| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Targets |
Nitric oxide (NO) is the primary molecular target. The probe is designed to specifically detect NO via an OPD-based reaction mechanism.
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| ln Vitro |
RB-OPD (compound 1) can be used to detect NO in aqueous solution under physiologically normal conditions due to its "turn-on" type fluorogenic and chromogenic behavior toward NO. Its sensitivity and selectivity are quite high. In order to function as a "masked" NO-sensitive modulator, o-phenylenediamine, a NO-reactive group in lactam form, and rhodamine B spirolactam, a possible strong fluorophore and chromophore, make up NO-red.
RB-OPD (NO-red) is an o-phenylenediamine (OPD) type nitric oxide (NO) fluorescent probe with high sensitivity and selectivity (λex=550 nm, λem=590 nm). The probe remains non-fluorescent until it reacts with NO to form a triazole derivative, turning on fluorescence. |
| ln Vivo |
There is no specific in vivo activity data available for this compound. However, as a fluorescent probe for NO, similar compounds are typically used for real-time imaging of NO in live cells and animal models.
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| Enzyme Assay |
A standard protocol involves incubating RB-OPD (e.g., 5-10 uM) with varying concentrations of an NO donor (e.g., SNAP) in a buffer solution (e.g., PBS, pH 7.4) for 30-60 minutes at room temperature. The fluorescence intensity (Ex/Em: 550/590 nm) is then measured with a fluorescence spectrophotometer.
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| Cell Assay |
A general protocol for cellular imaging involves seeding cells in a confocal dish and incubating with RB-OPD (e.g., 1-10 uM) for 30-60 minutes at 37degC. Cells are then washed and treated with an NO donor to induce NO production. Fluorescence imaging is performed using a confocal microscope with a TRITC filter set.
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| Animal Protocol |
A typical protocol for in vivo imaging involves intravenous or intraperitoneal injection of an optimized concentration of RB-OPD into a live animal model. After a suitable circulation time (e.g., 30 minutes to 2 hours), the animal is placed under an in vivo imaging system to monitor NO-induced fluorescence.
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| ADME/Pharmacokinetics |
General PK properties for small molecule probes are not applicable, as these compounds are typically used for acute imaging. However, formulation can be in PBS or saline with co-solvents like DMSO for in vivo use.
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| Toxicity/Toxicokinetics |
General toxicity for imaging probes is considered low. For RB-OPD, no specific toxicity studies are reported. In similar experiments, incubation with up to 10 uM for 24 hours in cell models shows good biocompatibility and no significant cytotoxicity.
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| References | |
| Additional Infomation |
RB-OPD is a research tool for studying the role of NO in various physiological and pathological processes, including vasodilation, neurotransmission, and inflammation. The OPD-based lock mechanism ensures high selectivity over other reactive nitrogen species. This product is for research use only.
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| Molecular Formula |
C34H36N4O2
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|---|---|
| Molecular Weight |
532.68
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| Exact Mass |
532.283
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| CAS # |
1034863-51-8
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| PubChem CID |
102406797
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
6.3
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
40
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| Complexity |
838
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1C=CC=C2C=1C(=O)N(C1C=CC=CC=1N)C12C2C(OC3C1=CC=C(C=3)N(CC)CC)=CC(=CC=2)N(CC)CC
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| InChi Key |
FLFCPVCPMCLBEW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C34H36N4O2/c1-5-36(6-2)23-17-19-27-31(21-23)40-32-22-24(37(7-3)8-4)18-20-28(32)34(27)26-14-10-9-13-25(26)33(39)38(34)30-16-12-11-15-29(30)35/h9-22H,5-8,35H2,1-4H3
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| Chemical Name |
2-(2-aminophenyl)-3',6'-bis(diethylamino)spiro[isoindole-3,9'-xanthene]-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 25 mg/mL (46.93 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (4.69 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8773 mL | 9.3865 mL | 18.7730 mL | |
| 5 mM | 0.3755 mL | 1.8773 mL | 3.7546 mL | |
| 10 mM | 0.1877 mL | 0.9386 mL | 1.8773 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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