| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Hypochlorous acid (HOCl) is the primary molecular target. The probe is designed for the selective detection of HOCl over other reactive oxygen species (ROS).
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|---|---|
| ln Vitro |
In an aqueous solution with a large Stokes shift (~170 nm), EtS-DMAB can selectively detect hypochlorous acid (HOCl) over other reactive oxygen species. As it turns out, the turn-on fluorescence is caused by the thioether being oxidized to the corresponding sulfoxide[1].
EtS-DMAB (HClO-green) is a fluorescent probe, which can selectively detect hypochlorous acid (HOCl) (λex=440 nm, λem=610 nm). EtS-DMAB is applied to image exogenous and endogenous HOCl in live cells. |
| ln Vivo |
There is no specific in vivo activity data available for this compound. However, as a fluorescent probe for reactive species, similar compounds are typically used for real-time imaging of HOCl in live cells and animal models.
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| Enzyme Assay |
A standard protocol involves incubating EtS-DMAB (e.g., 5 uM) with varying concentrations of HOCl in a buffer solution (e.g., PBS, pH 7.4) for a short period (e.g., 5-10 minutes) at room temperature. The fluorescence intensity (Ex/Em: 440/610 nm) is then measured with a fluorescence spectrophotometer.
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| Cell Assay |
A general protocol for cellular imaging involves seeding cells in a confocal dish and incubating with EtS-DMAB (e.g., 1-10 uM) for 20-30 minutes at 37degC. Cells are then washed to remove excess probe. Fluorescence imaging is performed with a confocal microscope using excitation at 440 nm and emission collection at 610 nm.
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| Animal Protocol |
A typical protocol for in vivo imaging involves intravenous or intraperitoneal injection of an optimized concentration of EtS-DMAB into a live animal model. After a suitable circulation time (e.g., 30 minutes to 2 hours), the animal is placed under an in vivo imaging system to monitor HOCl-induced fluorescence.
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| ADME/Pharmacokinetics |
General PK properties for small molecule probes are not applicable, as these compounds are typically used for acute imaging. However, formulation can be in PBS or saline with co-solvents like DMSO for in vivo use.
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| Toxicity/Toxicokinetics |
General toxicity for imaging probes is considered low. For EtS-DMAB, no specific toxicity studies are reported. In similar experiments, incubation with up to 10 uM for 24 hours in cell models shows good biocompatibility and no significant cytotoxicity.
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| References | |
| Additional Infomation |
EtS-DMAB is a research tool for studying oxidative stress and inflammatory diseases. The large Stokes shift (440/610 nm) helps minimize self-quenching and autofluorescence interference, providing high-contrast imaging of HOCl. This product is for research use only.
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| Molecular Formula |
C10H13N3OS
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|---|---|
| Molecular Weight |
223.294720411301
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| Exact Mass |
223.077
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| CAS # |
2929446-76-2
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| PubChem CID |
162642082
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| Appearance |
Brown to reddish brown viscous liquid
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| LogP |
2.2
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
15
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| Complexity |
215
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(CC)C1=CC=C(C2C1=NON=2)N(C)C
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| InChi Key |
AEMFWHVETFLRKQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H13N3OS/c1-4-15-8-6-5-7(13(2)3)9-10(8)12-14-11-9/h5-6H,4H2,1-3H3
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| Chemical Name |
4-ethylsulfanyl-N,N-dimethyl-2,1,3-benzoxadiazol-7-amine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50 mg/mL (223.92 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4785 mL | 22.3924 mL | 44.7848 mL | |
| 5 mM | 0.8957 mL | 4.4785 mL | 8.9570 mL | |
| 10 mM | 0.4478 mL | 2.2392 mL | 4.4785 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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