| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
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| 250g |
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| Other Sizes |
| Targets |
Bis-Tris does not have a defined pharmacological target; it is a biological buffer. Its mechanism of action is purely chemical, functioning to resist changes in pH when acids or bases are added. It is often used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.
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|---|---|
| ln Vitro |
In vitro, Bis-Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations, and diagnostic reagent formulations. It is a component of transfer buffer for Western blotting and is suitable for column chromatography of proteins. It is also used as a process aid in peptide synthesis and as a scavenger agent for metal ions such as Cu and Pb.
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| ln Vivo |
In vivo data for Bis-Tris as a therapeutic agent are not applicable. Its primary role is as an in vitro reagent for maintaining pH in biochemical experiments. It is not administered for therapeutic purposes and has no established in vivo profile.
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| Enzyme Assay |
For in vitro biochemical assays, Bis-Tris is used as a buffer component. A typical protocol involves preparing a Bis-Tris buffer solution at a desired concentration (e.g., 10-100 mM) and adjusting the pH to the required value (e.g., pH 6.0-7.0). This buffer is then used in applications such as electrophoresis, Western blotting, or as a component in media formulations.
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| Cell Assay |
For in vitro cell-based experiments, Bis-Tris is used as a component of cell culture media to maintain pH. A standard protocol involves preparing a media solution containing Bis-Tris at a concentration of 10-50 mM and using it to culture cells. The buffer helps maintain a stable pH, which is crucial for cell viability and experimental reproducibility.
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| Animal Protocol |
In vivo animal studies for Bis-Tris are not applicable, as the compound is a buffer reagent used exclusively for in vitro applications. No animal studies have been conducted for this compound as a therapeutic agent.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Bis-Tris are not available, as the compound is not a drug and is not administered to living organisms. It is a zwitterionic buffer with a pKa of 6.5 at 25°C, designed for in vitro use to maintain pH in the range of 5.8-7.2. It is soluble in water up to 500 mg/mL.
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| Toxicity/Toxicokinetics |
Bis-Tris is generally considered to be a low-toxicity buffer reagent. It is classified as an eye irritant (Eye Irrit. 2). Standard laboratory safety practices should be followed, including the use of personal protective equipment.
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| References | |
| Additional Infomation |
Bis-Tris is a buffer substance used for calibrating glass electrodes and preparing biochemical and biological buffer solutions; its pKa value at 25 °C is 6.46. Its buffering properties are related to a member of the Tris family.
Bis-Tris (CAS 6976-37-0) is a research-grade biochemical buffer, not an FDA-approved pharmaceutical drug. Its primary application is as a zwitterionic buffering agent for pH stabilization in biochemical and biological research, including electrophoresis, Western blotting, and cell culture. |
| Molecular Formula |
C8H19NO5
|
|---|---|
| Molecular Weight |
209.24
|
| Exact Mass |
209.126
|
| CAS # |
6976-37-0
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| Related CAS # |
Bis-Tris-d19;352534-93-1
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| PubChem CID |
81462
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| Appearance |
White to off-white solid powder
|
| Density |
1.3±0.1 g/cm3
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| Boiling Point |
466.2±40.0 °C at 760 mmHg
|
| Melting Point |
104 °C(lit.)
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| Flash Point |
288.0±26.0 °C
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| Vapour Pressure |
0.0±2.6 mmHg at 25°C
|
| Index of Refraction |
1.563
|
| LogP |
-0.67
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
8
|
| Heavy Atom Count |
14
|
| Complexity |
125
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C(CO)N(CCO)C(CO)(CO)CO
|
| InChi Key |
OWMVSZAMULFTJU-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
|
| Chemical Name |
2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 250 mg/mL (1194.80 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (238.96 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7792 mL | 23.8960 mL | 47.7920 mL | |
| 5 mM | 0.9558 mL | 4.7792 mL | 9.5584 mL | |
| 10 mM | 0.4779 mL | 2.3896 mL | 4.7792 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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