| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
Dithizone does not have a defined pharmacological target. It is an organosulfur compound that acts as a chelating agent and forms complexes with metals such as lead and mercury. Its mechanism of action is chemical rather than biological: the compound's thiocarbazone group coordinates with metal ions to form stable, colored complexes that can be detected and quantified.
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|---|---|
| ln Vitro |
In vitro, dithizone is used as a chelating agent for zinc and other metals. It is widely used in analytical chemistry for the detection and quantification of various metals since it forms stable complexes with them. The compound is used in colorimetric assays for metal ion determination and in the extraction and preconcentration of trace metals from various samples.
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| ln Vivo |
In vivo data for dithizone as a therapeutic agent are not available, as the compound is a chemical reagent used for analytical applications. It is not intended for in vivo administration and has no established in vivo pharmacokinetic or pharmacodynamic profile. The compound is not designed for therapeutic use.
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| Enzyme Assay |
For in vitro analytical assays, dithizone is used as a reagent for the detection and quantification of metal ions. Standard protocols involve dissolving the compound in an appropriate solvent (e.g., chloroform, carbon tetrachloride) and adding it to aqueous samples containing metal ions. The formation of colored metal-dithizone complexes is measured spectrophotometrically. The compound is also used in solvent extraction methods for metal ion separation and preconcentration.
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| Cell Assay |
For in vitro cell-based experiments, dithizone is not typically used directly in cell culture. It is a chemical reagent used for analytical chemistry applications. The compound may be used in the preparation of metal complexes or in the analysis of cell culture samples, but it is not added directly to cell cultures as a test compound.
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| Animal Protocol |
In vivo animal studies for dithizone are not applicable, as the compound is a chemical reagent used for analytical applications. No animal studies have been conducted for this compound as a therapeutic agent, and it is not intended for diagnostic or therapeutic use in animals or humans.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for dithizone are not available, as the compound is not a drug and is not administered to living organisms. The compound has a molecular weight of 256.33 g/mol. As a sulfur-containing organic compound with low water solubility, it is expected to have limited oral bioavailability and poor membrane permeability.
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| Toxicity/Toxicokinetics |
Dithizone is a research chemical and should be handled with appropriate laboratory safety precautions. As a sulfur-containing organic compound, it may cause skin and eye irritation. The compound is for research use only and not for human therapeutic or diagnostic applications. Specific LD₅₀ values, acute toxicity classifications, and chronic toxicity data are not available in the public literature. Standard safety practices include the use of personal protective equipment and working in a fume hood.
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| Additional Infomation |
Dithizone belongs to the phenylhydrazine class of compounds. It is a chelating agent used to treat heavy metal poisoning and for detection. It can cause diabetes.
Dithizone (Diphenylthiocarbazone, CAS 60-10-6) is primarily a research-grade analytical reagent, not an FDA-approved pharmaceutical drug. Its primary applications are as a chelating agent for the detection and quantification of metals such as zinc, lead, and mercury in analytical chemistry. No clinical trials or approved therapeutic indications exist for this compound. |
| Molecular Formula |
C13H12N4S
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|---|---|
| Molecular Weight |
256.33
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| Exact Mass |
256.078
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| CAS # |
60-10-6
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| PubChem CID |
657262
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| Appearance |
Brown to black solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
376.1±25.0 °C at 760 mmHg
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| Melting Point |
168°C (dec.)
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| Flash Point |
181.2±23.2 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.644
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| LogP |
4.01
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
18
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| Complexity |
281
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=C(C=C1)NNC(=S)N=NC2=CC=CC=C2
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| InChi Key |
UOFGSWVZMUXXIY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H12N4S/c18-13(16-14-11-7-3-1-4-8-11)17-15-12-9-5-2-6-10-12/h1-10,14H,(H,16,18)
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| Chemical Name |
1-anilino-3-phenyliminothiourea
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50 mg/mL (195.06 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.75 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.75 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9012 mL | 19.5061 mL | 39.0122 mL | |
| 5 mM | 0.7802 mL | 3.9012 mL | 7.8024 mL | |
| 10 mM | 0.3901 mL | 1.9506 mL | 3.9012 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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