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| Other Sizes |
| Targets |
Ecamsule disodium functions as a sunscreen agent by absorbing ultraviolet (UV) radiation, specifically UVA rays. It reduces biological damage caused by solar radiation, such as the formation of pyrimidine dimers, the accumulation of p53 protein, or the expression of collagenase 2. It is known for its excellent photostability and water solubility.
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| ln Vitro |
The greatest absorption of EcamsuLe, a broad spectrum UVA absorber, is measured at 344 nm [1]. The sensitivity of wild-type Fibs E6/E7 cells to EcamsuLe treatment (200–1600 μM) is higher [1]. EcamsuLe inhibits ROS production that is produced by UV and AAPH [1]. This impact is dose-dependent; in a UV environment, the largest reduction in ROS occurs at 1600 μM, the highest tested concentration. Both the basal level and the oxidative stress brought on by AAPH decreased by 16.9% and 10.8%, respectively, at the same concentration [1]. When ecamsuLe is applied at the maximum dose of 1600 μM, it boosts viability in AAPH-treated cells [1].
In vitro, ecamsule disodium is evaluated for its UV absorption properties and photostability. The compound's ability to absorb UVA radiation is measured using UV-Vis spectrophotometry. It is also tested for its ability to protect skin cells from UV-induced damage in cell culture models, such as reducing the formation of pyrimidine dimers and the accumulation of p53 protein. |
| ln Vivo |
In vivo, ecamsule disodium is used topically in sunscreens to provide photoprotection against UVA radiation. When applied to the skin, it absorbs UVA rays and prevents them from penetrating the skin, thereby reducing the risk of sunburn, premature skin aging, and skin cancer. It is an active ingredient in sunscreens that protects the skin from sunburn and erythema.
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| Enzyme Assay |
For in vitro UV absorption assays, ecamsule disodium is evaluated for its ability to absorb UVA radiation. Standard protocols involve dissolving the compound in an appropriate solvent and measuring the UV-Vis absorption spectrum using a spectrophotometer. The compound's photostability is assessed by exposing it to UV radiation and monitoring changes in absorption over time.
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| Cell Assay |
For in vitro cell-based experiments, ecamsule disodium can be evaluated for its photoprotective effects on skin cells. Cells such as human keratinocytes are treated with the compound prior to UVA irradiation. UV-induced DNA damage (pyrimidine dimers) and protein expression (p53, collagenase 2) are measured to assess the compound's protective effects.
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| Animal Protocol |
In vivo animal studies for ecamsule disodium are typically conducted in animal models to evaluate its photoprotective efficacy and safety. Standard protocols involve applying the compound topically to the skin of rodents and exposing them to UVA radiation. The extent of UV-induced erythema, edema, and skin thickening is measured.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of ecamsule disodium are characterized in the context of topical application. When applied to the skin, a portion of the compound may be absorbed percutaneously. Systemic absorption is generally low, and the compound is not expected to accumulate in the body. Its water solubility influences its formulation and skin penetration characteristics.
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| Toxicity/Toxicokinetics |
The safety of ecamsule disodium has been evaluated by regulatory agencies. The compound is approved for use as a sunscreen ingredient. It may cause skin irritation or allergic reactions in some individuals. Standard safety practices should be followed when handling the compound in research settings.
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| References |
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| Additional Infomation |
Ecamsule disodium (CAS 90458-75-6) is a sunscreen agent, not an FDA-approved pharmaceutical drug. Its primary application is as an active ingredient in sunscreens to provide broad-spectrum UVA protection.
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| Molecular Formula |
C28H32NA2O8S2
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|---|---|
| Molecular Weight |
606.66
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| Exact Mass |
634.164
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| CAS # |
90458-75-6
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| PubChem CID |
77985954
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
40
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| Complexity |
1200
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1(C\\2CCC1(C(=O)/C2=C/C3=CC=C(C=C3)/C=C\\4/C5CCC(C4=O)(C5(C)C)CS(=O)(=O)[O-])CS(=O)(=O)[O-])C.[Na+].[Na+]
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| InChi Key |
ABZNEHKRFLKURR-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C28H34O8S2.2Na/c1-25(2)21-9-11-27(25,15-37(31,32)33)23(29)19(21)13-17-5-7-18(8-6-17)14-20-22-10-12-28(24(20)30,26(22,3)4)16-38(34,35)36;;/h5-8,13-14,21-22H,9-12,15-16H2,1-4H3,(H,31,32,33)(H,34,35,36);;/q;2*+1/p-2
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| Chemical Name |
disodium;[3-[[4-[[7,7-dimethyl-3-oxo-4-(sulfonatomethyl)-2-bicyclo[2.2.1]heptanylidene]methyl]phenyl]methylidene]-7,7-dimethyl-2-oxo-1-bicyclo[2.2.1]heptanyl]methanesulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 50 mg/mL (82.42 mM)
DMSO: 50 mg/mL (82.42 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.12 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.12 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (4.12 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6484 mL | 8.2418 mL | 16.4837 mL | |
| 5 mM | 0.3297 mL | 1.6484 mL | 3.2967 mL | |
| 10 mM | 0.1648 mL | 0.8242 mL | 1.6484 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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