| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Methyl Blue does not target a specific protein or enzyme. Its utility in biological staining relies on its physical and chemical properties as a dye rather than binding to a specific molecular target. The dye's interaction with biological tissues is based on electrostatic and hydrophobic interactions with cellular components.
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|---|---|
| ln Vitro |
The in vitro activity of Methyl Blue is characterized by its strong absorbance maximum at 594-610 nm in acidic conditions. As a dye, its primary activity is the ability to stain biological tissues, particularly collagen and connective tissue components. It is certified for use in Mallory's connective tissue stain and as a substitute for Fast Green FCF in Gomori's one-step trichrome procedure.
|
| ln Vivo |
Methyl Blue has demonstrated in vivo antimicrobial properties, functioning as an antiseptic agent. However, it is not used as a therapeutic agent but rather as a diagnostic staining reagent. The compound is not intended for systemic therapeutic use.
|
| Enzyme Assay |
In vitro binding assays are not applicable for Methyl Blue as it functions as a histological dye rather than a drug targeting specific enzymes or receptors. The compound is employed in staining protocols where it is applied directly to tissue sections or microbiological samples to provide contrast for microscopic visualization.
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| Cell Assay |
For in vitro cellular experiments, Methyl Blue is used in histological and microbiological staining procedures. Tissue sections or microbial samples are incubated with the dye solution, and after appropriate washing steps, the stained samples are examined under a microscope. The dye provides contrast for the visualization of cellular and tissue structures.
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| Animal Protocol |
In vivo animal experiments with Methyl Blue are not typically performed for therapeutic purposes. The compound is used as a diagnostic dye in research settings. Its use in animals is limited to staining applications in histological analysis of tissue samples.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Methyl Blue have not been characterized, as the compound is a research-use dye rather than a drug candidate. The compound is soluble in cold and hot water, forming a blue solution. It should be stored under appropriate conditions to maintain stability.
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| Toxicity/Toxicokinetics |
Toxicological data for Methyl Blue indicates that it is considered a xenobiotic compound. While it has antiseptic properties, its systemic toxicity has not been systematically evaluated. As with all chemical reagents, standard laboratory safety precautions should be observed during handling. The compound is not intended for human therapeutic use.
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| References |
|
| Additional Infomation |
See also: Aztec Blue (note moved to).
Methyl Blue (C.I. 42780) is a triaminotriphenylmethane dye with antiseptic properties, widely used in histology and microbiology for polychromatic staining. It is a CE certified IVD product for diagnostic staining of human tissue sections. The compound has no clinical or therapeutic applications and has not received regulatory approval for systemic use. |
| Molecular Formula |
C37H27N3NA2O9S3
|
|---|---|
| Molecular Weight |
799.80
|
| Exact Mass |
799.07
|
| CAS # |
28983-56-4
|
| PubChem CID |
14513727
|
| Appearance |
Light brown to brown solid powder
|
| LogP |
10.317
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
12
|
| Rotatable Bond Count |
8
|
| Heavy Atom Count |
54
|
| Complexity |
1620
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC(=[NH+]C2=CC=C(C=C2)S(=O)(=O)[O-])C=CC1=C(C3=CC=C(C=C3)NC4=CC=C(C=C4)S(=O)(=O)O)C5=CC=C(C=C5)NC6=CC=C(C=C6)S(=O)(=O)[O-].[Na+].[Na+]
|
| InChi Key |
MCPLVIGCWWTHFH-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2
|
| Chemical Name |
disodium;4-[4-[[4-(4-sulfoanilino)phenyl]-[4-(4-sulfonatophenyl)iminocyclohexa-2,5-dien-1-ylidene]methyl]anilino]benzenesulfonate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 20 mg/mL (25.01 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 16.67 mg/mL (20.84 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2503 mL | 6.2516 mL | 12.5031 mL | |
| 5 mM | 0.2501 mL | 1.2503 mL | 2.5006 mL | |
| 10 mM | 0.1250 mL | 0.6252 mL | 1.2503 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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