| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Human Endogenous Metabolite
D-Arginine targets arginine decarboxylase (EC 4.1.1.19) as an inhibitor. It is the D-isomer of arginine and is considered an inactive form of L-arginine. It is an endogenous metabolite. It has broad-spectrum toxicity to microorganisms and acts as a regulator of microbial subpopulations. |
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| ln Vitro |
In vitro, D-Arginine is the D-isomer and inactive form of L-Arginine. It inhibits arginine decarboxylase (EC 4.1.1.19). It has broad-spectrum toxicity to microorganisms and acts as a regulator of microbial subpopulations. It is used as an organic substrate to study the metabolic versatility of freshwater sedimentary archaea.
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| ln Vivo |
In vivo, D-Arginine is a semi-essential amino acid that maintains cell death in the central nervous system (CNS). It may possess neuroprotective actions against glucocorticoids induced neurotoxicity in the CNS. It inhibits the antinociceptive activity of L-arginine in a mouse model of carrageenan-induced pain when administered at a dose of 1,000 mg/kg.
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| Enzyme Assay |
In vitro enzyme assays for arginine decarboxylase involve incubating the enzyme with D-Arginine as inhibitor and L-arginine as substrate in buffer at pH 7.4. The production of agmatine is quantified by HPLC or LC-MS/MS. Inhibition constants are determined from dose-response curves. The compound's effects on microbial growth are assessed by measuring optical density in bacterial cultures.
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| Cell Assay |
For in vitro cell assays, cells are cultured in medium supplemented with D-Arginine at concentrations of 0.1-10 mM. Cell viability is assessed by MTT assay. The compound's effects on arginine metabolism and nitric oxide production are evaluated by measuring arginine and citrulline levels. Neuroprotective effects are assessed in neuronal cell models treated with glucocorticoids.
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| Animal Protocol |
For in vivo animal studies, D-Arginine is administered to rodents via oral gavage or intraperitoneal injection at doses of 10-1000 mg/kg. Blood and tissue samples are collected for pharmacokinetic analysis. Antinociceptive effects are evaluated in pain models. Neuroprotective effects are assessed in models of glucocorticoid-induced neurotoxicity. Plasma arginine levels are quantified by LC-MS/MS.
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| ADME/Pharmacokinetics |
D-Arginine (MW 174.20) has a molecular formula of C6H14N4O2. It is soluble in water. The compound is stable under standard storage conditions. As the D-isomer of arginine, it is metabolized through different pathways than L-arginine. It is an EC 4.1.1.19 inhibitor and an endogenous metabolite.
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| Toxicity/Toxicokinetics |
The compound is for research use only. No specific toxicity data are available. D-Arginine has broad-spectrum toxicity to microorganisms and may have effects on microbial populations. High doses may affect arginine metabolism. Standard safety precautions should be followed.
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| References | |
| Additional Infomation |
D-arginine is a D-α-amino acid and a D-isomer of arginine. It is an EC 4.1.1.19 (arginine decarboxylase) inhibitor and a mouse metabolite. It is a D-α-amino acid, specifically arginine. It is the conjugate base of D-arginine (1+), the conjugate acid of D-arginine, and an enantiomer of L-arginine. D-α-amino acid, a D-isomer of arginine (only L-arginine has physiological activity). There are reports of the presence of D-arginine in Homo sapiens, and relevant data are available.
D-Arginine (CAS# 157-06-2) is an endogenous metabolite and research reagent used primarily in studies of arginine metabolism, enzyme inhibition, and neuroprotection. It has not been investigated in clinical trials nor approved as a therapeutic drug. The compound is the inactive D-isomer of L-arginine and is used as an inhibitor of arginine decarboxylase and in studies of microbial metabolism. |
| Molecular Formula |
C6H14N4O2
|
|---|---|
| Molecular Weight |
174.20
|
| Exact Mass |
174.111
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| CAS # |
157-06-2
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| Related CAS # |
61155-84-8
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| PubChem CID |
71070
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| Appearance |
White to yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
367.6±52.0 °C at 760 mmHg
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| Melting Point |
221-224ºC
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| Flash Point |
176.1±30.7 °C
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| Vapour Pressure |
0.0±1.8 mmHg at 25°C
|
| Index of Refraction |
1.601
|
| LogP |
-1.79
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| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
12
|
| Complexity |
176
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
C(C[C@H](C(=O)O)N)CN=C(N)N
|
| InChi Key |
ODKSFYDXXFIFQN-SCSAIBSYSA-N
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| InChi Code |
InChI=1S/C6H14N4O2/c7-4(5(11)12)2-1-3-10-6(8)9/h4H,1-3,7H2,(H,11,12)(H4,8,9,10)/t4-/m1/s1
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| Chemical Name |
(2R)-2-amino-5-(diaminomethylideneamino)pentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 25 mg/mL (143.51 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (574.05 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.7405 mL | 28.7026 mL | 57.4053 mL | |
| 5 mM | 1.1481 mL | 5.7405 mL | 11.4811 mL | |
| 10 mM | 0.5741 mL | 2.8703 mL | 5.7405 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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