| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
L-Serine-d3 has no independent pharmacological target as a stable isotope tracer. The unlabeled L-serine is a non-essential amino acid that plays a critical role in the biosynthesis of other amino acids (glycine, cysteine), phospholipids (phosphatidylserine, sphingolipids), and nucleotides. L-Serine is also a key metabolite in one-carbon metabolism and is involved in the folate cycle.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a stable isotope tracer, L-Serine-d3 is not tested for in vitro pharmacological activity. In cell culture studies, it is used to trace serine metabolism, one-carbon metabolism, and glycine and nucleotide biosynthesis. L-Serine is an essential nutrient for the growth and survival of many cancer cell lines, and the labeled version enables precise tracking of its metabolic fate. |
| ln Vivo |
L-Serine-d3 has no independent in vivo pharmacological activity as a therapeutic agent. It is used in animal and human studies as a stable isotope tracer to measure whole-body serine metabolism and one-carbon flux. L-Serine supplementation has been investigated for its potential therapeutic effects in neurological disorders, including amyotrophic lateral sclerosis (ALS) and hereditary sensory and autonomic neuropathy type 1 (HSAN1).
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| Enzyme Assay |
For in vitro LC-MS/MS quantification, L-Serine-d3 is dissolved in an appropriate solvent (water, 0.1% formic acid, or methanol) to prepare a stock solution (e.g., 1 mg/mL). The internal standard is added to biological samples (plasma, serum, cell lysates) at a fixed concentration (e.g., 10-500 ng/mL). Protein precipitation is performed by adding 3-5 volumes of methanol or acetonitrile containing the internal standard. After centrifugation, the supernatant is analyzed by LC-MS/MS.
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| Cell Assay |
For in vitro cellular experiments, L-Serine-d3 can be added to cell culture media as a tracer. Cells (e.g., cancer cells, primary neurons) are cultured in standard medium. The medium is replaced with serine-free medium containing L-Serine-d3 (10-500 uM). After 1-48 hours, cells are harvested, and metabolites are extracted. LC-MS or GC-MS is used to trace the deuterium label into glycine, formate, nucleotides, and other one-carbon metabolites.
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| Animal Protocol |
For in vivo animal studies, L-Serine-d3 is administered to rodents via intravenous injection, intraperitoneal injection (50-200 mg/kg), or oral gavage. Blood samples are collected at multiple time points (0, 15, 30, 60, 120, 240 minutes). At terminal time points, tissues (liver, brain, kidney) are harvested, snap-frozen, and stored at -80degC. Tissues are homogenized and analyzed by LC-MS to quantify 2H-labeled serine and its metabolites.
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| ADME/Pharmacokinetics |
L-Serine-d3 is a stable isotope tracer and has the same pharmacokinetics as natural L-serine. L-Serine is a small, hydrophilic amino acid with rapid absorption (Tmax ∼30-60 min), distribution to all tissues, and the ability to cross the blood-brain barrier via the large neutral amino acid transporter. Plasma half-life is approximately 30-90 minutes. L-Serine is metabolized primarily in the liver via serine hydroxymethyltransferase (SHMT) to glycine, donating one-carbon units.
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| Toxicity/Toxicokinetics |
L-Serine is a naturally occurring amino acid with very low toxicity (GRAS status). The LD50 in rodents is >5,000 mg/kg. The deuterated version is chemically identical and has the same safety profile. Standard laboratory safety precautions for handling amino acids apply. Not intended for human consumption. High-dose L-serine supplementation (0.5-2 g/kg/day) is generally well-tolerated in animal studies.
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| References | |
| Additional Infomation |
L-Serine-d3 is not a drug but a stable isotope-labeled research tracer. It has no approved therapeutic status. It is used as an internal standard for LC-MS quantification of L-serine in biological samples and as a tracer for metabolic studies of one-carbon metabolism, nucleotide biosynthesis, and cancer metabolism. It is also used in research on serine deficiency disorders (e.g., Neu-Laxova syndrome) and neurological diseases (ALS, HSAN1). Available with ≥98% atom% D.
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| Molecular Formula |
C3H4D3NO3
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|---|---|
| Molecular Weight |
108.11
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| Exact Mass |
108.061
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| CAS # |
105591-10-4
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| Related CAS # |
L-Serine;56-45-1
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| PubChem CID |
54089883
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
394.8±32.0 °C at 760 mmHg
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| Flash Point |
192.6±25.1 °C
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| Vapour Pressure |
0.0±2.1 mmHg at 25°C
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| Index of Refraction |
1.519
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| LogP |
-1.58
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
7
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| Complexity |
72.6
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[2H][C@@](C(=O)O)(C([2H])([2H])O)N
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| InChi Key |
MTCFGRXMJLQNBG-RBXBQAPRSA-N
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| InChi Code |
InChI=1S/C3H7NO3/c4-2(1-5)3(6)7/h2,5H,1,4H2,(H,6,7)/t2-/m0/s1/i1D2,2D
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| Chemical Name |
(2S)-2-amino-2,3,3-trideuterio-3-hydroxypropanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 125 mg/mL (1156.23 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (462.49 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.2498 mL | 46.2492 mL | 92.4984 mL | |
| 5 mM | 1.8500 mL | 9.2498 mL | 18.4997 mL | |
| 10 mM | 0.9250 mL | 4.6249 mL | 9.2498 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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