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|---|---|---|
| Other Sizes |
| Targets |
The carboxylic acid group on 7-Dimethylaminocoumarin-4-acetic acid provides a reactive site for conjugation to amines through carbodiimide-mediated coupling reactions. The compound does not target specific enzymes or receptors but serves as a fluorescent labeling reagent.
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| ln Vitro |
The in vitro activity of 7-Dimethylaminocoumarin-4-acetic acid is characterized by its strong blue fluorescence, with excitation and emission maxima around 395 nm and 470 nm, respectively. The dye is used as a fluorescent label for proteins, peptides, and other biomolecules. The dimethylamino group at the 7-position enhances the fluorescence properties of the coumarin core.
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| ln Vivo |
7-Dimethylaminocoumarin-4-acetic acid is not used as a therapeutic agent. Its in vivo utility is limited to research applications as a fluorescent labeling reagent. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro labeling assays for 7-Dimethylaminocoumarin-4-acetic acid involve converting the carboxylic acid to an NHS ester and incubating with amine-containing biomolecules. The NHS ester reacts with lysine residues to form photostable amide links. Labeling efficiency can be assessed by measuring the fluorescence intensity of the conjugated product.
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| Cell Assay |
For in vitro cellular experiments, 7-Dimethylaminocoumarin-4-acetic acid is conjugated to antibodies, proteins, or other targeting ligands via the carboxylic acid group or NHS ester. The labeled conjugates are then added to cell culture medium for incubation with target cells. After washing, cells are analyzed by fluorescence microscopy. The blue fluorescence provides distinct spectral properties.
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| Animal Protocol |
In vivo animal experiments with 7-Dimethylaminocoumarin-4-acetic acid are not typically performed, as the compound is primarily used as an in vitro labeling reagent. The compound is intended for research use only.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 7-Dimethylaminocoumarin-4-acetic acid have not been characterized, as the compound is a research-use fluorescent labeling reagent rather than a drug candidate. The compound has a molecular weight of 247.25 g/mol and should be stored under appropriate conditions, protected from light.
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| Toxicity/Toxicokinetics |
Toxicological data for 7-Dimethylaminocoumarin-4-acetic acid has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed.
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| References | |
| Additional Infomation |
7-Dimethylaminocoumarin-4-acetic acid is a coumarin-based fluorescent dye with blue fluorescence, used as a fluorescent label for proteins, peptides, and other biomolecules. The compound has no clinical or therapeutic applications.
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| Molecular Formula |
C13H13NO4
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|---|---|
| Molecular Weight |
247.25
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| Exact Mass |
247.084
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| CAS # |
80883-54-1
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| PubChem CID |
2762622
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| Appearance |
Light brown to brown solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
497.6±45.0 °C at 760 mmHg
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| Flash Point |
254.8±28.7 °C
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| Vapour Pressure |
0.0±1.3 mmHg at 25°C
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| Index of Refraction |
1.619
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| LogP |
2.24
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
18
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| Complexity |
389
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
CN(C)C1=CC2=C(C=C1)C(=CC(=O)O2)CC(=O)O
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| InChi Key |
HQMBLJOHUDYJLK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H13NO4/c1-14(2)9-3-4-10-8(5-12(15)16)6-13(17)18-11(10)7-9/h3-4,6-7H,5H2,1-2H3,(H,15,16)
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| Chemical Name |
2-[7-(dimethylamino)-2-oxochromen-4-yl]acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (404.45 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (10.11 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0445 mL | 20.2224 mL | 40.4449 mL | |
| 5 mM | 0.8089 mL | 4.0445 mL | 8.0890 mL | |
| 10 mM | 0.4044 mL | 2.0222 mL | 4.0445 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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