| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 1g |
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| Other Sizes |
Purity: =99.4%
| Targets |
Human Endogenous Metabolite
19-Hydroxyandrost-4-ene-3,17-dione targets the enzyme aromatase (CYP19A1). It is a natural substrate and intermediate in the aromatization process, where the C19 methyl group is hydroxylated as the first step in the conversion of androgens to estrogens. As an endogenous metabolite, it does not have a specific receptor target but is a key molecule in steroidogenesis. |
|---|---|
| ln Vitro |
- 19-Hydroxyandrost-4-ene-3,17-dione is an androgen derivative that participates in estrogen biosynthesis, as indicated by the study’s focus on its kinetic role in this metabolic pathway. It is classified under the MeSH terms "Androgens" and "Androstenedione" derivatives, suggesting it acts as an intermediate or regulatory molecule in the conversion of androgens to estrogens [1]
- The study (published in Steroids 1965) primarily conducts kinetic analyses to investigate how 19-Hydroxyandrost-4-ene-3,17-dione contributes to estrogen biosynthesis, though specific details of its mechanism (e.g., interaction with key enzymes like aromatase) are not available in the provided literature metadata [1] In vitro, 19-Hydroxyandrost-4-ene-3,17-dione is used as a substrate to study the activity of the aromatase enzyme. Its conversion to estrone is measured in assays using microsomal preparations or recombinant CYP19A1. It is also used as a reference standard in analytical chemistry for the identification and quantification of steroid metabolites. It is not typically used for cell-based functional assays. |
| ln Vivo |
In vivo, 19-Hydroxyandrost-4-ene-3,17-dione is an endogenous intermediate in estrogen biosynthesis. It is produced in the adrenal glands and gonads. Its levels can be altered in certain pathological conditions, such as polycystic ovary syndrome (PCOS) and some forms of congenital adrenal hyperplasia. It is not used as a therapeutic agent.
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| Enzyme Assay |
For non-cell-based assays, 19-Hydroxyandrost-4-ene-3,17-dione can be used as a substrate in enzyme activity assays for aromatase. The compound is incubated with the enzyme (e.g., human placental microsomes or recombinant CYP19A1) and cofactors, and the production of estrone is measured by HPLC or mass spectrometry. It is also used as an analytical standard for the quantification of steroids in biological matrices.
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| Cell Assay |
For in vitro cellular assays, 19-Hydroxyandrost-4-ene-3,17-dione is not typically used for cell-based functional assays. It may be used in studies of steroidogenesis, where cells (e.g., adrenocortical cells or placental cells) are treated with the compound and its conversion to estrogens is measured. However, its primary use is as an analytical standard and a substrate for enzyme studies.
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| Animal Protocol |
For in vivo animal studies, 19-Hydroxyandrost-4-ene-3,17-dione is not typically administered for therapeutic or pharmacological studies. It may be used in metabolic studies to trace steroidogenesis pathways. However, its primary use is as a reference standard and a tool for studying aromatase activity in vitro.
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| ADME/Pharmacokinetics |
19-Hydroxyandrost-4-ene-3,17-dione has a molecular weight of 302.41 and a molecular formula of C19H26O3. It is a steroid. The compound is typically stored at -20°C for long-term stability. It is supplied with a purity of ≥97%. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of 19-Hydroxyandrost-4-ene-3,17-dione has not been extensively reported. As an endogenous steroid, its toxicity is not a primary concern at physiological levels. The compound is for research use only and is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References | |
| Additional Infomation |
19-Hydroxyandrost-4-ene-3,17-dione is a 19-hydroxy steroid, 3-oxo steroid, 17-oxo steroid, and androstane compound. It functions as a metabolite in mice.
19-Hydroxyandrost-4-ene-3,17-dione (CAS 510-64-5) is a steroid metabolite and a 19-hydroxy steroid. It is an endogenous intermediate in the biosynthesis of estrogens, specifically in the conversion of androstenedione to estrone by aromatase. It plays a role as a mouse metabolite. It is available for research purposes only. |
| Molecular Formula |
C19H26O3
|
|---|---|
| Molecular Weight |
302.41
|
| Exact Mass |
302.188
|
| CAS # |
510-64-5
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| PubChem CID |
252379
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
485.4±45.0 °C at 760 mmHg
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| Melting Point |
168-169ºC(lit.)
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| Flash Point |
261.5±25.2 °C
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| Vapour Pressure |
0.0±2.8 mmHg at 25°C
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| Index of Refraction |
1.571
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| LogP |
1.29
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
|
| Heavy Atom Count |
22
|
| Complexity |
563
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| Defined Atom Stereocenter Count |
5
|
| SMILES |
C[C@]12CC[C@H]3[C@H]([C@@H]1CCC2=O)CCC4=CC(=O)CC[C@]34CO
|
| InChi Key |
XGUHPTGEXRHMQQ-BGJMDTOESA-N
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| InChi Code |
InChI=1S/C19H26O3/c1-18-8-7-16-14(15(18)4-5-17(18)22)3-2-12-10-13(21)6-9-19(12,16)11-20/h10,14-16,20H,2-9,11H2,1H3/t14-,15-,16-,18-,19+/m0/s1
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| Chemical Name |
(8R,9S,10S,13S,14S)-10-(hydroxymethyl)-13-methyl-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthrene-3,17-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (330.68 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.27 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.27 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.27 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3068 mL | 16.5338 mL | 33.0677 mL | |
| 5 mM | 0.6614 mL | 3.3068 mL | 6.6135 mL | |
| 10 mM | 0.3307 mL | 1.6534 mL | 3.3068 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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