| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
Testosterone sulfate has no direct pharmacological target; its primary role is as an inert, circulating reservoir of testosterone. Testosterone sulfate is an endogenous metabolite and a conjugate of testosterone. Its primary biological purpose is to serve as an inactive, water-soluble form of testosterone, acting as a circulating reservoir. It can be converted back to the active testosterone in peripheral tissues via the action of steroid sulfatase, making it relevant in the study of androgen metabolism and endocrinology. |
|---|---|
| ln Vitro |
In vitro, testosterone sulfate is generally considered an inactive metabolite, used primarily as a reference standard. While testosterone itself is a potent androgen receptor (AR) agonist, its sulfate conjugate has a very low affinity for the androgen receptor. Research focuses on the role of steroid sulfatase in converting this conjugate back to the active free steroid, making it a key player in the intracrine regulation of androgenic activity in tissues like the prostate, skin, and brain.
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| ln Vivo |
In vivo, testosterone sulfate is a naturally occurring circulating metabolite. It is formed in the liver and other tissues through the sulfation of testosterone by sulfotransferase enzymes (SULTs). It represents a major fraction of androgens in the bloodstream, serving as a stable and inert reservoir. It can be taken up by peripheral tissues and reactivated by the enzyme steroid sulfatase, contributing to tissue-specific androgenic or estrogenic effects. This pathway is a key target for research into conditions like prostate cancer and endometriosis.
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| Enzyme Assay |
Testosterone sulfate (pyridinium) is used as an analytical standard for non-cellular assays. For LC-MS method development, it is dissolved in an organic solvent like methanol to prepare a stock solution (e.g., 1 mg/mL). This solution is then diluted to prepare a calibration curve. It is also spiked into biological samples (e.g., plasma, serum, urine) as an internal standard to ensure accurate quantification of the endogenous testosterone sulfate by correcting for matrix effects and sample recovery.
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| Cell Assay |
Testosterone sulfate (pyridinium) is not used as a direct treatment in cell-based studies. It is used as an analytical standard. For example, to measure the activity of steroid sulfatase, cells (e.g., JEG-3 choriocarcinoma cells) are treated with a steroid sulfate substrate (often [3H]-DHEAS). The reaction product is then quantified. The unlabeled testosterone sulfate standard would be used to create a calibration curve for LC-MS to precisely measure the amount of product (e.g., testosterone) formed from the conjugate.
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| Animal Protocol |
Testosterone sulfate (pyridinium) is not typically administered to animals as a treatment. It is used as an analytical standard to measure endogenous levels in biological samples from animal studies. For example, in a rat model of a metabolic disease, plasma samples are collected. The pyridinium salt is used to create calibration curves. This allows researchers to accurately quantify the levels of this metabolite in the circulation, which can serve as a biomarker of androgen status or activity.
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| ADME/Pharmacokinetics |
Testosterone sulfate is the sodium salt of a naturally occurring steroid conjugate. The elimination half-life of testosterone sulfate in humans is longer than that of free testosterone, as it is more stable and less readily metabolized. It is primarily cleared by the kidneys, and its concentration in serum is a balance between production via sulfation in the liver and clearance by excretion and desulfation in target tissues. The pyridinium salt form has similar pharmacokinetic properties.
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| Toxicity/Toxicokinetics |
Testosterone is the primary male sex hormone, and its sulfate conjugate is a normal, non-toxic metabolite. The compound is generally recognized as safe for research purposes. However, as with any research chemical, standard safety precautions should be taken, including the use of gloves and protective clothing. It should be stored at -20degC as a powder and protected from moisture and light. It is not intended for human consumption.
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| References |
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| Additional Infomation |
Testosterone sulfate (pyridinium) is not a drug but a research-use analytical standard. It is used as an internal standard or reference material for the quantification of testosterone sulfate in biological matrices (blood, urine, tissues). Its primary value is in the fields of endocrinology, steroid biochemistry, and drug metabolism. Researchers use it to study steroid hormone metabolism, the enterohepatic circulation of steroids, and the activity of steroid sulfatase and sulfotransferase enzymes.
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| Molecular Formula |
C24H33NO5S
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|---|---|
| Molecular Weight |
447.59
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| Exact Mass |
447.208
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| CAS # |
34991-10-1
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| Related CAS # |
Testosterone sulfate;651-45-6
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| PubChem CID |
172878669
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
31
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| Complexity |
752
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| Defined Atom Stereocenter Count |
6
|
| SMILES |
O=C1C=C2[C@]([C@@]3([C@@]([H])(CC2)[C@@]2([H])[C@]([C@H](CC2)OS(=O)(O)=O)(C)CC3)[H])(C)CC1.C1=CC=CC=N1
|
| InChi Key |
UBMZFWYWFPIOIG-JZSNIJFVSA-N
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| InChi Code |
InChI=1S/C19H28O5S.C5H5N/c1-18-9-7-13(20)11-12(18)3-4-14-15-5-6-17(24-25(21,22)23)19(15,2)10-8-16(14)18;1-2-4-6-5-3-1/h11,14-17H,3-10H2,1-2H3,(H,21,22,23);1-5H/t14-,15-,16-,17-,18-,19-;/m0./s1
|
| Chemical Name |
[(8R,9S,10R,13S,14S,17S)-10,13-dimethyl-3-oxo-1,2,6,7,8,9,11,12,14,15,16,17-dodecahydrocyclopenta[a]phenanthren-17-yl] hydrogen sulfate;pyridine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (223.42 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.59 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.59 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.59 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2342 mL | 11.1709 mL | 22.3419 mL | |
| 5 mM | 0.4468 mL | 2.2342 mL | 4.4684 mL | |
| 10 mM | 0.2234 mL | 1.1171 mL | 2.2342 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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