| Size | Price | |
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| Other Sizes |
| Targets |
4-Nitrophenyl β-D-xylopyranoside targets the enzyme β-xylosidase (β-D-xylan xylohydrolase), serving as a chromogenic substrate for the assessment of enzyme activity. The compound is specifically designed to be cleaved by this enzyme, releasing a chromophore that can be detected spectrophotometrically.
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|---|---|
| ln Vitro |
At high concentrations of 4-Nitrophenyl β-D-xylopyranoside and with xylo-oligosaccharides, β-D-xylan xylohydrolase demonstrates glycosyltransferase activity[1].
The in vitro activity of 4-Nitrophenyl β-D-xylopyranoside is its function as a chromogenic substrate for β-xylosidase. Upon enzymatic cleavage, the released 4-nitrophenol can be quantified by colorimetric detection at 405 nm. The compound facilitates the assessment of β-xylosidase activity in various biological samples. |
| ln Vivo |
4-Nitrophenyl β-D-xylopyranoside is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the measurement of β-xylosidase activity in biological samples. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro enzyme assays for 4-Nitrophenyl β-D-xylopyranoside involve incubating the chromogenic substrate with β-xylosidase enzyme preparations in appropriate buffer conditions. The enzyme cleaves the substrate, releasing 4-nitrophenol, which is measured spectrophotometrically at 405 nm. The rate of 4-nitrophenol release is proportional to β-xylosidase activity.
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| Cell Assay |
For in vitro cellular experiments, 4-Nitrophenyl β-D-xylopyranoside is typically used in cell-free systems for enzyme activity assays. The substrate can be added to cell lysates or purified enzyme preparations to assess β-xylosidase activity. The chromogenic readout provides a simple and quantitative measure of enzyme activity.
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| Animal Protocol |
In vivo animal experiments with 4-Nitrophenyl β-D-xylopyranoside are not typically performed, as the compound is used as an in vitro enzyme substrate. However, the substrate can be used to measure β-xylosidase activity in samples collected from animal models.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 4-Nitrophenyl β-D-xylopyranoside have not been characterized, as the compound is a research-use enzyme substrate rather than a drug candidate. The compound has a molecular weight of 271.22 g/mol and a melting point of 158.0 to 162.0 °C. It should be stored under appropriate conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for 4-Nitrophenyl β-D-xylopyranoside has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
4-Nitrophenyl β-D-xyloside is a xyloside, a derivative of β-D-xylanose, in which the terminal hydroxyl hydrogen is replaced by a 4-nitrophenyl group. It is a chromogenic compound. It is a xyloside and also a C-nitro compound. Functionally, it is related to 4-nitrophenol.
4-Nitrophenyl β-D-xylopyranoside is a chromogenic substrate for β-xylosidase, facilitating the assessment of enzyme activity. Upon hydrolysis by β-xylosidase, 4-nitrophenol is released and quantified by colorimetric detection at 405 nm. The compound has no clinical or therapeutic applications and has not received regulatory approval. |
| Molecular Formula |
C11H13NO7
|
|---|---|
| Molecular Weight |
271.22
|
| Exact Mass |
271.069
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| CAS # |
2001-96-9
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| PubChem CID |
91509
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| Appearance |
White to light yellow solid powder
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| Density |
1.597g/cm3
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| Boiling Point |
523.2ºC at 760 mmHg
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| Melting Point |
160 °C
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| Flash Point |
270.2ºC
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| Vapour Pressure |
8.94E-12mmHg at 25°C
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| Index of Refraction |
1.654
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| LogP |
-0.3
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
2
|
| Heavy Atom Count |
19
|
| Complexity |
313
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
C1[C@H]([C@@H]([C@H]([C@@H](O1)OC2=CC=C(C=C2)[N+](=O)[O-])O)O)O
|
| InChi Key |
MLJYKRYCCUGBBV-YTWAJWBKSA-N
|
| InChi Code |
InChI=1S/C11H13NO7/c13-8-5-18-11(10(15)9(8)14)19-7-3-1-6(2-4-7)12(16)17/h1-4,8-11,13-15H,5H2/t8-,9+,10-,11+/m1/s1
|
| Chemical Name |
(2S,3R,4S,5R)-2-(4-nitrophenoxy)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (368.70 mM)
H2O: 50 mg/mL (184.35 mM） |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.22 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.22 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.22 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6870 mL | 18.4352 mL | 36.8704 mL | |
| 5 mM | 0.7374 mL | 3.6870 mL | 7.3741 mL | |
| 10 mM | 0.3687 mL | 1.8435 mL | 3.6870 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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