| Size | Price | |
|---|---|---|
| Other Sizes |
| ADME/Pharmacokinetics |
Absorption, Distribution and Excretion
...CATIONIC DYES PENETRATE INTO CELLS & STAIN CELL NUCLEI MORE READILY THAN DO ANIONIC DYES. |
|---|---|
| References | |
| Additional Infomation |
Safranin O is an organic chloride salt having 3,7-diamino-2,8-dimethyl-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria. It has a role as a fluorochrome and a histological dye. It contains a 3,7-diamino-2,8-dimethyl-5-phenylphenazin-5-ium.
See also: Phenosafranine (annotation moved to). |
| Molecular Formula |
C20H19CLN4
|
|---|---|
| Molecular Weight |
350.84
|
| Exact Mass |
350.129
|
| CAS # |
477-73-6
|
| Related CAS # |
69882-16-2
|
| PubChem CID |
2723800
|
| Appearance |
Purple to black solid powder
|
| Density |
1.00 g/mL at 20 °C
|
| Flash Point |
46 °C
|
| Index of Refraction |
n20/D 1.339
|
| LogP |
1.612
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
25
|
| Complexity |
405
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
OARRHUQTFTUEOS-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
|
| Chemical Name |
3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 25 mg/mL (71.26 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (7.13 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8503 mL | 14.2515 mL | 28.5030 mL | |
| 5 mM | 0.5701 mL | 2.8503 mL | 5.7006 mL | |
| 10 mM | 0.2850 mL | 1.4252 mL | 2.8503 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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