Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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Targets |
EGFRL858R/T790M/C797S EGFRdel19 T790M C797S
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ln Vitro |
EMI56 can be employed in the study of drug-resistant non-small-cell lung cancer (NSCLC) linked with mutant EGFR since it inhibits both EGFR ex19del/T790M/C797S and EGFR L858R/T790M/C797S[1]. PC9 EGFR ex19del/T790M/C797S cells exhibit substantial inhibition of total EGFR levels, activation, and downstream signaling under treatment with EMI56 (10, 15, 20 μM) for two hours[1].
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Cell Assay |
Cell Viability Assay[1]
Cell Types: PC9 EGFR ex19del/T790M/C797S cells Tested Concentrations: 1, 5, 10, 15, 20 μM Incubation Duration: 2 hrs (hours) Experimental Results: Inhibited total EGFR levels from 10 μM concentration. |
References |
[1]. Punit Saraon, et al. A Drug Discovery Platform to Identify Compounds That Inhibit EGFR Triple Mutants.Nat Chem Biol. 2020 May;16(5):577-586.
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Molecular Formula |
C21H20N2O3
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Molecular Weight |
348.40
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CAS # |
2414374-41-5
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SMILES |
O1C(C(C2=NC3C=CC=CC=3O2)=CC2C=C(C)C(=CC1=2)N(CC)CC)=O
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 50 mg/mL (143.51 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (5.97 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.8703 mL | 14.3513 mL | 28.7026 mL | |
5 mM | 0.5741 mL | 2.8703 mL | 5.7405 mL | |
10 mM | 0.2870 mL | 1.4351 mL | 2.8703 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.