![5,6-Dimethyl-1H-benzo[d]imidazole](/upload/1124/V70868.png)
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| Targets |
Human Endogenous Metabolite
5,6-Dimethyl-1H-benzo[d]imidazole does not have a specific pharmacological receptor target. As an endogenous metabolite, it is an intermediate in Riboflavin metabolism. Dimethylbenzimidazole is the second to last step for the synthesis of alpha-Ribazole. Its role is in metabolic pathways rather than as a signaling molecule targeting a specific receptor. |
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| ln Vitro |
In vitro, 5,6-Dimethyl-1H-benzo[d]imidazole is an endogenous metabolite. It is an intermediate in Riboflavin metabolism. It is not typically used for cell-based functional assays. Its primary applications are as a reference standard in analytical chemistry and as a tool for studying riboflavin metabolism.
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| ln Vivo |
In vivo, 5,6-Dimethyl-1H-benzo[d]imidazole is an endogenously produced metabolite. It has a role as an Escherichia coli metabolite and a human metabolite. It is an intermediate in Riboflavin metabolism. It is not used as a therapeutic agent. Its primary relevance is in the study of metabolism and as a biomarker.
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| Enzyme Assay |
For non-cell-based assays, 5,6-Dimethyl-1H-benzo[d]imidazole is used as a reference standard and a chemical intermediate. Its identity and purity are confirmed using techniques such as NMR, HPLC, and mass spectrometry. It is not used in receptor binding assays. Its role is to serve as a building block in organic synthesis and as a standard in analytical method development.
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| Cell Assay |
For in vitro cellular assays, 5,6-Dimethyl-1H-benzo[d]imidazole is not typically used for cell-based functional assays. It may be used in studies of riboflavin metabolism, where cells are treated with the compound and its metabolism is assessed. However, its primary use is as a chemical reagent rather than a pharmacological tool.
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| Animal Protocol |
For in vivo animal studies, 5,6-Dimethyl-1H-benzo[d]imidazole is not typically used for therapeutic or pharmacological studies. It may be used in metabolic studies where it is administered to animals and its metabolism and distribution are tracked using analytical methods. However, its primary use is as a chemical intermediate rather than a research tool for in vivo studies.
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| ADME/Pharmacokinetics |
5,6-Dimethyl-1H-benzo[d]imidazole has a molecular weight of 146.19 and a molecular formula of C9H10N2. It has a purity of ≥95%. The compound is an endogenously produced metabolite. It should be stored according to the manufacturer's recommendations. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of 5,6-Dimethyl-1H-benzo[d]imidazole has not been extensively reported. As an endogenous metabolite, it is not expected to be toxic at physiological concentrations. The compound is for research use only and is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| Additional Infomation |
5,6-Dimethylbenzimidazole is a dimethylbenzimidazole with methyl substituents at the 5 and 6 positions. It is a metabolite of both E. coli and humans. Dimethylbenzimidazole is a metabolite found in or produced by E. coli strains (K12 and MG1655).
5,6-Dimethyl-1H-benzo[d]imidazole (5,6-Dimethylbenzimidazole, CAS 582-60-5) is an endogenously produced metabolite. It is a dimethylbenzimidazole carrying methyl substituents at positions 5 and 6. It has a role as an Escherichia coli metabolite and a human metabolite. It is an intermediate in Riboflavin metabolism. It is available for research purposes only. |
| Molecular Formula |
C9H10N2
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|---|---|
| Molecular Weight |
146.19
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| Exact Mass |
146.084
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| CAS # |
582-60-5
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| PubChem CID |
675
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| Appearance |
White to light yellow solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
376.8±11.0 °C at 760 mmHg
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| Melting Point |
205.5 °C
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| Flash Point |
208.6±5.7 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.645
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| LogP |
2.31
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
11
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| Complexity |
147
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=CC2=C(C=C1C)N=CN2
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| InChi Key |
LJUQGASMPRMWIW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H10N2/c1-6-3-8-9(4-7(6)2)11-5-10-8/h3-5H,1-2H3,(H,10,11)
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| Chemical Name |
5,6-dimethyl-1H-benzimidazole
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (684.04 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (17.10 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (17.10 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (17.10 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.8404 mL | 34.2021 mL | 68.4041 mL | |
| 5 mM | 1.3681 mL | 6.8404 mL | 13.6808 mL | |
| 10 mM | 0.6840 mL | 3.4202 mL | 6.8404 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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