6)7/h2H,1,5H2,(H,6,7)(H,8,9)/t2-/m0/s1/i4+1
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| Chemical Name |
(2S)-2-amino(113C)butanedioic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 5 mg/mL (37.29 mM)
DMSO: < 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2 mg/mL (14.91 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.4571 mL | 37.2856 mL | 74.5712 mL | |
| 5 mM | 1.4914 mL | 7.4571 mL | 14.9142 mL | |
| 10 mM | 0.7457 mL | 3.7286 mL | 7.4571 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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