| Size | Price | |
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| Other Sizes |
| Targets |
As an amino acid derivative, N-Acetyl-D-methionine does not have a defined primary drug target. Its role is as a synthetic intermediate in peptide synthesis and as a research tool in metabolic studies. The acetylated methionine residue can influence the properties of peptides. However, the building block itself is not known to directly bind to or modulate any specific protein.
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|---|---|
| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
In vitro activity data for N-Acetyl-D-methionine is primarily related to its role as a chemical precursor and a research tool. It does not exhibit significant intrinsic pharmacological activity in cell-free or cell-based assays. The compound is used in vitro to synthesize peptides that are then evaluated for biological activity. It may also be used in enzyme assays to study the activity of enzymes that recognize acetylated amino acids. Amino acid derivatives like this one have been commercially used as ergogenic supplements, but this is not its primary application. |
| ln Vivo |
There is no reported in vivo activity for N-Acetyl-D-methionine as a drug substance. The compound is used primarily as a research chemical and a synthetic intermediate. It may be used in animal studies to investigate methionine metabolism or the effects of acetylation. However, it is not administered for pharmacological evaluation as a drug candidate. Its role is confined to the laboratory.
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| Enzyme Assay |
In vitro enzyme assays could use N-Acetyl-D-methionine as a substrate for enzymes that recognize acetylated amino acids, such as deacetylases. In such an assay, the compound is incubated with the enzyme, and the release of acetate or methionine is monitored using HPLC or a colorimetric method. This assay is commonly used to study the activity of histone deacetylases or other deacetylases.
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| Cell Assay |
Cell-based assays for N-Acetyl-D-methionine could involve studying its effects on cellular methionine metabolism or protein acetylation. For example, cultured cells could be treated with the compound, and cellular methionine or acetylated protein levels could be measured. This assay would help elucidate the role of acetylated methionine in cellular processes.
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| Animal Protocol |
In vivo animal experiments for N-Acetyl-D-methionine could involve studying its effects on methionine metabolism or its potential as a nutritional supplement. For example, rats could be administered the compound orally or intravenously, and blood levels of methionine and its metabolites could be measured over time.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of N-Acetyl-D-methionine have been studied to some extent. As an N-acetylated amino acid, it is expected to be absorbed and metabolized differently than methionine. It may be deacetylated to release methionine. However, detailed ADME data are not extensively available.
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| Toxicity/Toxicokinetics |
The toxicity profile of N-Acetyl-D-methionine is generally considered to be low, as it is a derivative of a naturally occurring amino acid. The compound is classified for research use only and is not intended for human or veterinary use. Standard laboratory safety precautions should be followed. Specific toxicological information is not extensively available.
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| References | |
| Additional Infomation |
N-acetyl-D-methionine is an N-acetyl-D-amino acid, with D-methionine as the amino acid. It is a derivative of D-methionine, and also an N-acetyl-D-amino acid and N-acetylmethionine. It is the conjugate acid of N-acetyl-D-methionine (1-) and an enantiomer of N-acetyl-L-methionine. N-acetyl-D-methionine is a metabolite found or produced in Saccharomyces cerevisiae.
N-Acetyl-D-methionine is an acetylated methionine derivative used in peptide synthesis and metabolic studies. It is not a drug and has no approved therapeutic indications. The compound is commercially available for research purposes only. |
| Molecular Formula |
C7H13NO3S
|
|---|---|
| Molecular Weight |
191.25
|
| Exact Mass |
191.061
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| CAS # |
1509-92-8
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| Related CAS # |
N-Acetyl-D-methionine-d4
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| PubChem CID |
6991987
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| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
453.6±40.0 °C at 760 mmHg
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| Melting Point |
117-119ºC
|
| Flash Point |
228.1±27.3 °C
|
| Vapour Pressure |
0.0±2.4 mmHg at 25°C
|
| Index of Refraction |
1.511
|
| LogP |
-0.19
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
12
|
| Complexity |
172
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
CC(N[C@@H](C(O)=O)CCSC)=O
|
| InChi Key |
XUYPXLNMDZIRQH-ZCFIWIBFSA-N
|
| InChi Code |
InChI=1S/C7H13NO3S/c1-5(9)8-6(7(10)11)3-4-12-2/h6H,3-4H2,1-2H3,(H,8,9)(H,10,11)/t6-/m1/s1
|
| Chemical Name |
(2R)-2-acetamido-4-methylsulfanylbutanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 100 mg/mL (522.88 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.07 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.07 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (13.07 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2288 mL | 26.1438 mL | 52.2876 mL | |
| 5 mM | 1.0458 mL | 5.2288 mL | 10.4575 mL | |
| 10 mM | 0.5229 mL | 2.6144 mL | 5.2288 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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