| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
|
||
| Other Sizes |
| Targets |
4-Nitrophenyl butyrate is a substrate for lipase enzymes. Upon hydrolysis by lipases, 4-NPB is converted into p-nitrophenoxide, which can be monitored using UV-Vis spectrophotometry at 400 nm to quantify lipase activity. The compound does not have a pharmacological target but serves as a research tool for measuring enzyme activity.
|
|---|---|
| ln Vitro |
4-Nitrophenyl butyrate is a biochemical reagent that can be utilized in life science research as an organic compound or biological material.
In vitro, 4-nitrophenyl butyrate is used as a colorimetric substrate for lipases. Upon hydrolysis, 4-NPB is converted into p-nitrophenoxide, which can be monitored using UV-Vis spectrophotometry at 400 nm to quantify lipase activity. The compound is widely used in enzyme activity assays, high-throughput screening, and biochemical research to study lipase function and inhibition. |
| ln Vivo |
In vivo data for 4-nitrophenyl butyrate as a therapeutic agent are not available, as the compound is a chromogenic substrate used exclusively for in vitro enzymatic assays. It is not intended for in vivo administration and has no established in vivo pharmacokinetic or pharmacodynamic profile. The compound is not designed for therapeutic use.
|
| Enzyme Assay |
For in vitro enzyme activity assays, 4-nitrophenyl butyrate is used as a substrate for lipases. Standard protocols involve dissolving the compound in an appropriate solvent (e.g., DMSO, isopropanol) and adding it to the enzyme solution in buffer. The release of p-nitrophenol is monitored at 400-405 nm over time using a spectrophotometer or plate reader. The initial rate of absorbance increase is proportional to lipase activity.
|
| Cell Assay |
For in vitro cell-based experiments, 4-nitrophenyl butyrate is not typically used directly in cell culture. It is a chromogenic substrate used for measuring lipase activity in cell lysates or biological fluids. Cells are lysed in appropriate buffer, and the substrate is added to the lysate. After incubation, absorbance is measured at 400 nm to quantify lipase activity.
|
| Animal Protocol |
In vivo animal studies for 4-nitrophenyl butyrate are not applicable, as the compound is a chromogenic substrate used exclusively for in vitro enzymatic assays. No animal studies have been conducted for this compound as a therapeutic agent, and it is not intended for diagnostic or therapeutic use in animals or humans.
|
| ADME/Pharmacokinetics |
Pharmacokinetic data for 4-nitrophenyl butyrate are not available, as the compound is not a drug and is not administered to living organisms. The compound has a molecular weight of 209.20 g/mol. The ester bond is susceptible to hydrolysis by esterases, which would release p-nitrophenol and butyric acid. The compound is primarily used as a reagent rather than as a drug candidate.
|
| Toxicity/Toxicokinetics |
4-Nitrophenyl butyrate is a research chemical and should be handled with appropriate laboratory safety precautions. As a nitrophenyl ester, it may cause skin and eye irritation. The compound should be stored under appropriate conditions. Specific LD₅₀ values, acute toxicity classifications, and chronic toxicity data are not available in the public literature. Standard safety practices include the use of personal protective equipment and working in a fume hood.
|
| Additional Infomation |
p-Nitrophenyl butyrate is a butyrate ester formed by the condensation of the hydroxyl group of 4-nitrophenol with the carboxyl group of butyric acid. It is a butyrate ester and also a C-nitro compound. Its function is related to that of 4-nitrophenol.
4-Nitrophenyl butyrate (4-NPB, CAS 2635-84-9) is primarily a research-grade chromogenic substrate, not an FDA-approved pharmaceutical drug. Its primary application is as a colorimetric substrate for lipases, where hydrolysis produces p-nitrophenoxide that can be monitored spectrophotometrically at 400 nm to quantify lipase activity. No clinical trials or approved therapeutic indications exist for this compound. |
| Molecular Formula |
C10H11NO4
|
|---|---|
| Molecular Weight |
209.20
|
| Exact Mass |
209.068
|
| CAS # |
2635-84-9
|
| PubChem CID |
75834
|
| Appearance |
Colorless to light yellow liquid（Density：1.19 g/cm3）
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
323.2±25.0 °C at 760 mmHg
|
| Flash Point |
144.8±25.2 °C
|
| Vapour Pressure |
0.0±0.7 mmHg at 25°C
|
| Index of Refraction |
1.534
|
| LogP |
2.55
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
15
|
| Complexity |
228
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O(C1C([H])=C([H])C(=C([H])C=1[H])[N+](=O)[O-])C(C([H])([H])C([H])([H])C([H])([H])[H])=O
|
| InChi Key |
DVDUMIQZEUTAGK-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3
|
| Chemical Name |
(4-nitrophenyl) butanoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 100 mg/mL (478.01 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.95 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7801 mL | 23.9006 mL | 47.8011 mL | |
| 5 mM | 0.9560 mL | 4.7801 mL | 9.5602 mL | |
| 10 mM | 0.4780 mL | 2.3901 mL | 4.7801 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
