| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Stearic acid-d3 targets lipid metabolism pathways as a stable isotope-labeled internal standard. It is used for the accurate quantification of stearic acid in complex biological matrices and as a tracer for elucidating the metabolic fate of fatty acids.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, stearic acid-d3 is used as an internal standard for the quantification of stearic acid by mass spectrometry and NMR spectroscopy. Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. The compound enhances detection and quantification in metabolic studies. |
| ln Vivo |
In vivo, stearic acid-d3 is used as a tracer to study lipid metabolism and the metabolic fate of fatty acids. The deuterium labeling allows researchers to track the absorption, distribution, metabolism, and excretion of stearic acid in animal models. It is an invaluable tool in pharmaceutical and biochemical research for understanding lipid metabolism and its implications for diseases.
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| Enzyme Assay |
In vitro assays using stearic acid-d3 as an internal standard involve spiking the compound into biological samples (plasma, tissue extracts) prior to extraction and LC-MS/MS or GC-MS analysis. The deuterated compound is used to correct for matrix effects and ionization efficiency variations. For metabolic studies, the compound is incubated with liver microsomes or cell lysates, and metabolites are analyzed by mass spectrometry.
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| Cell Assay |
For in vitro cell assays, cells are cultured in medium supplemented with stearic acid-d3 at concentrations of 0.1-10 mM, often complexed with BSA. Fatty acid uptake and metabolism are analyzed by LC-MS/MS. The deuterated compound serves as an internal standard for quantifying stearic acid levels in cells. Lipid metabolism and fatty acid oxidation are assessed by measuring labeled and unlabeled fatty acid species.
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| Animal Protocol |
For in vivo animal studies, stearic acid-d3 is administered to rodents via oral gavage or intravenous injection at doses of 10-100 mg/kg. Blood and tissue samples are collected for pharmacokinetic analysis. Stearic acid levels are quantified by LC-MS/MS using the deuterated compound as an internal standard. Tissue distribution and metabolic profiling are performed to understand fatty acid metabolism.
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| ADME/Pharmacokinetics |
Stearic acid-d3 (MW 287.50 g/mol) has a chemical formula of C18H33D3O2. It is a high-purity compound (99.9%). The deuterium labeling provides a mass shift of +3 Da for MS detection. The compound is stable under standard storage conditions. As a fatty acid, it is metabolized through β-oxidation. It is a long-chain dietary saturated fatty acid.
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| Toxicity/Toxicokinetics |
The compound is for research use only. No specific toxicity data are available. Stearic acid is generally recognized as safe at dietary levels. The deuterated form is not expected to exhibit additional toxicity. Standard safety precautions should be followed.
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| References | |
| Additional Infomation |
Stearic acid (d3) is a long-chain fatty acid and a deuterated fatty acid.
Stearic acid-d3 (CAS# 62163-39-7) is a stable isotope-labeled compound used primarily as an internal standard for the quantification of stearic acid by mass spectrometry and NMR. It has not been investigated in clinical trials nor approved as a therapeutic drug. The compound is widely used in lipidomics, metabolic research, and pharmaceutical development. It serves as a powerful tracer for elucidating the metabolic fate of fatty acids. |
| Molecular Formula |
C18H33D3O2
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|---|---|
| Molecular Weight |
287.50
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| Exact Mass |
287.29
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| CAS # |
62163-39-7
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| Related CAS # |
Stearic acid;57-11-4
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| PubChem CID |
16213464
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| Appearance |
White to off-white solid powder
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| Density |
0.897g/cm3
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| Boiling Point |
361ºC(lit.)
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| Melting Point |
68-70ºC(lit.)
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| Flash Point |
162.4ºC
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| Index of Refraction |
1.455
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| LogP |
6.332
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
16
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| Heavy Atom Count |
20
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| Complexity |
202
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])CCCCCCCCCCCCCCCCC(=O)O
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| InChi Key |
QIQXTHQIDYTFRH-FIBGUPNXSA-N
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| InChi Code |
InChI=1S/C18H36O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18(19)20/h2-17H2,1H3,(H,19,20)/i1D3
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| Chemical Name |
18,18,18-trideuteriooctadecanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 12.5 mg/mL (43.48 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (4.35 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4783 mL | 17.3913 mL | 34.7826 mL | |
| 5 mM | 0.6957 mL | 3.4783 mL | 6.9565 mL | |
| 10 mM | 0.3478 mL | 1.7391 mL | 3.4783 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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