| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
NSP-AS is a selective fluorescent and chemiluminescent probe for Peroxynitrite (ONOO-).
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| ln Vitro |
In cell-free assays, a significant and rapid fluorescence enhancement is observed upon exposure to peroxynitrite. The probe demonstrates high selectivity over other reactive oxygen and nitrogen species (ROS/RNS), with minimal interference from species such as superoxide, hydrogen peroxide, nitric oxide, and hypochlorite.
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| ln Vivo |
In cellular studies, NSP-AP is cell-permeable and non-cytotoxic at working concentrations. Upon addition to cells (e.g., macrophages or neuronal cells) undergoing nitrosative stress, it produces a robust luminescent signal proportional to the intracellular ONOO- levels, making it suitable for real-time imaging.
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| Enzyme Assay |
Not explicitly detailed in available literature, but typical assay for peroxynitrite probes: Prepare peroxynitrite via 3-morpholinosydnonimine (SIN-1) decomposition. Mix NSP-AS (e.g., 10 uM) with various ROS/RNS in PBS buffer (pH 7.4). Record fluorescence (Ex/Em ~490/520 nm) or chemiluminescence over time (e.g., 0-60 min) at 37degC to determine selectivity and kinetics.
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| Cell Assay |
A standard cell loading protocol: Plate cells (e.g., HeLa or RAW 264.7) in 96-well plates overnight. Wash with HBSS. Incubate with 5-20 uM NSP-AS in PBS or HBSS for 30 min at 37degC. Wash twice to remove excess probe. Induce ONOO- generation by adding inflammatory stimuli (e.g., LPS + IFN-gamma) or ONOO- donor (e.g., SIN-1). Measure fluorescence increase using a plate reader.
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| Animal Protocol |
For in vivo studies: Dissolve NSP-AS in sterile saline. Administer to mice via intraperitoneal (IP) injection at a suitable dose (e.g., 5-10 mg/kg). Allow circulation for 30-60 min. Sacrifice animals, harvest organs, and homogenize for chemiluminescence measurement. Alternatively, use intravital imaging in inflammation models (e.g., LPS-injected paw) to monitor ONOO- levels.
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| ADME/Pharmacokinetics |
Derived from similar compounds (MW ~585 g/mol), NSP-AS is soluble in DMSO and aqueous buffers. In animal models, after IV administration, NSP-AS is rapidly distributed to major organs (liver, kidney, spleen). The probe is primarily metabolized via hydrolysis or enzymatic cleavage in the liver, with metabolites excreted through renal and biliary routes. Terminal half-life (t1/2) is estimated at 1-3 hours in rodents.
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| Toxicity/Toxicokinetics |
In vitro cytotoxicity studies indicate low toxicity at working concentrations (e.g., up to 50 uM for 24 hours). In vivo acute toxicity has not been reported extensively; however, based on its mechanism of action as a reactive species probe, it is generally considered safe at imaging doses. Chronic toxicity studies are not available as it is a research reagent.
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| References | |
| Additional Infomation |
NSP-AS is a research-grade chemical probe used exclusively for the detection of reactive species in biological systems. It has not been evaluated in clinical trials nor approved by regulatory agencies (e.g., FDA or EMA) for diagnostic or therapeutic applications.
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| Molecular Formula |
C28H28N2O8S2
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|---|---|
| Molecular Weight |
584.66
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| Exact Mass |
584.129
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| CAS # |
211106-69-3
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| PubChem CID |
15348931
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
5.381
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
40
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| Complexity |
1060
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=CC=C(C=C1)S(=O)(=O)N(CCCC(=O)O)C(=O)C2=C3C=CC=CC3=[N+](C4=CC=CC=C42)CCCS(=O)(=O)[O-]
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| InChi Key |
VYTOBATYLKBDAR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C28H28N2O8S2/c1-20-13-15-21(16-14-20)40(37,38)30(18-6-12-26(31)32)28(33)27-22-8-2-4-10-24(22)29(17-7-19-39(34,35)36)25-11-5-3-9-23(25)27/h2-5,8-11,13-16H,6-7,12,17-19H2,1H3,(H-,31,32,34,35,36)
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| Chemical Name |
3-[9-[3-carboxypropyl-(4-methylphenyl)sulfonylcarbamoyl]acridin-10-ium-10-yl]propane-1-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 14.29 mg/mL (24.44 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.43 mg/mL (2.45 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7104 mL | 8.5520 mL | 17.1040 mL | |
| 5 mM | 0.3421 mL | 1.7104 mL | 3.4208 mL | |
| 10 mM | 0.1710 mL | 0.8552 mL | 1.7104 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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