| Size | Price | Stock | Qty |
|---|---|---|---|
| 50g |
|
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| Other Sizes |
Purity: ≥98%
| Targets |
Zwitterionic detergent; It is a zwitterionic detergent that non-specifically interacts with lipid bilayers and hydrophobic protein domains to solubilize membrane proteins by disrupting lipid-protein and protein-protein interactions while maintaining protein stability in aqueous solutions.
DMAPS does not have a defined pharmacological target. It is a zwitterionic detergent used to solubilize membrane proteins in biochemical research. Its mechanism of action is physical: the detergent molecule inserts into lipid bilayers and disrupts membrane structure, allowing membrane proteins to be extracted into solution while maintaining their native conformation. |
|---|---|
| ln Vitro |
As a biochemical reagent, DMAPS is used for the solubilization and purification of membrane-bound proteins and other hydrophobic biomolecules due to its excellent detergency properties. The long hydrocarbon chain provides good membrane penetration and solubilization capabilities, while the sulfonate and quaternary ammonium groups ensure water solubility and charge neutrality.
3-(N,N-Dimethylmyristylammonio)propanesulfonate is a biochemical reagent that can be utilized in studies pertaining to life sciences as an organic substance or biological material. In vitro, DMAPS is used as a mild detergent for the solubilization and purification of membrane proteins. It is also used in protein crystallization and in studies of protein-lipid interactions. As a zwitterionic detergent, it is less denaturing than ionic detergents like SDS. |
| ln Vivo |
In vivo data for DMAPS as a therapeutic agent are not available, as the compound is a detergent used for in vitro applications. It is not intended for in vivo administration and has no established in vivo pharmacokinetic or pharmacodynamic profile.
|
| Enzyme Assay |
As a zwitterionic detergent, DMAPS is typically used as a solubilizing agent in protein purification workflows rather than as an active test compound.
For in vitro protein purification, DMAPS is used as a detergent to solubilize membrane proteins. Standard protocols involve dissolving the compound in an appropriate buffer at a concentration of 1-10 mM and adding it to the membrane preparation. The solubilized proteins are then separated from insoluble material by centrifugation and purified by chromatography. |
| Cell Assay |
DMAPS is generally used in cell-free systems for protein solubilization and purification.
For in vitro cell-based experiments, DMAPS is not typically used directly in cell culture. It is a detergent used for protein extraction and purification. The compound may be used in the preparation of membrane protein samples for subsequent analysis, but it is not added directly to cell cultures as a test compound. |
| Animal Protocol |
In vivo animal studies for DMAPS are not applicable, as the compound is a detergent used for in vitro applications. No animal studies have been conducted for this compound as a therapeutic agent.
|
| ADME/Pharmacokinetics |
Pharmacokinetic data for DMAPS are not available, as the compound is not a drug and is not administered to living organisms. The compound is a solid and should be stored under appropriate conditions.
|
| Toxicity/Toxicokinetics |
According to safety data, DMAPS is classified as an irritant (Signal Word: Warning). Hazard statements include H315 (Causes skin irritation), H319 (Causes serious eye irritation), and STOT SE 3 (May cause respiratory irritation). The product is not classified as a hazardous substance or mixture under GHS by some manufacturers, but other sources indicate it as an irritant. Recommended personal protective equipment includes safety goggles, protective gloves, impervious clothing, and suitable respirator. In case of eye contact, flush immediately with large amounts of water and call a physician. The product is for research use only and not for human or veterinary use. Carcinogenicity: Not listed as carcinogenic by NTP, IARC, OSHA, or ACGIH. California Prop. 65: Does not contain chemicals known to cause cancer, birth defects, or reproductive harm.
DMAPS is a research chemical and should be handled with appropriate laboratory safety precautions. As a detergent, it may cause skin and eye irritation. The compound is for research use only and not for human therapeutic or diagnostic applications. |
| References |
[1]. Interchain Spacing and Screening Length Modification of PSS Backbone Chains in Zwitterion-Doped Poly(3,4-Ethylenedioxythiophene):Polystyrene Sulfonate. Journal of Surface Investigation: X-ray, Synchrotron and Neutron Techniques; Publication Date: 2020-10-08; DOI: 10.1134/s102745102007037x
|
| Additional Infomation |
zwitterion 3-14 is an organic sulfonic acid.
DMAPS (Zwittergent 3-14) (CAS 14933-09-6) is primarily a research-grade biochemical reagent, not an FDA-approved pharmaceutical drug. Its primary application is as a mild, zwitterionic detergent for the solubilization and purification of membrane proteins. |
| Molecular Formula |
C19H41NO3S
|
|---|---|
| Molecular Weight |
363.60
|
| Exact Mass |
363.28
|
| CAS # |
14933-09-6
|
| PubChem CID |
84705
|
| Appearance |
White to off-white solid powder
|
| Melting Point |
250ºC
|
| Flash Point |
>110ºC
|
| LogP |
-1.05
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
16
|
| Heavy Atom Count |
24
|
| Complexity |
361
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CCCCCCCCCCCCCC[N+](C)(C)CCCS(=O)(=O)[O-]
|
| InChi Key |
BHATUINFZWUDIX-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C19H41NO3S/c1-4-5-6-7-8-9-10-11-12-13-14-15-17-20(2,3)18-16-19-24(21,22)23/h4-19H2,1-3H3
|
| Chemical Name |
3-[dimethyl(tetradecyl)azaniumyl]propane-1-sulfonate
|
| Synonyms |
3-(N,N-Dimethylmyristylammonio)propanesulfonate; Myristyl sulfobetaine; N-Tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate; Zwittergent 3-14; 3-[dimethyl(tetradecyl)azaniumyl]propane-1-sulfonate; MFCD00036910; 3-[dimethyl(tetradecyl)ammonio]propane-1-sulfonate;
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 8.33 mg/mL (22.91 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.83 mg/mL (2.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.83 mg/mL (2.28 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 0.83 mg/mL (2.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7503 mL | 13.7514 mL | 27.5028 mL | |
| 5 mM | 0.5501 mL | 2.7503 mL | 5.5006 mL | |
| 10 mM | 0.2750 mL | 1.3751 mL | 2.7503 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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