| Size | Price | Stock | Qty |
|---|---|---|---|
| 50g |
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| Other Sizes |
| Targets |
No specific biological target is associated with ammonium bicarbonate, as it functions primarily as a chemical reagent and buffer rather than a direct pharmacological agent. However, the compound provides ammonium and bicarbonate ions that are involved in various physiological processes. Ammonium ions are important for nitrogen metabolism and acid-base balance, while bicarbonate ions are essential for pH regulation in biological systems. The compound's role as a buffer allows it to maintain pH in biochemical assays and cell culture media. In the pharmaceutical industry, it is used as an excipient in drug formulations, where it may affect drug stability and bioavailability. The compound's decomposition to ammonia and carbon dioxide can influence local pH and gas concentrations in biological systems.
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|---|---|
| ln Vitro |
In vitro, ammonium bicarbonate is used as a buffer in biochemical research and as a reagent in chemical synthesis. In the food industry, it is used as a leavening agent. In the pharmaceutical industry, it is used as an excipient in drug formulations. The compound is also used in various industrial applications including as a fertilizer and in the production of other ammonium salts. In biochemical assays, it is used to provide ammonium ions for studying nitrogen metabolism and to maintain pH in enzyme reactions. The compound's ability to decompose to ammonia and carbon dioxide makes it useful for generating gas in certain applications. In molecular biology, it may be used in protein precipitation and purification procedures.
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| ln Vivo |
In vivo, ammonium bicarbonate is not typically administered as a therapeutic agent. However, it is used in the food industry as a leavening agent and may be consumed as a food additive. When ingested, the compound decomposes to ammonia and carbon dioxide, which are metabolized and excreted. The compound's use as a pharmaceutical excipient means that it may be present in drug formulations administered to patients. The compound's effects in vivo are primarily related to its role as a source of ammonium and bicarbonate ions, which are involved in normal physiological processes. High doses may cause toxicity due to ammonia accumulation.
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| Enzyme Assay |
Cell-free assays for ammonium bicarbonate involve its use as a buffer and reagent. Standard protocols for buffer preparation involve dissolving the compound in water and adjusting the pH to the desired value. The buffer is used in various biochemical assays including enzyme kinetics, protein purification, and cell culture applications. The compound's ability to maintain pH can be assessed by measuring the pH of the buffer solution under various conditions. Its use as a reagent in chemical synthesis involves standard procedures for the preparation of other ammonium salts and for reactions requiring a source of ammonia. The compound's decomposition to ammonia and carbon dioxide can be studied using gas chromatography or other analytical techniques.
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| Cell Assay |
Cellular assays using ammonium bicarbonate involve its use as a buffer in cell culture media. The compound is used to maintain physiological pH in cell culture, and cell viability and function are evaluated in the presence of the buffer. The compound's compatibility with cell growth and function is assessed. In studies of nitrogen metabolism, the compound may be used to provide ammonium ions to cells. The compound's effects on cellular pH and metabolism can be studied using various assays. However, the compound itself is not typically used as a test article in cellular assays beyond its role as a buffer component.
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| Animal Protocol |
Animal studies for ammonium bicarbonate are not typically conducted, as the compound is primarily a chemical reagent and food additive rather than a pharmacological agent. However, the compound's toxicity has been studied in animals. The compound is classified with the primary hazard being the threat to the environment. When administered in high doses, the compound can cause toxicity due to ammonia accumulation. The compound decomposes above 35 °C, producing ammonia fumes that can be toxic upon inhalation. The compound reacts violently with acids and reacts with strong bases and strong oxidants.
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| ADME/Pharmacokinetics |
Absorption, Distribution and Excretion
Compared to ammonium chloride infusion, ammonia excretion appears to be faster after ammonium bicarbonate infusion, resulting in higher levels of unionized ammonia in the blood. Metabolism/Metabolites Ammonium bicarbonate dissociates into free ammonia at the cell membrane level. Pharmacokinetic data for ammonium bicarbonate are not applicable, as the compound is primarily a chemical reagent and food additive rather than a drug. When ingested, the compound decomposes to ammonia and carbon dioxide, which are metabolized and excreted. Ammonia is converted to urea in the liver and excreted by the kidneys. Carbon dioxide is exhaled or converted to bicarbonate and excreted. The compound's rapid decomposition means that it does not persist in the body. The compound's use as a pharmaceutical excipient may influence the pharmacokinetics of the drug by affecting pH and solubility. |
| Toxicity/Toxicokinetics |
Non-Human Toxicity Values
Mouse intravenous LD50: 245 mg/kg Toxicological data for ammonium bicarbonate indicate that the primary hazard is the threat to the environment. The compound decomposes above 35 °C, producing ammonia fumes. It reacts violently with acids and reacts with strong bases and strong oxidants. The compound appears as a white crystalline solid having the odor of ammonia and is soluble in water. Appropriate safety precautions should be taken when handling the compound, including the use of personal protective equipment such as gloves and safety goggles. The compound should be handled in a well-ventilated area, and contact with skin and eyes should be avoided. In case of exposure, affected areas should be rinsed thoroughly with water. |
| Additional Infomation |
Ammonium bicarbonate is a white crystalline solid with an ammonia odor and is readily soluble in water. Its main hazard lies in its environmental threat. Immediate measures should be taken to limit its spread into the environment. Ammonium bicarbonate is used in the manufacture of other ammonium compounds, food processing, and other applications.
Ammonium bicarbonate is an oxygen-containing organic compound. Mechanism of Action The principle behind ammonium bicarbonate's antifungal action is that the bicarbonate anion provides the necessary alkalinity, thereby establishing the free ammonia concentration required for antifungal activity. Therapeutic Uses Antacid and stimulant. Dosage: 0.3-1 gram. /Ammonium Bicarbonate BP/ Veterinary Drugs: Expectorant, used to treat flatulence and colic. Expectorant Ammonium bicarbonate is a research chemical and industrial reagent, not an approved drug. It has no clinical trial or marketing approval status for therapeutic use. The compound is an inorganic salt widely used in the food industry as a leavening agent, in the pharmaceutical industry as an excipient, and in various industrial applications. It is also used as a buffer in biochemical research and as a reagent in chemical synthesis. The compound has a molecular formula of NH4HCO3 and a molecular weight of 79.05 g/mol. It appears as a white crystalline solid having the odor of ammonia and is soluble in water. The compound decomposes above 35 °C, producing ammonia fumes. It should be stored in a cool, dry place away from acids and oxidizing agents. |
| Molecular Formula |
CH5NO3
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|---|---|
| Molecular Weight |
79.06
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| Exact Mass |
79.026
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| CAS # |
1066-33-7
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| Related CAS # |
10361-29-2;10361-29-2 (unspecified ammonium salt);40861-29-8 (ammonium-zinc salt)
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| PubChem CID |
14013
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| Appearance |
Colorless or white crystals
Shiny, hard, colorless or white prisms or crystalline mass |
| Density |
1,586 g/cm3
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| Boiling Point |
169.8ºC
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| Melting Point |
95 °F (USCG, 1999)
; 107 °C (decomposes)
; Decomposes below its melting point, dissociating into ammonia, carbon dioxide and water.
; 36 °C
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| Vapour Pressure |
2.58E-05mmHg at 25°C
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| LogP |
0.546
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
5
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| Complexity |
24.8
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O([H])C(=O)[O-].[N+]([H])([H])([H])[H]
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| InChi Key |
ATRRKUHOCOJYRX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/CH2O3.H3N/c2-1(3)4;/h(H2,2,3,4);1H3
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| Chemical Name |
azanium;hydrogen carbonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50 mg/mL (632.43 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (31.62 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (31.62 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (31.62 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 12.6486 mL | 63.2431 mL | 126.4862 mL | |
| 5 mM | 2.5297 mL | 12.6486 mL | 25.2972 mL | |
| 10 mM | 1.2649 mL | 6.3243 mL | 12.6486 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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