| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Z-Glu-Tyr-OH does not have a specific biological target but is known for its role in protease enzyme studies. It is pivotal in understanding enzyme-substrate interactions, particularly in exploring the specificity of peptide bond cleavage by proteases. The compound's stable structure allows for detailed analysis in biochemical research, especially in laboratories focusing on peptide synthesis and enzymatic activity assays.
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|---|---|
| ln Vitro |
In vitro, Z-Glu-Tyr-OH is utilized as a substrate in enzymatic assays to study protease specificity and activity. Its cleavage pattern by enzymes such as acid proteinase A from Aspergillus niger has been investigated, revealing specific cleavage at Tyr-X and Glu-X bonds. This makes it a valuable tool for characterizing the substrate specificity of various proteases.
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| ln Vivo |
Z-Glu-Tyr-OH is not administered in vivo as a therapeutic agent. It is a research chemical used exclusively as a building block and substrate in biochemical assays. The compound is for research and manufacturing use only and is not intended for human or veterinary therapeutic applications.
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| Enzyme Assay |
In vitro enzyme assays using Z-Glu-Tyr-OH typically involve incubating the dipeptide with a target protease under controlled conditions (e.g., specific pH and temperature). The cleavage products are then analyzed by techniques such as HPLC to determine the specificity and efficiency of peptide bond hydrolysis. This allows researchers to map the substrate preferences of proteases.
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| Cell Assay |
Z-Glu-Tyr-OH is not used in cell-based assays as a therapeutic agent. Its applications are in synthetic chemistry and in vitro enzymology. It is typically stored as a solid at -20°C, tightly sealed and desiccated. The product is for research purposes only and is not intended for human or veterinary use.
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| Animal Protocol |
In vivo animal experiments do not involve Z-Glu-Tyr-OH as a test article. It is a chemical tool used in biochemical research to study protease function. The compound itself is never administered to animals as a therapeutic or test compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to Z-Glu-Tyr-OH as it is not a drug. It is a small molecule (MW 444.4 g/mol) with the formula C22H24N2O8. Its properties are relevant for chemical handling and storage rather than for systemic exposure or ADME studies.
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| Toxicity/Toxicokinetics |
The toxicity of Z-Glu-Tyr-OH is not extensively documented, as it is a research chemical not intended for human or veterinary use. Standard laboratory safety precautions should be followed when handling this compound, including the use of appropriate personal protective equipment.
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| References | |
| Additional Infomation |
Z-Glu-Tyr-OH is a synthetic dipeptide used as a substrate in protease enzyme studies. Its cleavage specificity has been investigated using enzymes such as acid proteinase A, revealing preferential cleavage at Tyr-X and Glu-X bonds. This compound is not a pharmaceutical and has no clinical trials or approved therapeutic status.
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| Molecular Formula |
C22H24N2O8
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|---|---|
| Molecular Weight |
444.43
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| Exact Mass |
444.153
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| CAS # |
988-75-0
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| PubChem CID |
96820
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| Appearance |
White to off-white solid powder
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| Density |
1.381g/cm3
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| Boiling Point |
815.3ºC at 760 mmHg
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| Melting Point |
185-187ºC
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| Flash Point |
446.9ºC
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| Index of Refraction |
1.608
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| LogP |
2.445
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
32
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| Complexity |
642
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| Defined Atom Stereocenter Count |
2
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| SMILES |
O=C(O)CC[C@@H](C(N[C@H](C(O)=O)CC1=CC=C(O)C=C1)=O)NC(OCC2=CC=CC=C2)=O
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| InChi Key |
XLUMOZQZGPJGTL-ROUUACIJSA-N
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| InChi Code |
InChI=1S/C22H24N2O8/c25-16-8-6-14(7-9-16)12-18(21(29)30)23-20(28)17(10-11-19(26)27)24-22(31)32-13-15-4-2-1-3-5-15/h1-9,17-18,25H,10-13H2,(H,23,28)(H,24,31)(H,26,27)(H,29,30)/t17-,18-/m0/s1
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| Chemical Name |
(4S)-5-[[(1S)-1-carboxy-2-(4-hydroxyphenyl)ethyl]amino]-5-oxo-4-(phenylmethoxycarbonylamino)pentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (225.01 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.63 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.63 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.63 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2501 mL | 11.2504 mL | 22.5007 mL | |
| 5 mM | 0.4500 mL | 2.2501 mL | 4.5001 mL | |
| 10 mM | 0.2250 mL | 1.1250 mL | 2.2501 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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