| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| Other Sizes |
| Targets |
The compound targets nucleoside diphosphate kinase (NDPK, EC 2.7.4.6). dCDP serves as a key substrate for this enzyme in the enzymatic synthesis of dCTP. dCTP is essential for DNA biosynthesis and reverse transcription.
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|---|---|
| ln Vitro |
In vitro, dCDP is used as a substrate for nucleoside diphosphate kinase (NDPK) to produce dCTP. The compound is essential for studies of DNA biosynthesis and reverse transcription. It is used in biochemical assays to measure NDPK activity and to study nucleotide metabolism. dCDP plays a crucial role in biochemical reactions.
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| ln Vivo |
In vivo, dCDP is an intermediate in pyrimidine nucleotide metabolism. It is converted to dCTP by nucleoside diphosphate kinase, which is essential for DNA biosynthesis and reverse transcription. The compound is involved in the regulation of nucleotide pools and DNA synthesis in cells.
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| Enzyme Assay |
In vitro enzyme assays for nucleoside diphosphate kinase involve incubating the enzyme with dCDP and ATP as phosphate donor in Tris-HCl buffer at pH 7.4, typically at 37°C. The reaction product, dCTP, is quantified by HPLC or LC-MS/MS. Enzyme activity is measured by monitoring the formation of dCTP or the consumption of ATP. Kinetic parameters are determined by varying dCDP concentrations.
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| Cell Assay |
For in vitro cell assays, cells are cultured in medium supplemented with dCDP at concentrations of 0.1-10 mM. Cellular nucleotide levels are analyzed by LC-MS/MS. The compound's effects on DNA synthesis and cell proliferation are assessed by measuring dCTP pools and thymidine incorporation. The compound is used to study nucleotide metabolism and the regulation of DNA biosynthesis.
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| Animal Protocol |
For in vivo animal studies, dCDP is administered to rodents via oral gavage or intravenous injection at doses of 10-100 mg/kg. Blood and tissue samples are collected for pharmacokinetic analysis. Nucleotide levels are quantified by LC-MS/MS. The compound is used in studies of pyrimidine metabolism and DNA biosynthesis. Tissue distribution and metabolic effects are evaluated.
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| ADME/Pharmacokinetics |
dCDP trisodium (MW ~451.1) has a molecular formula of C9H12N3Na3O10P2. It is soluble in water. The compound is stable under recommended storage conditions (powder at -20°C, in solvent at -80°C). It is a substrate for nucleoside diphosphate kinase (EC 2.7.4.6) and is used in studies of DNA biosynthesis and reverse transcription.
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| Toxicity/Toxicokinetics |
The compound is for research use only. No specific toxicity data are available. As a nucleotide metabolite, it is expected to have low toxicity at physiological concentrations. High doses may affect nucleotide metabolism and DNA synthesis. Standard safety precautions should be followed.
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| Additional Infomation |
2'-Deoxycytidine-5'-diphosphate trisodium (CAS# 151151-32-5) is a research reagent used primarily in studies of nucleotide metabolism, DNA biosynthesis, and enzymology. It has not been investigated in clinical trials nor approved as a therapeutic drug. The compound is used as a substrate for nucleoside diphosphate kinase in the production of dCTP to support DNA biosynthesis and reverse transcription.
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| Molecular Formula |
C9H12N3NA3O10P2
|
|---|---|
| Molecular Weight |
453.12
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| Exact Mass |
409.005
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| CAS # |
151151-32-5
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| PubChem CID |
71315445
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
27
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| Complexity |
640
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| Defined Atom Stereocenter Count |
3
|
| SMILES |
C1[C@@H]([C@H](O[C@H]1N2C=CC(=NC2=O)N)COP(=O)([O-])OP(=O)([O-])[O-])O.[Na+].[Na+].[Na+]
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| InChi Key |
SXMDNHAPEVRFNF-MILVPLDLSA-K
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| InChi Code |
InChI=1S/C9H15N3O10P2.3Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(21-8)4-20-24(18,19)22-23(15,16)17;;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H2,10,11,14)(H2,15,16,17);;;/q;3*+1/p-3/t5-,6+,8+;;;/m0.../s1
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| Chemical Name |
trisodium;[[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-oxidophosphoryl] phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (551.73 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (220.69 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2069 mL | 11.0346 mL | 22.0692 mL | |
| 5 mM | 0.4414 mL | 2.2069 mL | 4.4138 mL | |
| 10 mM | 0.2207 mL | 1.1035 mL | 2.2069 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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