| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside targets the enzymes N-acetyl-β-D-glucosaminidase and β-hexosaminidases. It serves as a fluorogenic substrate for these enzymes, releasing a fluorescent product upon enzymatic cleavage.
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|---|---|
| ln Vitro |
The capacity of the enzyme to extract N-acetylglucosamine and N-acetylgalactosamine residues bound by β-glycosidically from 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside is a measure of its functionality[1].
The in vitro activity of this compound is its function as a fluorogenic substrate for β-hexosaminidases. Upon enzymatic cleavage, 4-methylumbelliferone (4-MU) is released, and its fluorescence can be used to quantify β-hexosaminidase activity. The compound enables sensitive and quantitative enzyme activity analysis. |
| ln Vivo |
4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the measurement of β-hexosaminidase activity in biological samples. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro enzyme assays for this compound involve incubating the fluorogenic substrate with β-hexosaminidase enzyme preparations in appropriate buffer conditions. The enzyme cleaves the substrate, releasing 4-methylumbelliferone (4-MU), which is measured fluorimetrically. The fluorescence intensity is proportional to enzyme activity.
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| Cell Assay |
For in vitro cellular experiments, this compound is typically used in cell-free systems for enzyme activity assays. The substrate can be added to cell lysates or purified enzyme preparations to assess β-hexosaminidase activity. The fluorogenic readout provides a sensitive and quantitative measure of enzyme activity.
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| Animal Protocol |
In vivo animal experiments with this compound are not typically performed, as the compound is used as an in vitro enzyme substrate. However, the substrate can be used to measure β-hexosaminidase activity in samples collected from animal models.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of this compound have not been characterized, as it is a research-use enzyme substrate rather than a drug candidate. The compound has a molecular weight of 379.36 g/mol and a melting point of 212°C to 222°C. It should be stored under appropriate conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for this compound has not been systematically evaluated, as it is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside is the (β) isomer.
4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside is a fluorogenic substrate for N-acetyl-β-D-glucosaminidase and β-hexosaminidases. Upon enzymatic cleavage, 4-methylumbelliferone is released and its fluorescence quantifies enzyme activity. The compound has no clinical or therapeutic applications. |
| Molecular Formula |
C18H21NO8
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|---|---|
| Molecular Weight |
379.36
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| Exact Mass |
379.126
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| CAS # |
37067-30-4
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| PubChem CID |
2733787
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
732.2±60.0 °C at 760 mmHg
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| Melting Point |
208-210 °C
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| Flash Point |
396.6±32.9 °C
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| Vapour Pressure |
0.0±2.5 mmHg at 25°C
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| Index of Refraction |
1.632
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| LogP |
0.34
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
27
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| Complexity |
608
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| Defined Atom Stereocenter Count |
5
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| SMILES |
CC1=CC(=O)OC2=C1C=CC(=C2)O[C@H]3[C@@H]([C@H]([C@@H]([C@H](O3)CO)O)O)NC(=O)C
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| InChi Key |
QCTHLCFVVACBSA-JVNHZCFISA-N
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| InChi Code |
InChI=1S/C18H21NO8/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21)/t13-,15-,16-,17-,18-/m1/s1
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| Chemical Name |
N-[(2S,3R,4R,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-2-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]acetamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50 mg/mL (131.80 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.59 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.59 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.59 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6360 mL | 13.1801 mL | 26.3602 mL | |
| 5 mM | 0.5272 mL | 2.6360 mL | 5.2720 mL | |
| 10 mM | 0.2636 mL | 1.3180 mL | 2.6360 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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