| Size | Price | Stock | Qty |
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| 25g |
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| 50g |
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| Other Sizes |
| Targets |
5-Amino-2-methoxypyridine is used as a reagent in the synthesis of a novel series of thienopyrimidines as highly potent and selective PI3K inhibitors. The compound does not have a defined pharmacological target of its own but serves as a building block for PI3K inhibitors. Its structure features a pyridine ring with amino and methoxy groups, making it an important intermediate in the synthesis of various drugs and agrochemicals.
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| ln Vitro |
In vitro, 5-Amino-2-methoxypyridine is primarily used as a chemical reagent and synthetic intermediate. It is used as a reagent in the synthesis of a novel series of thienopyrimidines as highly potent and selective PI3K inhibitors. The compound is employed in the synthesis of various pharmaceutical compounds that are subsequently tested in in vitro assays. It is soluble in chloroform and methanol.
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| ln Vivo |
In vivo activity data for 5-Amino-2-methoxypyridine itself are not available, as the compound is not intended for direct in vivo administration. Rather, it is a precursor used in the synthesis of drug candidates, such as PI3K inhibitors, that are subsequently evaluated in animal models. The compound's in vivo relevance is indirect, through the biological activities of the final pharmaceutical compounds derived from it.
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| Enzyme Assay |
For in vitro enzyme/receptor binding studies, 5-Amino-2-methoxypyridine is not typically evaluated as a test compound itself. Instead, it serves as a reagent for the synthesis of test compounds. The compound's properties include a molecular weight of 124.14 g/mol. The amino and methoxy groups provide handles for further derivatization in medicinal chemistry for the synthesis of PI3K inhibitors and other pharmaceutical compounds.
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| Cell Assay |
For in vitro cell-based experiments, 5-Amino-2-methoxypyridine is used as an intermediate in the synthesis of compounds that are tested in cell viability, proliferation, and cytotoxicity assays. The compound itself is not typically evaluated in cell-based assays. Standard cell culture protocols for test compounds synthesized from this intermediate involve dissolving the final product in DMSO and diluting to working concentrations in appropriate cell culture medium.
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| Animal Protocol |
In vivo animal studies using 5-Amino-2-methoxypyridine are conducted on the final drug compounds synthesized from it, not on the intermediate itself. For PI3K inhibitors derived from this building block, efficacy studies would typically be performed in mouse models of cancer or other PI3K-related diseases. Standard in vivo protocols involve administration to rodents via oral gavage or intraperitoneal injection.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 5-Amino-2-methoxypyridine as a standalone compound are not characterized in the literature. The compound has a molecular weight of 124.14 g/mol, which is within the favorable range for oral bioavailability. The presence of the amino and methoxy groups may affect metabolic stability and permeability. Empirical pharmacokinetic data are not available for this intermediate compound.
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| Toxicity/Toxicokinetics |
5-Amino-2-methoxypyridine is a research chemical and should be handled with appropriate laboratory safety precautions. As an aminopyridine derivative, it may pose risks of skin and eye irritation. The compound should be stored at 2-8°C. Specific LD₅₀ values, acute toxicity classifications, and chronic toxicity data are not available in the public literature. Standard safety practices include the use of personal protective equipment and working in a fume hood.
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| Additional Infomation |
6-Methoxypyridine-3-amine is an aminopyridine.
5-Amino-2-methoxypyridine (CAS 6628-77-9) is primarily a research-grade chemical intermediate, not an FDA-approved pharmaceutical drug. Its primary applications are in organic synthesis as a building block for pharmaceutical compounds, including highly potent and selective PI3K inhibitors. The compound serves as an important intermediate in the synthesis of various drugs and agrochemicals. No clinical trials or approved indications exist for this compound. It is intended for laboratory research use only. |
| Molecular Formula |
C6H8N2O
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|---|---|
| Molecular Weight |
124.14
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| Exact Mass |
124.063
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| CAS # |
6628-77-9
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| PubChem CID |
81121
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| Appearance |
Brown to reddish brown <29°C solid powder,>31°C liquid
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
264.8±0.0 °C at 760 mmHg
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| Melting Point |
29-31 °C(lit.)
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| Flash Point |
109.5±21.8 °C
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| Vapour Pressure |
0.0±0.5 mmHg at 25°C
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| Index of Refraction |
1.561
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| LogP |
0.47
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
9
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| Complexity |
87.1
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC1=NC=C(C=C1)N
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| InChi Key |
UUVDJIWRSIJEBS-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H8N2O/c1-9-6-3-2-5(7)4-8-6/h2-4H,7H2,1H3
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| Chemical Name |
6-methoxypyridin-3-amine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~805.5 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (20.14 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (20.14 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 8.0554 mL | 40.2771 mL | 80.5542 mL | |
| 5 mM | 1.6111 mL | 8.0554 mL | 16.1108 mL | |
| 10 mM | 0.8055 mL | 4.0277 mL | 8.0554 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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