| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| 500mg |
|
||
| Other Sizes |
| Targets |
Benzophenone-d10 does not have a specific pharmacological receptor target. As a stable isotope-labeled compound, it is used as a tracer in analytical studies. Benzophenone itself is an endogenous metabolite and an impurity of Phenytoin. The deuterium labeling allows for the tracking of the compound in biological samples using mass spectrometry.
|
|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, Benzophenone-d10 is not used for biological activity assays. Its primary utility is as an internal standard and tracer in analytical chemistry. Stable heavy isotopes of hydrogen and other elements have been incorporated into drug molecules as tracers for quantitation during the drug development process. Benzophenone is an endogenously produced metabolite. |
| ln Vivo |
In vivo, Benzophenone-d10 is not administered for therapeutic purposes. Its primary use is as an internal standard in analytical studies to quantify benzophenone levels in biological samples. By using the deuterated analog, researchers can accurately measure the concentration of benzophenone in pharmacokinetic and metabolic studies. Deuteration may affect the pharmacokinetic and metabolic properties of a compound.
|
| Enzyme Assay |
For non-cell-based assays, Benzophenone-d10 is used as an analytical standard. Its identity and purity are confirmed using techniques such as NMR, HPLC, and mass spectrometry. The compound is typically supplied with a purity of 95% by HPLC and 98% atom% D. It is not used in receptor binding assays. Its role is to serve as a quantitative tracer in analytical method development.
|
| Cell Assay |
For in vitro cellular assays, Benzophenone-d10 is not typically used. It may be used in cell culture media as a part of sample preparation for LC-MS/MS analysis to quantify benzophenone uptake or metabolism. The compound is added to biological samples at a known concentration to serve as an internal standard, correcting for variability during sample processing and analysis.
|
| Animal Protocol |
For in vivo animal studies, Benzophenone-d10 is used as an internal standard for the quantification of benzophenone in pharmacokinetic and metabolic studies. Blood or tissue samples collected from animals are spiked with a known amount of Benzophenone-d10. The samples are then processed and analyzed by LC-MS/MS. The ratio of the signal of the analyte to that of the internal standard is used to calculate the concentration of the analyte.
|
| ADME/Pharmacokinetics |
Benzophenone-d10 has a molecular weight of 192.28 g/mol and a molecular formula of C13D10O. It has a melting point of 47-51°C and a boiling point of 305°C. The compound is soluble in chloroform and methanol. It should be stored at 2-8°C. It is for research use only and is not intended for human consumption.
|
| Toxicity/Toxicokinetics |
The toxicity profile of Benzophenone-d10 is not applicable, as it is used as an analytical standard at very low concentrations. The deuterium substitution is not expected to introduce new toxicities, as deuterium is a stable, non-radioactive isotope of hydrogen. The compound is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
|
| References | |
| Additional Infomation |
Benzophenone-d10 (CAS 22583-75-1) is the deuterium-labeled form of Benzophenone. Benzophenone is an impurity of Phenytoin, an anticonvulsant, and is an endogenously produced metabolite. Benzophenone-d10 is used as an internal standard for the quantification of benzophenone by GC- or LC-MS. It is also used in the manufacturing of antihistamines. It is available for research purposes only.
|
| Molecular Formula |
C13D10O
|
|---|---|
| Molecular Weight |
192.28
|
| Exact Mass |
192.135
|
| CAS # |
22583-75-1
|
| Related CAS # |
Benzophenone;119-61-9
|
| PubChem CID |
3035154
|
| Appearance |
White to off-white solid powder
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
305.4±0.0 °C at 760 mmHg
|
| Melting Point |
47-51ºC(lit.)
|
| Flash Point |
123.7±13.7 °C
|
| Vapour Pressure |
0.0±0.6 mmHg at 25°C
|
| Index of Refraction |
1.584
|
| LogP |
3.18
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
1
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
14
|
| Complexity |
165
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
[2H]C1=C([2H])C([2H])=C(C(C2=C([2H])C([2H])=C([2H])C([2H])=C2[2H])=O)C([2H])=C1[2H]
|
| InChi Key |
RWCCWEUUXYIKHB-LHNTUAQVSA-N
|
| InChi Code |
InChI=1S/C13H10O/c14-13(11-7-3-1-4-8-11)12-9-5-2-6-10-12/h1-10H/i1D,2D,3D,4D,5D,6D,7D,8D,9D,10D
|
| Chemical Name |
bis(2,3,4,5,6-pentadeuteriophenyl)methanone
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ≥ 100 mg/mL (~520.1 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.00 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (13.00 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2007 mL | 26.0037 mL | 52.0075 mL | |
| 5 mM | 1.0401 mL | 5.2007 mL | 10.4015 mL | |
| 10 mM | 0.5201 mL | 2.6004 mL | 5.2007 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
