| Size | Price | |
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| Other Sizes |
| Targets |
Methyl β-D-Galactopyranoside targets enzymes that recognize galactose-containing substrates, such as β-galactosidase. It acts as a substrate for β-galactosidase, which hydrolyzes the glycosidic bond to release galactose and methanol. It does not target a specific receptor but is used as a tool to study glycosidase activity.
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|---|---|
| ln Vitro |
In vitro, Methyl β-D-Galactopyranoside is used as a substrate in enzymatic assays to measure the activity of β-galactosidase and other glycosidases. The hydrolysis of the compound releases galactose, which can be measured using colorimetric or fluorometric methods. It is also used as a standard in carbohydrate chemistry and as a model compound for studying glycosidic bond cleavage.
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| ln Vivo |
In vivo, Methyl β-D-Galactopyranoside is not used for therapeutic purposes. Its primary applications are in biochemical research and as a reference standard. The compound is not intended for human consumption and is for research use only. Comprehensive in vivo pharmacological studies are not available.
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| Enzyme Assay |
For non-cell-based assays, Methyl β-D-Galactopyranoside is used as a substrate in enzyme activity assays. The compound is incubated with the enzyme (e.g., β-galactosidase), and the release of galactose is measured using colorimetric or fluorometric methods. It is also used as a standard in carbohydrate analysis and as a model compound for studying glycosidic bond cleavage.
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| Cell Assay |
For in vitro cellular assays, Methyl β-D-Galactopyranoside is not typically used for cell-based functional assays. It may be used in studies of carbohydrate metabolism, where cells are treated with the compound and its metabolism is assessed. However, its primary use is as a biochemical reagent rather than a pharmacological tool.
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| Animal Protocol |
For in vivo animal studies, Methyl β-D-Galactopyranoside is not typically used for therapeutic or pharmacological studies. It may be used in metabolic studies where it is administered to animals and its metabolism and distribution are tracked using analytical methods. However, its primary use is as a research reagent in biochemistry.
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| ADME/Pharmacokinetics |
Methyl β-D-Galactopyranoside has a molecular weight of 194.18 and a molecular formula of C7H14O6. It is a white crystalline powder. The compound is soluble in water and methanol. It should be stored at room temperature. It is available with high purity for research use. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of Methyl β-D-Galactopyranoside has not been extensively reported. As a carbohydrate derivative, it is expected to have low toxicity. The compound is for research use only and is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| Additional Infomation |
Methyl β-D-galactoside is a β-D-galactopyranoside with a methyl substituent at its terminal carbon atom. It is a β-D-galactoside, a monosaccharide derivative, and also a methyl D-galactoside. Methyl β-D-galactoside is a metabolite found or produced in Escherichia coli (strains K12 and MG1655). It has been reported that methyl β-D-galactopyranoside exists in Pogostemon cablin and Ahnfeltia plicata, and relevant data are available for reference.
Methyl β-D-Galactopyranoside (CAS 1824-94-8) is a methyl glycoside of β-D-galactose. It is used as a substrate in enzymatic assays to measure the activity of β-galactosidase and other glycosidases. It is also used as a standard in carbohydrate chemistry. It is available for research purposes only. |
| Molecular Formula |
C7H14O6
|
|---|---|
| Molecular Weight |
194.18
|
| Exact Mass |
194.079
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| CAS # |
1824-94-8
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| PubChem CID |
94214
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
389.1±42.0 °C at 760 mmHg
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| Melting Point |
172-180ºC
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| Flash Point |
189.1±27.9 °C
|
| Vapour Pressure |
0.0±2.0 mmHg at 25°C
|
| Index of Refraction |
1.548
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| LogP |
-2.69
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
13
|
| Complexity |
163
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
CO[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O
|
| InChi Key |
HOVAGTYPODGVJG-VOQCIKJUSA-N
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| InChi Code |
InChI=1S/C7H14O6/c1-12-7-6(11)5(10)4(9)3(2-8)13-7/h3-11H,2H2,1H3/t3-,4+,5+,6-,7-/m1/s1
|
| Chemical Name |
(2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-methoxyoxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (1287.47 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (514.99 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.1499 mL | 25.7493 mL | 51.4986 mL | |
| 5 mM | 1.0300 mL | 5.1499 mL | 10.2997 mL | |
| 10 mM | 0.5150 mL | 2.5749 mL | 5.1499 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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