| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
2-(2′-Hydroxyphenyl)benzimidazole does not target a specific protein or enzyme. Its utility as a fluorescent probe is based on its ESIPT properties, which make it sensitive to the local environment and proton transfer reactions.
|
|---|---|
| ln Vitro |
The in vitro activity of 2-(2′-Hydroxyphenyl)benzimidazole is characterized by its ESIPT fluorescence, which results in a large Stokes shift and sensitivity to the local environment. The compound is used as a fluorescent probe for studying proton transfer reactions and as a pH indicator.
|
| ln Vivo |
2-(2′-Hydroxyphenyl)benzimidazole is not used as a therapeutic agent. Its in vivo utility is limited to research applications as a fluorescent probe. The compound is not intended for systemic therapeutic use.
|
| Enzyme Assay |
In vitro assays for 2-(2′-Hydroxyphenyl)benzimidazole involve measuring its fluorescence properties under various conditions to study ESIPT mechanisms and proton transfer reactions. The compound can be used as a fluorescent probe to investigate molecular interactions and environmental effects.
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| Cell Assay |
For in vitro cellular experiments, 2-(2′-Hydroxyphenyl)benzimidazole may be used as a fluorescent probe for imaging applications. Cells are incubated with the compound and analyzed by fluorescence microscopy.
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| Animal Protocol |
In vivo animal experiments with 2-(2′-Hydroxyphenyl)benzimidazole are not typically performed, as the compound is primarily used as a fluorescent probe for in vitro studies. The compound is intended for research use only.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 2-(2′-Hydroxyphenyl)benzimidazole have not been characterized, as the compound is a research-use fluorescent probe rather than a drug candidate. The compound has a molecular weight of 210.23 g/mol and should be stored under appropriate conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for 2-(2′-Hydroxyphenyl)benzimidazole has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed.
|
| References | |
| Additional Infomation |
Structure in the first source
2-(2′-Hydroxyphenyl)benzimidazole is a fluorescent compound exhibiting ESIPT fluorescence, used as a fluorescent probe for studying proton transfer reactions and as a pH indicator. The compound has no clinical or therapeutic applications. |
| Molecular Formula |
C13H10N2O
|
|---|---|
| Molecular Weight |
210.23
|
| Exact Mass |
210.079
|
| CAS # |
2963-66-8
|
| PubChem CID |
18075
|
| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.289g/cm3
|
| Boiling Point |
388.6ºC at 760mmHg
|
| Melting Point |
239-243ºC
|
| Flash Point |
167.6ºC
|
| Vapour Pressure |
0mmHg at 25°C
|
| Index of Refraction |
1.726
|
| LogP |
2.935
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
16
|
| Complexity |
246
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C(C(=C1)C2=NC3=CC=CC=C3N2)O
|
| InChi Key |
XWXMGTIHBYFTIE-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C13H10N2O/c16-12-8-4-1-5-9(12)13-14-10-6-2-3-7-11(10)15-13/h1-8,16H,(H,14,15)
|
| Chemical Name |
2-(1H-benzimidazol-2-yl)phenol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 100 mg/mL (475.67 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.89 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.89 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7567 mL | 23.7835 mL | 47.5670 mL | |
| 5 mM | 0.9513 mL | 4.7567 mL | 9.5134 mL | |
| 10 mM | 0.4757 mL | 2.3783 mL | 4.7567 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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