| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
The amine functional group on ICG-amine enables conjugation to carboxyl-containing biomolecules through carbodiimide-mediated coupling reactions. The compound does not target specific enzymes or receptors but serves as a labeling reagent for attaching the ICG fluorophore to biomolecules for NIR imaging applications.
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|---|---|
| ln Vitro |
ICG is a tricarbocyanine dye[1].
The in vitro activity of ICG-amine is characterized by its strong near-infrared fluorescence properties. The dye emits in the NIR region, providing deep tissue penetration and low background fluorescence, which is advantageous for in vitro and in vivo imaging applications. The amine group enables covalent attachment to proteins and antibodies. |
| ln Vivo |
ICG-amine is not used as a therapeutic agent. Its in vivo utility is as a labeling reagent for near-infrared imaging applications. When conjugated to targeting ligands, the dye enables non-invasive NIR imaging for tracking biodistribution and target engagement in animal models.
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| Enzyme Assay |
In vitro labeling assays for ICG-amine involve incubating the amine with carboxyl-containing biomolecules in the presence of carbodiimide coupling reagents. The reaction forms stable amide bonds between the amine and carboxyl groups. Labeling efficiency can be assessed by measuring the fluorescence intensity of the conjugated product.
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| Cell Assay |
For in vitro cellular experiments, ICG-amine is conjugated to antibodies, proteins, or other targeting ligands via the amine-carboxyl coupling reaction. The labeled conjugates are then added to cell culture medium for incubation with target cells. After washing, cells are analyzed by fluorescence microscopy or flow cytometry. The NIR fluorescence provides deep tissue penetration and low background.
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| Animal Protocol |
For in vivo animal experiments, ICG-amine is conjugated to targeting ligands and administered to animal models. The NIR emission provides deep tissue penetration and low background fluorescence, making it suitable for in vivo imaging studies. Conjugates bearing ICG-amine can be administered to animal models for tracking biodistribution and target engagement.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of ICG-amine have not been extensively characterized. The compound is a derivative of Indocyanine Green, which is clinically used for various diagnostic applications. ICG is cleared by the liver and excreted in bile. Standard PK studies would assess parameters such as half-life and biodistribution.
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| Toxicity/Toxicokinetics |
Toxicological data for ICG-amine has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. ICG itself has a well-established safety profile for clinical use. Standard laboratory safety precautions should be observed.
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| References | |
| Additional Infomation |
ICG-amine is an amine-functionalized derivative of Indocyanine Green (ICG), a near-infrared fluorescent dye used for medical diagnostics and imaging. The amine group enables conjugation to biomolecules for NIR imaging applications. The compound has no clinical or therapeutic applications and has not received regulatory approval.
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| Molecular Formula |
C51H64N4O4S
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|---|---|
| Molecular Weight |
829.1433
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| Exact Mass |
828.464
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| CAS # |
1686147-55-6
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| PubChem CID |
154728428
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| Appearance |
Green to dark green solid powder
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| LogP |
7.4
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
20
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| Heavy Atom Count |
60
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| Complexity |
1660
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(CCCCN1C2C=CC3=CC=CC=C3C=2C(C)(C)/C/1=C/C=C/C=C/C=C/C1C(C)(C)C2C3=CC=CC=C3C=CC=2[N+]=1CCCCCC(NCCCCCCN)=O)(=O)(=O)[O-]
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| InChi Key |
WKCQFHIAPMFCGN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C51H64N4O4S/c1-50(2)45(54(43-32-30-39-23-14-16-25-41(39)48(43)50)36-20-10-13-29-47(56)53-35-19-9-8-18-34-52)27-11-6-5-7-12-28-46-51(3,4)49-42-26-17-15-24-40(42)31-33-44(49)55(46)37-21-22-38-60(57,58)59/h5-7,11-12,14-17,23-28,30-33H,8-10,13,18-22,29,34-38,52H2,1-4H3,(H-,53,56,57,58,59)
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| Chemical Name |
4-[(2Z)-2-[(2E,4E,6E)-7-[3-[6-(6-aminohexylamino)-6-oxohexyl]-1,1-dimethylbenzo[e]indol-3-ium-2-yl]hepta-2,4,6-trienylidene]-1,1-dimethylbenzo[e]indol-3-yl]butane-1-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 31.25 mg/mL (37.69 mM)
H2O: 5 mg/mL (6.03 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (1.51 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2061 mL | 6.0303 mL | 12.0607 mL | |
| 5 mM | 0.2412 mL | 1.2061 mL | 2.4121 mL | |
| 10 mM | 0.1206 mL | 0.6030 mL | 1.2061 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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