| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
Biotin-LC-LC-NHS targets primary amines (-NH₂) on proteins and other biomolecules through its NHS ester group, enabling covalent biotinylation. The biotin moiety then serves as a high-affinity ligand for avidin, streptavidin, and neutravidin proteins.
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|---|---|
| ln Vitro |
The process of biotinylating antibodies involves reacting with the protein in its aqueous phase at pH 7.4 using Biotin-LC-LC-NHS (1 mg/mL in dimethyl formamide). In the labeling reaction, the molar ratio of biotin to antibody was 10:1, with a final concentration of 70 μM of biotin [1].
The in vitro activity is characterized by its ability to biotinylate amine-containing biomolecules efficiently. The two LC spacers provide an extended linker (total length approximately 30.5 Å) that reduces steric hindrance and enhances the binding of biotinylated molecules to streptavidin or avidin. |
| ln Vivo |
Biotin-LC-LC-NHS is not used as a therapeutic agent. Its utility is in research applications for labeling, purification, and detection of biomolecules via the biotin-streptavidin interaction.
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| Enzyme Assay |
In vitro labeling involves incubating the NHS ester with amine-containing biomolecules at pH 7-9 to form stable amide bonds. The biotinylated product can be detected or purified using streptavidin-conjugated reagents.
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| Cell Assay |
For in vitro cellular experiments, proteins or antibodies are first biotinylated using Biotin-LC-LC-NHS. The labeled molecules are then used in various assays, such as ELISA, western blotting, or immunoprecipitation, where they are detected or captured using streptavidin.
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| Animal Protocol |
In vivo animal experiments are not typically performed with Biotin-LC-LC-NHS itself. However, biotinylated molecules prepared with this reagent can be administered to animals for tracking or targeting studies using streptavidin-based detection systems.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Biotin-LC-LC-NHS have not been characterized. The compound has a molecular formula of C₃₂H₅₃N₅O₉S and a molecular weight of 683.86 g/mol. It is moisture-sensitive and should be stored desiccated at -20°C.
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| Toxicity/Toxicokinetics |
Toxicological data for Biotin-LC-LC-NHS has not been systematically evaluated, as it is intended for research applications only. Standard laboratory safety precautions should be observed.
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| References | |
| Additional Infomation |
Biotin-LC-LC-NHS is an amine-reactive biotinylation reagent with two long-chain spacers for reducing steric hindrance and improving accessibility for avidin or streptavidin binding.
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| Molecular Formula |
C26H41N5O7S
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|---|---|
| Molecular Weight |
567.70
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| Exact Mass |
567.272
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| CAS # |
89889-52-1
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| PubChem CID |
16219024
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.3±0.1 g/cm3
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| Melting Point |
147-151ºC
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| Index of Refraction |
1.579
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| LogP |
-1.83
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
19
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| Heavy Atom Count |
39
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| Complexity |
886
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCC[C@H]2[C@@H]3[C@H](CS2)NC(=O)N3
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| InChi Key |
ATYCFNRXENKXSE-MHPIHPPYSA-N
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| InChi Code |
InChI=1S/C26H41N5O7S/c32-20(27-16-8-2-4-12-24(36)38-31-22(34)13-14-23(31)35)10-3-1-7-15-28-21(33)11-6-5-9-19-25-18(17-39-19)29-26(37)30-25/h18-19,25H,1-17H2,(H,27,32)(H,28,33)(H2,29,30,37)/t18-,19-,25-/m0/s1
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 6-[6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoylamino]hexanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 125 mg/mL (220.19 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.66 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.66 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (3.66 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7615 mL | 8.8075 mL | 17.6149 mL | |
| 5 mM | 0.3523 mL | 1.7615 mL | 3.5230 mL | |
| 10 mM | 0.1761 mL | 0.8807 mL | 1.7615 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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